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Offlinebent
bent1

Registered: 07/31/03
Posts: 204
Loc: ny
Last seen: 10 years, 3 months
what are all possible reasons for no growth with grain (wbs)?
    #4496380 - 08/04/05 08:49 PM (11 years, 6 months ago)

I had a pint of fully colonized healthy koh sumai super strain, and spawned it to a 5qt spawnbag of wbs that I soaked for 24 hours and pressure cooked for 2.5 hours, before letting cool for a few days and spawning. It's been a week since they've been incubating and no growth. Whenever I use WBS its pretty hit or miss and usually have trouble spawning, what are all possible reasons? I use a lil pressure box type glovebox that works quite well, its just sometimes stuff like dies for no reason.


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OfflineWronguy
Registered: 03/05/05
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Re: what are all possible reasons for no growth with grain (wbs)? [Re: bent]
    #4496497 - 08/04/05 09:18 PM (11 years, 6 months ago)

It might be your incubating temps. Have you tried gently moving the spawn bag around to see if the mycelium is growing in the middle? I've had mycelium do the same thing, but it stuck to the middle because my incubating temps were too low.


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Invisibleagar
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Registered: 11/21/04
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Re: what are all possible reasons for no growth with grain (wbs)? [Re: bent]
    #4496506 - 08/04/05 09:20 PM (11 years, 6 months ago)

There are about 787 reasons. The primary one is usualy whatever you G2G'ed it into - is/was to DRY. Next might be your incubation temps are way off. After that, it might be the ambiant outside rh in whatever you incubate in, is so LOW, the bag is simply drying out - though the filter patch on the bag.


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Offlinebent
bent1

Registered: 07/31/03
Posts: 204
Loc: ny
Last seen: 10 years, 3 months
Re: what are all possible reasons for no growth with grain (wbs)? [Re: agar]
    #4498015 - 08/05/05 02:21 AM (11 years, 6 months ago)

okie dokie, im gonna do it up and get her wet this time! also im gonna measure my temps with a thermometer instead of my hand like some old-school mama.


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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