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Anonymous
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Sterilizing Casing Layer - Need Help ASAP
#4504244 - 08/06/05 03:56 PM (18 years, 7 months ago) |
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Hello everyone..
I'm just about to prepare my first casing, and I was wondering if I could sterilize my casing layer (Coco coir) in a PC without an oven bag. I would add water to it, then just put it in the PC. Will this be okay...will it burn?
Thanks =0)
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johnson657
Stranger
Registered: 08/01/05
Posts: 308
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Re: Sterilizing Casing Layer - Need Help ASAP [Re: ]
#4504446 - 08/06/05 05:29 PM (18 years, 7 months ago) |
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My guess is that it will burn, you could use the microwave instead. Or use a large amount of foil instead of an oven bag.
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LouiseLouise
starstruck
Registered: 11/02/04
Posts: 3,898
Loc: Searching w/my good eye c...
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Re: Sterilizing Casing Layer - Need Help ASAP [Re: ]
#4504481 - 08/06/05 05:43 PM (18 years, 7 months ago) |
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You could put it in jars, leave the top cracked a little. It's not all that important to sterilize the casing layer since it is non-nutritional. I like to mic. it anyway, and I use h2o2 solution to moisten.
GL
-------------------- "That's why you get in close to them, and then take the picture!! Don't be a pussy!" ~CC
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Heinous_Anus
S.D.M.F.
Registered: 08/03/05
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Re: Sterilizing Casing Layer - Need Help ASAP [Re: LouiseLouise]
#4504604 - 08/06/05 06:15 PM (18 years, 7 months ago) |
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If it were in the microwave, how long would you keep it on for?
-------------------- I didn't go to college. I went to Ozzy Osbourne University. - Zakk Wylde
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LouiseLouise
starstruck
Registered: 11/02/04
Posts: 3,898
Loc: Searching w/my good eye c...
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Re: Sterilizing Casing Layer - Need Help ASAP [Re: Heinous_Anus]
#4504620 - 08/06/05 06:21 PM (18 years, 7 months ago) |
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I have a right powerful mic. I put it in for 5 min. then while still in the mic, i place tin foil over the top and transfer it to a place to cool off. That takes a while.
GL
-------------------- "That's why you get in close to them, and then take the picture!! Don't be a pussy!" ~CC
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Heinous_Anus
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Re: Sterilizing Casing Layer - Need Help ASAP [Re: LouiseLouise]
#4504622 - 08/06/05 06:23 PM (18 years, 7 months ago) |
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Alright, thanks for the answer.
I love the quick responses around here.
-------------------- I didn't go to college. I went to Ozzy Osbourne University. - Zakk Wylde
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Nashbar
just strange.... on drugs
Registered: 07/16/05
Posts: 3,536
Loc: strawberry field
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Re: Sterilizing Casing Layer - Need Help ASAP [Re: Heinous_Anus]
#4504638 - 08/06/05 06:33 PM (18 years, 7 months ago) |
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Add a little extra water than normal, it will steam off during heating. 2 times on high for 10 min each for my 30 yr old microwave... stir in between each. Keep it covered, let cool and case.
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srgtm1a
Stranger
Registered: 02/19/05
Posts: 2,625
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Re: Sterilizing Casing Layer - Need Help ASAP [Re: ]
#4504645 - 08/06/05 06:35 PM (18 years, 7 months ago) |
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I never sterilize my coir and don't have contam problems with it.
I have never sterilized a casing layer no matter what it was. I only had a contam once with peat. this is out of a lot of casings.
I wouldn't worry about it if I were you.
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Cryogenicz
what?
Registered: 07/01/04
Posts: 2,421
Loc: Oregon
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Re: Sterilizing Casing Layer - Need Help ASAP [Re: srgtm1a]
#4504769 - 08/06/05 07:25 PM (18 years, 7 months ago) |
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I highly suggest you sterilize any casing or anything that touches your inoculated medium as it could have bacteria, or mold, or whatever crap you dont want in there. I dont understand why people think that since its not nutriative that it is ok to not sterilize it. it can harbor mold or bacteria and cause infections. was your hands anytime you work with your casings, and keep everything that touches them CLEAN. People, its not worth your time to do all of this work and then make simple mistakes like not keeping clean.
