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OfflineAiko Aiko
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Agar&LC
    #4521276 - 08/11/05 07:31 AM (11 years, 4 months ago)

Is it feasable to turn agar into an LC. And what would be the plate:liquid ratio. Thanx. :grin:


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Man says, "God, show me and I will believe." God says, "Believe and I will show you."


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Invisibleagar
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Re: Agar&LC [Re: Aiko Aiko]
    #4521325 - 08/11/05 09:05 AM (11 years, 4 months ago)

Sure you can. It just turns it into a slurry.
One plate to a pint of sterilized water is a decent ratio.


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OfflineAiko Aiko
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Re: Agar&LC [Re: agar]
    #4521333 - 08/11/05 09:14 AM (11 years, 4 months ago)

Can I use that to knock up a substrate, or is it better suited to make more LC's?


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Man says, "God, show me and I will believe." God says, "Believe and I will show you."


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OfflineHotnuts
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Re: Agar&LC [Re: Aiko Aiko]
    #4521355 - 08/11/05 09:41 AM (11 years, 4 months ago)

Sure. You can use it for any of the above.


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OfflineAiko Aiko
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Re: Agar&LC [Re: Hotnuts]
    #4521373 - 08/11/05 10:00 AM (11 years, 4 months ago)

Thanx for the help fellas. Peace!


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OfflineInsight
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Re: Agar&LC [Re: Aiko Aiko]
    #4533432 - 08/14/05 12:17 PM (11 years, 3 months ago)

I did mine by 1 plate to 1/2 pint water, ran it through the blender for a couple seconds and now its sits rebuilding.


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Invisiblemycofile
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Re: Agar&LC [Re: Insight]
    #4533984 - 08/14/05 03:42 PM (11 years, 3 months ago)

Just want to add that I would prefer using a LM rather than pure water to blend the agar in, then let it grow for 2-4 days before shooting. This is the method discussed in GG&MM, and stamets reports improved performance over just blending the agar and shooting it.

Not that just blending agar doesn't work though....


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Invisibleagar
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Re: Agar&LC [Re: mycofile]
    #4535183 - 08/14/05 09:43 PM (11 years, 3 months ago)

I agree with that. As, the more myc, the merrier with LC's.


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Invisiblemycofile
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Re: Agar&LC [Re: agar]
    #4538761 - 08/15/05 06:15 PM (11 years, 3 months ago)

Not just more, but also actively growing in three dimensions rather than just two...


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"From a certain point of view"
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PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.


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OfflineAiko Aiko
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Re: Agar&LC [Re: mycofile]
    #4541386 - 08/16/05 07:29 AM (11 years, 3 months ago)

I'm sorry im not sure I know what LM is. Do you mean add the plate to a nutrient solution (i.e. karo) as opposed to plain water?


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Invisiblemycofile
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Re: Agar&LC [Re: Aiko Aiko]
    #4542691 - 08/16/05 04:14 PM (11 years, 3 months ago)

Yeah, LM is Liquid Media. Honey, Karo, dextrose, Malt extract, peptone, nutritional yeast etc are all nutrients used in LM recipes.


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"From a certain point of view"
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PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.


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OfflineAiko Aiko
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Re: Agar&LC [Re: mycofile]
    #4543709 - 08/16/05 09:51 PM (11 years, 3 months ago)

Would you consider any of these better than the others for any specific reason?


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Offlineblackout
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Re: Agar&LC [Re: Aiko Aiko]
    #4545426 - 08/17/05 04:15 AM (11 years, 3 months ago)

Well honey is very easy to find, you can get clear honey which will have very little sediment when sterilized. Honey is also has natural anti-bacterial properties.

Karo is nice and clear, easily got in the US. The clearness allows you to spot contams easier.


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OfflineAiko Aiko
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Re: Agar&LC [Re: blackout]
    #4545780 - 08/17/05 08:09 AM (11 years, 3 months ago)

I just knocked up a couple karo solutions w/ Shiitake and Oyster. I'll let you guys know how it turns out. Thanx for all the suggestions.  :cool: :mushroomgrow:


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Invisiblemycofile
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Re: Agar&LC [Re: Aiko Aiko]
    #4546791 - 08/17/05 03:19 PM (11 years, 3 months ago)

Well cool, but just for the record, I like dextrose (clear as a bell and has none of the unneeded stuff in karo), if you are going to shoot it just as soon as it's colonized. If clarity doesn't matter, I like dextrose with a little nutritional yeast. I've also seen great results mixing light malt extract with dextrose 50/50.

Whatever you use you usually want your sugar concentration around 4%.

Good luck.


--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.


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OfflineBlue Helix
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Re: Agar&LC [Re: mycofile]
    #4645712 - 09/11/05 01:24 PM (11 years, 3 months ago)

I've been using dextrose/light malt extract with great results. I just brew 10g dextrose and 10g light malt extract in 500 to 700 mls of RO water by putting the dry components in a coffee filter and letting the coffee machine make the 500ml brew. Then I pour the brew in a quart jar (adding the stir magnet covered with a small section of cushion rubber tubing) and cover with a metal lid and filter patch and metal band over it all. The metal lid has an offset large hole covered with medical cloth tape (the stuff you wrap ankles with and stuff). I pressure cook the whole thing for 20 minutes, then inject a couple or few CCs of spores or liquid culture into the jar through the cloth tape, quickly replacing the filter lid immediately after I pull out the needle (this is done in my kitchen which is not clean). I constant stir with a stirrer at about 200-500 RPM for a week. The resulting culture is very thick and develops fast. In one week it was far thicker--maybe three times as dense--than the liquid culture I got from spore works for my Shiitake, for example. I've done Shiitake, cubes, and Stipticus Panellus (glowing wood lover) this way and all grew out very dense and fast. Of course cubes grow the very fastest (like 4 to 5 days to a very dense culture if temp is controlled around 85F). If I added a pinch of hardwood sawdust to my wood lover LCs, they'd probably do just as well as the cubes but I like the clarity.


When I say fast here is what I mean: below is a picture of a cube LC after about 3.5 days of constant stir started with a single milliliter (1cc) of liquid culture (this is about half as dense as I would rather use it at 5 days):



Edited by Blue Helix (09/11/05 01:24 PM)


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OfflineAiko Aiko
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Re: Agar&LC [Re: Blue Helix]
    #4658531 - 09/14/05 07:37 AM (11 years, 2 months ago)

WoW, pretty impressive. BTW my LC's turned out OK. Took about three weeks and there nowhere as thick as that. Oh well, definet room for improvement. A stir plate and dextrose just moved a little further up my prority list.


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Man says, "God, show me and I will believe." God says, "Believe and I will show you."


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InvisibleTCatz
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Re: Agar&LC [Re: Aiko Aiko]
    #4677804 - 09/18/05 04:18 PM (11 years, 2 months ago)

what size stir bar is recommended for use in mason jars?
I'm looking at sizes available from 1/2' to 2"


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Edited by TCatz (09/18/05 04:35 PM)


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InvisibleTCatz
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Re: Agar&LC [Re: TCatz]
    #4752123 - 10/04/05 12:52 AM (11 years, 2 months ago)

when shooting for 4% mixure of sugar to water for LC
do you mean 4% by volume? weight?


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Invisiblemycofile
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Re: Agar&LC [Re: TCatz]
    #4754685 - 10/04/05 04:15 PM (11 years, 2 months ago)

weight.


--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.


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Amazon Shop for: ½ Pint Jars, Agar, Malt Extract, Paul Stamets

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

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