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Invisibletony8404
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What method works best for inoculation
    #4525954 - 08/12/05 11:44 AM (12 years, 1 month ago)

Okay this past time i inoculated three jars using the polyfil method.. i took the lid and put a 1/4 hold in middle and stuffed it with poly. and i was gonna inoculate through 4 small holes at edge of lid. but then thought it was easier to inject through the polyfil.. so i took some electric tape over the four small holes.. but since i did that 2 jars contamed and only one made it. so im wondering since i handled the polyfil with out gloves on could this have been the reason two jars got contamed? now im thinking should i use the four hole method this time with electric tape meaning the holes have the tape on them and just put needle through the tape or peel tape off and then replace with electric tape? and should i use some other tape besides electric?


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OfflineInsight
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Re: What method works best for inoculation [Re: tony8404]
    #4526339 - 08/12/05 02:44 PM (12 years, 1 month ago)

What about using silicone instead of tape?


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Invisiblesrgtm1a
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Re: What method works best for inoculation [Re: tony8404]
    #4526426 - 08/12/05 03:06 PM (12 years, 1 month ago)

there is no need for all of that IMO. I basically use 3m tape, tape over the holes, PC, peel the tape off, wipe the lid with rubbing alcohol, prep the needle and inject. I never used tyvek or polyfil or any of that other suff....never got contams.

btw. electrical tape will not stand up to pcing...it won't hold onto that stuff once moisture hits it.

It's just growing mushrooms....keep it simple.


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Invisibletony8404
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Re: What method works best for inoculation [Re: srgtm1a]
    #4526456 - 08/12/05 03:18 PM (12 years, 1 month ago)

when u say 3m tape do you mean masking tape or can i use the clear tape as well>?


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Invisiblesrgtm1a
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Re: What method works best for inoculation [Re: tony8404]
    #4526609 - 08/12/05 03:54 PM (12 years, 1 month ago)

you should be able to use masking tape. I use a 3m tape that is textured...couldn't tell you the type...it basically has a "criss cross" design in the tape that helps it stick better, but yeah, clear tape is fine. like that packing tape.


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Invisibletony8404
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Re: What method works best for inoculation [Re: srgtm1a]
    #4526767 - 08/12/05 04:33 PM (12 years, 1 month ago)

i know what ya talkin there... i just dont have masking tape .. i have 3m clear packing tape


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Invisiblesrgtm1a
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Re: What method works best for inoculation [Re: tony8404]
    #4527468 - 08/12/05 07:44 PM (12 years, 1 month ago)

that should work fine.


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