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Offlinedroptopsunfire03
Wandering Deity

Registered: 08/04/05
Posts: 174
Last seen: 8 years, 11 months
Sterilizing/reusing syringes? can it be done?
    #4518816 - 08/10/05 06:01 PM (11 years, 3 months ago)

Hey guys i have successfully grown for the first time a nice set of colonized jars on BRF, with a 66% contam rate( i think i got the first set of 4 too hot) I now have them outdoors in a compost/manure mixture, and they have been out there only 2 days. I saved one healthy jar, and am looking to make some LC for growing indorr when winter hits. I had read a tek on using a syringe to inject some LC into a healthy colonized jar shaking it and sucking back out water/LC miz with living mycelium in it, and using that to inoculate the LC rather than a new syringe. Now what i need to know is, i have the one original syringe i got my spores in, can i sterilize this and use it for the above stated purpose or do i have to buy new?? If i need new where can i get the without attracting too much attention? Thanks guys.

P.S. Expect Pics soon;)


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Offlinescatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
Loc: Flag
Last seen: 3 years, 10 months
Trusted Cultivator
Re: Sterilizing/reusing syringes? can it be done? [Re: droptopsunfire03]
    #4518869 - 08/10/05 06:19 PM (11 years, 3 months ago)

Yes you can, PC the syringe for 15 minutes, or boil for 15-30 minutes.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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InvisibleRoadkill
Retired Shroomery Mod
Male User Gallery

Registered: 12/11/01
Posts: 22,598
Loc: Snoqualmie, Wa.
Trusted Cultivator
Re: Sterilizing/reusing syringes? can it be done? [Re: droptopsunfire03]
    #4518876 - 08/10/05 06:20 PM (11 years, 3 months ago)

yes it can be re-used.

you boil some water...

suck in some boiled water into the syringe...

shoot the water out...into the sink.

repeat the above...three times.

The last time leave the water in the syringe.

Then wrap the syringe up in alum foil and pressure cook it in your pc.

wa la...sterile syringe with sterile water inside the syringe.

then follow a syringe making tek.

tc


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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Offlinedroptopsunfire03
Wandering Deity

Registered: 08/04/05
Posts: 174
Last seen: 8 years, 11 months
Re: Sterilizing/reusing syringes? can it be done? [Re: Roadkill]
    #4518885 - 08/10/05 06:22 PM (11 years, 3 months ago)

Sweet, thanks guys, i had thought the PC would melt the plastic so was afraid to try it.


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Offlineixitwistedixi
The TwIsTeD One

Registered: 07/22/05
Posts: 967
Last seen: 3 years, 4 months
Re: Sterilizing/reusing syringes? can it be done? [Re: droptopsunfire03]
    #4518894 - 08/10/05 06:25 PM (11 years, 3 months ago)

Thx for clarifying that roadkill i was like "how do you boil plastic w/out it melting?" - maby that can be done as well but i wasnt sure.


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Offlinescatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
Loc: Flag
Last seen: 3 years, 10 months
Trusted Cultivator
Re: Sterilizing/reusing syringes? can it be done? [Re: ixitwistedixi]
    #4518926 - 08/10/05 06:34 PM (11 years, 3 months ago)

Of course you can boil or PC it without melting...there are many very strong plastics that will withstand over 250 degrees. I do believe some syringes will deform, all I've tried so far have worked though (like three or four brands)


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinedroptopsunfire03
Wandering Deity

Registered: 08/04/05
Posts: 174
Last seen: 8 years, 11 months
Re: Sterilizing/reusing syringes? can it be done? [Re: scatmanrav]
    #4518934 - 08/10/05 06:36 PM (11 years, 3 months ago)

What about buying new?? What is a good source?? I want to have a bunch ready in the fridge b4 winter


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Offlinescatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
Loc: Flag
Last seen: 3 years, 10 months
Trusted Cultivator
Re: Sterilizing/reusing syringes? can it be done? [Re: droptopsunfire03]
    #4518944 - 08/10/05 06:38 PM (11 years, 3 months ago)



--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


Post Extras: Print Post  Remind Me! Notify Moderator
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Amazon Shop for: ½ Pint Jars, Brown Rice Flour

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