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PsyWonderCap
Stranger
Registered: 07/19/05
Posts: 73
Last seen: 18 years, 5 months
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I am scared (5 days after injection)
#4489357 - 08/03/05 11:08 AM (18 years, 6 months ago) |
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So today was the 5th day, i waited to see if there were at least a little mycelim growth. I see NOTHIN, like...at all. Is this normal? i thought at 5 days you should see at least SOMETHING. I really wanted to see a little growth/pick out the contams before i leave on vacation. Any thoughts would be helpful!
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scatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
Loc:
Last seen: 11 years, 1 month
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Re: I am scared (5 days after injection) [Re: PsyWonderCap]
#4489390 - 08/03/05 11:16 AM (18 years, 6 months ago) |
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You should normally see growth by then, but sometimes you dont for a number of reasons. Just be patient. The good news is, most molds would have shown up by now, if you contamed it with the syringe or during inoculations. They could still become contamed or have been sterilized poorly and get mold, and bacteria shows up anytime when things are to wet, but I wouldnt worry to much.
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hyphae
born to grow


Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 12 years, 9 months
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Re: I am scared (5 days after injection) [Re: scatmanrav]
#4489443 - 08/03/05 11:31 AM (18 years, 6 months ago) |
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Yes depending on the freshness of the spores it can take up to 14 days or so for germination to occur. Remember patience is a virtue!
-------------------- Getting the most out of your casings!, A pinning strategy. Oyster Shell "Flour" $2 for 1lb. a hell of a deal Not what is overlay but rather what overlay is Gas Exchange vs. FAE "We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"
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bwalker
The BodyElectric
Registered: 01/02/05
Posts: 63
Loc: NJ, USA
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Re: I am scared (5 days after injection) [Re: PsyWonderCap]
#4490022 - 08/03/05 01:43 PM (18 years, 6 months ago) |
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What's your incubation temperature? Try to keep it around 86 degrees, although lower than that is better than higher. So long as it hasn't been in the 90s-100s, they're most likely just slow to colonize.
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djsage420
aka DJQBNSIS


Registered: 02/15/05
Posts: 869
Loc: Panhandle
Last seen: 17 years, 11 months
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Re: I am scared (5 days after injection) [Re: bwalker]
#4490374 - 08/03/05 02:47 PM (18 years, 6 months ago) |
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I have been working with rye and rice for six months and now Im doing WBS because its cheap and easy, my first batch of jars didnt show any growth for over a week, now I can see it. This is what happend, the spores germinated in the middle of all my jars and not on the outside so I had to wait for the mycelium to poke through from the middle, you just have to have more patience, Ive heard of people on here waiting a month and they get growth, but to answer your question you will almost allways see growth in that time period.
-------------------- If you put your head to the grass, you can hear it growing
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PsyWonderCap
Stranger
Registered: 07/19/05
Posts: 73
Last seen: 18 years, 5 months
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Re: I am scared (5 days after injection) [Re: djsage420]
#4492948 - 08/03/05 11:23 PM (18 years, 6 months ago) |
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thanks guys....yea...cuz i dont think i hardcore shot it to the side of the glass..but yea i was excited not to find any contams...yet (just so i dont jinx myself :-P )
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FreedomFight
Strange

Registered: 07/03/05
Posts: 427
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Re: I am scared (5 days after injection) [Re: PsyWonderCap]
#4493271 - 08/04/05 12:10 AM (18 years, 6 months ago) |
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My friend saw some after 10 days, just barely. He is pretty excited as he thought they were dead. Keep waiting.
-------------------- I do not grow anything illegal. I do not sell anything. I am, however, a very curious individual. I also try to be helpful.
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droptopsunfire03
Wandering Deity

Registered: 08/04/05
Posts: 174
Last seen: 16 years, 2 months
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Re: I am scared (5 days after injection) [Re: FreedomFight]
#4493398 - 08/04/05 12:39 AM (18 years, 6 months ago) |
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Most of mine showed little spots of growth on the 5th day afyer 10 days or so, all were showing heavy growth, and im a newb so just wait man:)
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TrippingDuality
cult classic


Registered: 07/14/05
Posts: 805
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my first bag colonized super fast, but my second one has not shown any growth and it has been 7 days. be patient i know it's not easy. no sign of contams is a great sign.
-------------------- turn off your mind relax and float downstream
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