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Offlinegreatlakes
Stranger
Registered: 07/25/05
Posts: 277
Last seen: 17 years, 8 months
Myc to LC?
    #4530756 - 08/13/05 04:47 PM (18 years, 8 months ago)

Can you take myc and make a LC from it,If i was to take a little myc from a jar(WBS) and add it to a LC would that work or is there a link i can learn more about doing that?

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peon

Registered: 04/02/05
Posts: 3,022
Loc: somewhere else
Re: Myc to LC? [Re: greatlakes]
    #4530788 - 08/13/05 05:03 PM (18 years, 8 months ago)

yes you could do that, you need to be very sterile about it though. a piece of colonized myc or a piece of tissue from a shroom will work in an lc.
you could take the piece of myc and dip it into some h2o2 and then put it in the lc just to give you some extra protection while transferring.
best done in a glove box or in front of a flow hood.
good luck
peace

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Offlinegreatlakes
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Registered: 07/25/05
Posts: 277
Last seen: 17 years, 8 months
Re: Myc to LC? [Re: backupwards]
    #4530819 - 08/13/05 05:10 PM (18 years, 8 months ago)

thanks man

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peon

Registered: 04/02/05
Posts: 3,022
Loc: somewhere else
Re: Myc to LC? [Re: greatlakes]
    #4530824 - 08/13/05 05:13 PM (18 years, 8 months ago)

no prob, just remember to be very sterile so you don't end up with a jar of contam'd lc.
peace

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Offlinegreatlakes
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Registered: 07/25/05
Posts: 277
Last seen: 17 years, 8 months
Re: Myc to LC? [Re: backupwards]
    #4530872 - 08/13/05 05:31 PM (18 years, 8 months ago)

ok,i'v had no problems with my first 12 jars they all turned out contam free but i tried a G2G with 9 jars and 3 of them got contams but the other ones are looking real good no signs of contams anywhere
Does anyone happen to have a link to share with me for more info on Myc to Lc

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peon

Registered: 04/02/05
Posts: 3,022
Loc: somewhere else
Re: Myc to LC? [Re: greatlakes]
    #4530907 - 08/13/05 05:48 PM (18 years, 8 months ago)

apply this tek to the lc, it is the only one i have found thus far.

http://www.shroomery.org/index/par/8002

peace

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OfflinekronnyQ
SuperstudExtraordinaire
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Registered: 07/22/04
Posts: 2,488
Loc: Anytown USA
Last seen: 9 days, 3 hours
Re: Myc to LC? [Re: backupwards]
    #4530986 - 08/13/05 06:38 PM (18 years, 8 months ago)

Opening jars is quite risky without a glovebox/ flowhood, I don't see why you just don't inject spores into your LC it's 20 times safer.

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Offlinegreatlakes
Stranger
Registered: 07/25/05
Posts: 277
Last seen: 17 years, 8 months
Re: Myc to LC? [Re: kronnyQ]
    #4531008 - 08/13/05 06:52 PM (18 years, 8 months ago)

ya you might be right i am just getting all the info i can on things thats all thanks for the tips guys I think I will wait for my spores and not take a chance

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