Home | Community | Message Board


Azarius
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: ½ Pint Jars, Agar, HEPA Filter, Laminar Flow Hood, Petri Dish, Pressure Cooker, Wild Bird Seed

Jump to first unread post. Pages: 1
Offlinetokey666
Lamb of God
 User Gallery

Registered: 08/05/05
Posts: 630
Loc: Your Mom
Last seen: 9 years, 10 months
construction/sterilzation of glove box..... (suggestions?)
    #4507565 - 08/07/05 06:17 PM (11 years, 4 months ago)

Hey everybody. I wanted to get some different opinions about the contruction of a glove box and how to sterilize it.

I found a clear container at wally world with a clear lid as well. It a little too big, but better be too big than too small. Its heavy plastic and would work great.

My question is though, do I really need to filter the thing by placing a fan, HEPA filter and other random electical contraptions in/on there? (I would like portability.)

It would only be used for syringe creation from prints and inoculation. Besides that, don't you have to open the lid, and whatnot anyway and let bacteria in when you are putting the items in there to work with?

Also, how should I sterilize it? I can't fit this beast in a pressure cooker. lol. Should I just rub the inside down with alcohol and/or spray it with lysol, then shut the lid asap? Or is there another/better method?

OR, is this just overkill and should I just not worry about it. I do have to say though, I live in an old farm house (not hillybilly) but old, so there are always dust particles floating around. And I'm sure bacteria.

Thanks for all/any help!


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineHippieChick
Chicks can do it too!
Female User Gallery

Registered: 02/20/05
Posts: 5,958
Loc: Midwest
Last seen: 3 days, 10 hours
Trusted Cultivator
Re: construction/sterilzation of glove box..... (suggestions?) [Re: tokey666]
    #4507614 - 08/07/05 06:28 PM (11 years, 4 months ago)

No you don't have to use fans and HEPA's and the such. I did but I love to build things,lol.



Alcohol and or Lysol will work just fine. Just give it a little time , about 10 minutes or so after spraying for it to settle.

I did a positive flow box so I can load it and not have to keep sterilizing it. That can be a pain when you are working a 100 jars or so. I load and unload through a hole in the side,



I actually do all my inoculations outside the box now. I've done 75 quarts WBS outside the box and haven't lost one yet :grin: But I do my jars different then anyone else I've seen.

Good Luck

Peace,Love,Happiness and Harmony.
:heart: Hippie Chick  :mushroom2:


--------------------
Peace,Love and Happiness
:heart: HC :mushroom2:

Freedoms just another word for nothing left to lose..............

I LUV My Greenhouse
http://www.shroomery.org/forums/showflat.php/Number/5545848#5545848

My First Pans
http://www.shroomery.org/forums/showflat.php/Number/6212058#6212058


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineRogerRabbitM
Bans for Pleasure
 User Gallery

Registered: 03/26/03
Posts: 41,968
Loc: USA Mountain Northwest
Last seen: 2 months, 15 days
Trusted Cultivator
Re: construction/sterilzation of glove box..... (suggestions?) [Re: HippieChick]
    #4507700 - 08/07/05 06:54 PM (11 years, 4 months ago)

Still air is what you want with a glovebox. Sterility is not important either as you don't dump your spawn out on the floor of the glovebox. Just cut a couple of arm holes in your container, and either glue gloves or tyvek sleeves in place. Soap and water is sufficient to clean a glovebox. Don't use alcohol or lysol if you plan to use an alcohol flame to sterilize your needles/scalpel. Just before making transfers, it helps to spray a few squirts with a fine mister(water only) as the mist will settle any contaminants that may be airborne inside the box.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinetokey666
Lamb of God
 User Gallery

Registered: 08/05/05
Posts: 630
Loc: Your Mom
Last seen: 9 years, 10 months
Re: construction/sterilzation of glove box..... (suggestions?) [Re: HippieChick]
    #4507716 - 08/07/05 07:03 PM (11 years, 4 months ago)

SWEET! You have a shower inside your chamber! :smile:) I love it! I'm definately going that route (suction cups and such.)

And EXCELLENT thinking using the pvc pipes and hose clamps. I was thinking about using silicone on the outside of the box and press the gloves to them (rubber side to plastic) so they could stick. But after stretching and bending, it may come loose.

Question though. Positive flow? Whats that mean? The fan is sucking air out and the intake to the box is the hole where you pass stuff?  Like a passive intake system?  And wouldn't that just suck all the bacteria in?  lol.

Thanks Hippie Chick! Great thinkin!

Addition: Sounds like a good plan RogerRabbit. Makes sense.  Thanks!  Seems like an easier method.


Edited by tokey666 (08/07/05 07:09 PM)


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineHippieChick
Chicks can do it too!
Female User Gallery

Registered: 02/20/05
Posts: 5,958
Loc: Midwest
Last seen: 3 days, 10 hours
Trusted Cultivator
Re: construction/sterilzation of glove box..... (suggestions?) [Re: tokey666]
    #4507937 - 08/07/05 08:27 PM (11 years, 4 months ago)

Thanks.

