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Amazon Shop for: ½ Pint Jars, Aquarium Heater, Oyster Shell, Vermiculite, Wild Bird Seed

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InvisibleFungusMan
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OVER INOCULATION, Lets see if it works
    #4543390 - 08/16/05 08:19 PM (11 years, 3 months ago)

If you remember, I made a post 3 days ago called "over inoculation?".
In that post, I told how I used 5cc's in a half pint jar, for a total of 6 jars and 3 syringes of 3 diff strains.Golden Teacher, Treasure Coast, And Cub b+.

Here is the update. The Golden Teacher jars are about 3% colonized, the Treasure Coast is about 1% colonized, and no signs so far with the Cub b+'s. The jars have so far been incubated at room temperature (about 76~F).

I am using a heating pad to bring the temps to about 85~F. They have breathable filters, and I think the substrate is a mix of birdseed, millet, vermiculite, and oyster shell. I will update daily. Pics are soon to come hopefully. For now, Ill have to use my crappy webcam.The first 2 pics are of the Golden Teachers.



bottom of jar



same



Edited by FungusMan (08/17/05 08:55 PM)


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InvisibleFungusMan
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Re: UPDATE ON OVER INOCULATION [Re: FungusMan]
    #4543645 - 08/16/05 09:33 PM (11 years, 3 months ago)

I see alotta peeps looking at this. Let me know if I should keep updating.This is my first attempt with cakes btw.


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Re: UPDATE ON OVER INOCULATION [Re: FungusMan]
    #4543658 - 08/16/05 09:36 PM (11 years, 3 months ago)

Oh.BTW, the heating pad im using has a 2HR auto shut-off. SUX ASS!!!!!
I have to go push the button every 2 hours lol. But, it's keeping a stable 85`F.


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OfflineTweexican
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Re: UPDATE ON OVER INOCULATION [Re: FungusMan]
    #4543667 - 08/16/05 09:39 PM (11 years, 3 months ago)

what were the red circles for?


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OfflineBasidiomycete
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Re: UPDATE ON OVER INOCULATION [Re: FungusMan]
    #4543668 - 08/16/05 09:39 PM (11 years, 3 months ago)

if you keep updating, then theres just one more grow log for a newbie to learn from. So id say yes. Also, have you tried to figure out a way to bypass the 2hr auto shut-off?


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Re: UPDATE ON OVER INOCULATION [Re: Basidiomycete]
    #4543676 - 08/16/05 09:42 PM (11 years, 3 months ago)

Nope. I was just thinking about that though. BUT, it does keep me from burning my house down with it :smile:


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OfflineHoss
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Re: UPDATE ON OVER INOCULATION [Re: FungusMan]
    #4543689 - 08/16/05 09:46 PM (11 years, 3 months ago)

Quote:

FungusMan said:
I see alotta peeps looking at this. Let me know if I should keep updating.This is my first attempt with cakes btw.




We're just looking at your avatar, bro.  :thumbup: :wink:


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Re: UPDATE ON OVER INOCULATION [Re: Hoss]
    #4543694 - 08/16/05 09:48 PM (11 years, 3 months ago)

LOL. Thats a bad pic tho. Ill try to get a better one. lol maybe I should start a poll. BUSH Or BEWBEEZ    :smile:


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OfflineHoss
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Re: UPDATE ON OVER INOCULATION [Re: FungusMan]
    #4543704 - 08/16/05 09:50 PM (11 years, 3 months ago)

PM me with all your media... I'll be the judge.


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Re: UPDATE ON OVER INOCULATION [Re: Hoss]
    #4543715 - 08/16/05 09:53 PM (11 years, 3 months ago)

Lol. unfortunately, this ones all I have at the moment. That will change tho. :smile:


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Re: UPDATE ON OVER INOCULATION [Re: Hoss]
    #4543716 - 08/16/05 09:53 PM (11 years, 3 months ago)

shaved, or unshaved...bush that is


ok, so not to get off topic you said
>I think the substrate is a mix of birdseed, millet, vermiculite, and oyster shell.

so, you aren't really sure the substrate? where did you come up with that forumula?