-------------------- www.MycoPath.com Mushroom Spawn, Cultures, Fungi Bags, Casings, Master Grain Jars, Bags for In-vitro, Laboratory supplies, and much more! Mushroom Supplies. Fast Turnaround Times. Great Service. orders@mycopath.com enter code shroomery for 10% off product. www.FungiForum.com
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srgtm1a
Stranger
Registered: 02/19/05
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Re: Sterilizing Casing Layer - Need Help ASAP [Re: Cryogenicz]
#4504803 - 08/06/05 07:49 PM (18 years, 7 months ago) |
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"I don't understand why people think that since its not nutriative that it is ok not to sterilize it."
because I never did, and never got contammed, except once, but that was peat(peat sux). I've done quite a few casings as well, no contam problems ever.
Sure, if you want to play it safe, go for it. IMO I really don't think it is necessary, and think it is a waste of time.
and you say "doing all this work" hehehe what work?....this shit's fun.
People get far to paranoid about contams around here. Simple rule, if you work in a clean environment, you have nothing to worry about.
I look at some of these posts at what people do to get rid of possible contams problems and it really blows my mind.
Maybe I have a "Shroom God" looking over my shoulder or something cuz I never do half the stuff i see others doing.
I just PC, and then wipe the surface/instruments down with rubbing alcohol. then flame what needs to be flamed.
No heppa filters, no glove boxes, non of that stuff...and everything for me works out great.
maybe contams just don't like my ora, so they go to other member's homes <-----some hippie shit right there.
Edited by srgtm1a (08/06/05 08:15 PM)
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liveby
Wasted For Time
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Re: Sterilizing Casing Layer - Need Help ASAP [Re: srgtm1a]
#4505164 - 08/06/05 10:04 PM (18 years, 7 months ago) |
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yeah go for a jar , i never had a contam with cococoir untill 3 flush
-------------------- http://www.bruceeisner.com/ -Creating a Sensible Culture
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RogerRabbit
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Re: Sterilizing Casing Layer - Need Help ASAP [Re: liveby]
#4505367 - 08/06/05 11:05 PM (18 years, 7 months ago) |
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A sterilized casing layer will contaminate far sooner than a pasteurized, or even non-treated one. The bacteria in peat is beneficial and helps to protect against molds. The only time you need to be sterile is when preparing your spawn, whether brf or rye or whatever. Once it's fully colonized, you don't want your bulk substrate to be sterile. That's why we only pasteurize straw and casing materials. We want to kill the mold spores, but allow the bacteria to survive. To pasteurize a casing layer, simply bring it to field moisture level, then heat to 140-150F for an hour. Whatever method you use is fine. I discourage microwave use because it will get the material too hot, thus killing the beneficial bacteria. Once they're gone, molds such as trich, aspergillus and penicillium can take hold. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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bandog
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Registered: 01/19/07
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Re: Sterilizing Casing Layer - Need Help ASAP [Re: RogerRabbit]
#8461726 - 05/29/08 09:42 PM (15 years, 9 months ago) |
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But ... if using non-nutritive casing, sterilization is recommended ... right? Since the non-nutritive casing contains neither "good" nor "bad" critters, there is no advantage to pasteurization, and a significant advantage to sterilization.
-------------------- Come in to my parlour, said the spider to the fly ...
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Brainiac
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Re: Sterilizing Casing Layer - Need Help ASAP [Re: bandog]
#8461955 - 05/29/08 10:30 PM (15 years, 9 months ago) |
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Why did you bump a 3 year old thread..
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P.Menace
Contradicting the Status Quo
Registered: 11/17/07
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Re: Sterilizing Casing Layer - Need Help ASAP [Re: Brainiac]
#8462586 - 05/30/08 02:26 AM (15 years, 9 months ago) |
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yeah^ what he said^
I was wondering on the annom post
-------------------- http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Menace roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.
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