The fan pulls air from the outside through a HEPA filter. Hooked it up to a nine volt. Guess its portable,lol.



All my holes are sealed with silicon. The top fits tight and I strap it down with 4 pieces of duct tape. Its pretty air tight. So everything is pushed out the loading hole by sterile air, or realclose to sterile. I've never had a contam with jars or anything done in it. :grin: I use it for G2G and syringe making now.

Peace,Love,Happiness and Harmony.
:heart: Hippie Chick  :mushroom2:


--------------------
Peace,Love and Happiness
:heart: HC :mushroom2:

Freedoms just another word for nothing left to lose..............

I LUV My Greenhouse
http://www.shroomery.org/forums/showflat.php/Number/5545848#5545848

My First Pans
http://www.shroomery.org/forums/showflat.php/Number/6212058#6212058


Post Extras: Print Post  Remind Me! Notify Moderator
InvisiblePrisoner#1M
Even Dumber ThanAdvertized!
 User Gallery

Registered: 01/22/03
Posts: 191,681
Loc: Pvt. Pubfag NutSuck
Trusted Cultivator
Re: construction/sterilzation of glove box..... (suggestions?) [Re: HippieChick]
    #4508188 - 08/07/05 10:04 PM (11 years, 4 months ago)

if it works for you thats good, like Roger I recomend staying away from positive pressure unless you're using a flow hood, you tend to be much more detail oriented than most people so I can see why yours works

btw... thats a 6 vilt lantern battery  :smirk:


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineHippieChick
Chicks can do it too!
Female User Gallery

Registered: 02/20/05
Posts: 5,958
Loc: Midwest
Last seen: 3 days, 10 hours
Trusted Cultivator
Re: construction/sterilzation of glove box..... (suggestions?) [Re: Prisoner#1]
    #4508514 - 08/07/05 11:26 PM (11 years, 4 months ago)

Quote:

Prisoner#1 said:

btw... thats a 6 vilt lantern battery  :smirk:





Thanks Pris. Excuse the typo. It's not a 9 volt or 6 vilt, its a 6 volt,lol.

Peace,Love,Happiness and Harmony.
:heart: Hippie Chick  :mushroom2:

I'm getting ready to start working with agar. I'm gonna leave a culture tube in it, open, for a day with it running to see what happens. Kinda really test it out. :thumbup:


--------------------
Peace,Love and Happiness
:heart: HC :mushroom2:

Freedoms just another word for nothing left to lose..............

I LUV My Greenhouse
http://www.shroomery.org/forums/showflat.php/Number/5545848#5545848

My First Pans
http://www.shroomery.org/forums/showflat.php/Number/6212058#6212058


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibledeanofmean
mycophagous

Registered: 12/07/02
Posts: 2,017
Loc: PNW
Re: construction/sterilzation of glove box..... (suggestions?) [Re: tokey666]
    #4509683 - 08/08/05 07:10 AM (11 years, 4 months ago)

as RR said, 0 air movement is what is wanted. move slowly and deliberately so as not to stir up the air.
especially if you are cleaning specimens on agar. if you use a fan the moment you lift the lid of the petri dish, mold spores will contaminate everything. :frown:
i clean  the glove box, and everything as it goes in w/ bleachy water.
spraying w/ 35% isopropyl provides an extra buffer just in case  anything is accidentally dropped on the floor :smile:


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleRoadkill
Retired Shroomery Mod
Male User Gallery

Registered: 12/11/01
Posts: 22,598
Loc: Snoqualmie, Wa.
Trusted Cultivator
Re: construction/sterilzation of glove box..... (suggestions?) [Re: tokey666]
    #4509725 - 08/08/05 07:53 AM (11 years, 4 months ago)



--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop for: ½ Pint Jars, Agar, HEPA Filter, Laminar Flow Hood, Petri Dish, Pressure Cooker, Wild Bird Seed

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* Glove Box goldtop 540 1 09/18/01 08:58 AM
by Anno
* Simple filtered air glove box construction with Pics
( 1 2 all )
Peterthinks 4,365 22 02/24/08 12:16 AM
by Peterthinks
* My Glove Box Construction – OK? fromthemoon 8,207 10 06/09/11 11:03 AM
by woodylover
* simple glove box sleepingbigguy 899 3 12/15/04 03:06 PM
by mushrea
* Dual purpose Glove Box/Incubation Chamber namgrass 934 0 11/11/05 10:42 AM
by namgrass
* glove box stxlacrosse022 1,877 18 09/19/07 11:02 PM
by stxlacrosse022
* Syringe Sterilization In Glove Box *Newbie* DK1 971 2 01/04/05 12:21 PM
by KaptKid
* The shitest glove box ever. *DELETED* fantastic dan 300 1 10/17/08 01:46 AM
by noobieshroomie

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Magash, Shroomism, george castanza, Prisoner#1, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, EvilMushroom666, cronicr, PussyFart
677 topic views. 20 members, 84 guests and 25 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
SoulSpeciosa Kratom
Please support our sponsors.

Copyright 1997-2016 Mind Media. Some rights reserved.

Generated in 0.073 seconds spending 0.007 seconds on 14 queries.