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Amazon Shop: ½ Pint Jars, Brown Rice Flour, Vermiculite

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OfflineImpulse101
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no pc
    #4510230 - 08/08/05 01:32 PM (11 years, 11 months ago)

Yes i know this question has been asked 1000 times but i searched shroomery, google, and yahoo and no answers...

can you use and grain substrate without a pc... dont have one at the moment...


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"Take me koalas back jack... take my koalas back, he lives somwhere out on the track mack... so take me koalas back"

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Offlinelowdominion
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Re: no pc [Re: Impulse101]
    #4510247 - 08/08/05 01:38 PM (11 years, 11 months ago)

without a pc grains are pretty much a contam breedng ground


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InvisibleFooManM
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Re: no pc [Re: Impulse101]
    #4510286 - 08/08/05 01:51 PM (11 years, 11 months ago)

Look up fractional sterilization. This involves heating the grains several times to get endospores to germinate, then killing them in seperate steps.


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Offlinescatmanrav
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Re: no pc [Re: lowdominion]
    #4510290 - 08/08/05 01:52 PM (11 years, 11 months ago)

Of course you can, brown rice flour and verm, its caled the PF Tek and its listed all over.

You cant use WHOLE grains without a PC though. If you dont want to get one, I suggest the PF Tek, spawned to horse poo...cant beat horse poo as a substrate.


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"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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InvisibleFooManM
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Re: no pc [Re: Impulse101]
    #4510302 - 08/08/05 01:56 PM (11 years, 11 months ago)

You can read up on whole grain fractional sterilization here.


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Offlinescatmanrav
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Re: no pc [Re: FooMan]
    #4510345 - 08/08/05 02:17 PM (11 years, 11 months ago)

Yeah, that might work, personally never tried it, but it seems like a pain in the ass..you probably could do it that way though.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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InvisibleFooManM
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Re: no pc [Re: scatmanrav]
    #4510446 - 08/08/05 02:42 PM (11 years, 11 months ago)

Quote:

scatmanrav said:
Yeah, that might work, personally never tried it, but it seems like a pain in the ass..you probably could do it that way though.




Oh, I'm not saying that I would want to do it myself, but if you really want to use whole grains and you don't have a pc, I guess this is your only alternative. I agree with you Scat. It would be a ROYAL pain in the ass, but considering that this hobby already has me playing with horse shit, I can't say that anything is above trying :smirk:


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OfflinePinx
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Re: no pc [Re: FooMan]
    #4517996 - 08/10/05 01:47 PM (11 years, 11 months ago)

Whole grains without PC shouldn't be a prob, just use peroxide solution instead of water when preparing the glasses. When the peroxide had enough time to kill everything then boil the glasses. Since peroxide isn't very stable above room temperature it should break up and leave nothing, but a sterile glass with grain and water.

btw one could add some iron powder bc iron works as a catalyst for the breakup of H2O2


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Invisibleagar
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Re: no pc [Re: Pinx]
    #4518013 - 08/10/05 01:51 PM (11 years, 11 months ago)

Quote:

Pinx said:
Whole grains without PC shouldn't be a prob, just use peroxide solution instead of water when preparing the glasses. When the peroxide had enough time to kill everything then boil the glasses. Since peroxide isn't very stable above room temperature it should break up and leave nothing, but a sterile glass with grain and water.

btw one could add some iron powder bc iron works as a catalyst for the breakup of H2O2




Frac sterilization works about 25% of the time. Meaning, out of 10 jars, you may get 3 without contamination.


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OfflinePinx
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Re: no pc [Re: agar]
    #4518061 - 08/10/05 02:07 PM (11 years, 11 months ago)

excuse me but whats the point with the quote?

the methode i suggested uses the fact that H2O2 can be used to kill microorganisms, and after that it can be cooked, so that there is nothing left but water H2O

btw about your sig:

Dont let your fear win over reason, cmon you know that one cant fight terror with an army. Ever heard that fighting for peace is like fucking for virginity.


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Offlinescatmanrav
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Re: no pc [Re: Pinx]
    #4518780 - 08/10/05 05:50 PM (11 years, 11 months ago)

>the methode i suggested uses the fact that H2O2 can be used to kill microorganisms

Except thats not a fact, its incorrect. Peroxide will not sterilize grains, what will happen is peroxidase will be produced and break down the hydrogen peroxide into nothing.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


Post Extras: Print Post  Remind Me! Notify Moderator
OfflinePinx
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Registered: 06/20/05
Posts: 56
Last seen: 10 years, 1 month
Re: no pc [Re: scatmanrav]
    #4520959 - 08/11/05 03:28 AM (11 years, 11 months ago)

You can kill MOs with peroxide i agree that maybe not all organisms die and peroxidase may be produced, but i dont think that this will happen with concentrations like 30% peroxide.
If I am wrong I am sorry for giving bad advice.


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InvisiblePrisoner#1
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Re: no pc [Re: Pinx]
    #4521294 - 08/11/05 07:58 AM (11 years, 11 months ago)

it still wont sterilize grain suitably


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Amazon Shop: ½ Pint Jars, Brown Rice Flour, Vermiculite

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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