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Re: UPDATE ON OVER INOCULATION [Re: CLUTCH]
    #4543722 - 08/16/05 09:56 PM (11 years, 3 months ago)

I bought the jars already full of substrate from mycomonsters.


Oh, and SHAVED of coarse!!!!!! :rockon:


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Offlinefrostynostrils
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Re: UPDATE ON OVER INOCULATION [Re: FungusMan]
    #4543838 - 08/16/05 10:23 PM (11 years, 3 months ago)

Bush or boobies? I vote for both.
I got an aquarium heater off of eBay for very cheap, which would let you set up the two-tank incubator.
http://www.shroomery.org/index/par/7961
Then you can just set it and forget it.


--------------------
"Smear the paint till the colors run,
It's nearly time, I'm nearly done
Flaming, orange, open sky!"
- Cherry Poppin' Daddies


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InvisibleDragonaut
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Re: UPDATE ON OVER INOCULATION [Re: FungusMan]
    #4544122 - 08/16/05 11:32 PM (11 years, 3 months ago)

I'm glad to see I didn't discourage you. People actually thought I was being mean to you in the other thread. That wasn't my intent.

It's not a bad idea to leave things be and see how they work out. Hopefully you will NOT get contams. But you never know, with the amounts of water you injected in your jars. How wet did the jars look to begin with? Because if they were a little on the drier side, they will have a better chance of pulling through. You can always dunk after they colonize if they are too dry.

Good luck!


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Edited by WI_Shroomer_Skater (08/16/05 11:38 PM)


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Re: UPDATE ON OVER INOCULATION [Re: Dragonaut]
    #4544529 - 08/17/05 01:04 AM (11 years, 3 months ago)

I would continue updating the pics.  Just don't overdo it and post pics every 2 hours. :smile: Personally from those pics, I really can't see much growth but I think 3 more days worth and it will start showing up in the pics as well :smile:

The only thing that can suck with over-inoculation is that you're adding a LOT of moisture into the jar (and such a small jar) with 5cc's of water.  Just warning that it may begin colonizing but bacteria may overgrow and pause the growing.  I hate being halfway through colonization and then it stops.  I give it a few weeks, open the jar and BAM...the sour shitty smell hits me in the face.  Hurts every time :smile:


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Re: UPDATE ON OVER INOCULATION [Re: Sillypcybin]
    #4547432 - 08/17/05 06:29 PM (11 years, 3 months ago)

Ok. Results of day #4. Golden Teacher jars are showing nice growth.Seeing nice strandy "ball" myc in Treasure Coast about the size of a pencil lead. No noticeable difference in the Cub B+ jars.

Golden Teacher (day #4)




growth on Treasure Coast


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Re: UPDATE ON OVER INOCULATION [Re: FungusMan]
    #4548741 - 08/17/05 10:39 PM (11 years, 3 months ago)

Like I said before tho, after I found out I fucked up, I realised that this would be a good learning experience for me and maybe even some others.


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Re: UPDATE ON OVER INOCULATION [Re: FungusMan]
    #4552498 - 08/18/05 06:55 PM (11 years, 3 months ago)

Day 5 after inoculation. The pic of GT is bottom of jar.As of now, it is the most colonised. The Cub+ surprised me today. Starting to see evidence of growth on them finally.

Golden Teacher(bottom of jar)


Treasure Coast


Psilocybe Cubensis B+


Will update tommorrow.


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Re: UPDATE ON OVER INOCULATION [Re: FungusMan]
    #4552581 - 08/18/05 07:23 PM (11 years, 3 months ago)

Bump to the nuggett!


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Re: OVER INOCULATION, Lets see if it works [Re: FungusMan]
    #4552729 - 08/18/05 08:13 PM (11 years, 3 months ago)

bump


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