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Invisibletony8404
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inoculation q
    #4482358 - 08/01/05 08:59 PM (11 years, 4 months ago)

Okay, my 3 corn jars looked good when i got home so i took my glove box and sterilized it put the jars in and took my golden teacher syringe and heated the needle and cooled it with a squirt .. then i took the jar on a slant and inoculated through the polyfil in four different spots about 1cc each spot i did one in the center and three sides... i tried to get the needle against the glass but was not long enough so it hit corn/glass and rolled down.. then i put aluminum back on top and then i noticed the water from the spore solution on the bottom.. so i kinda turned the jar on a slant once or twice to make sure the spores are all over was that a good idea or do u just let the spore solution stay on the bottom?


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OfflineCLUTCH
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Re: inoculation q [Re: tony8404]
    #4482426 - 08/01/05 09:14 PM (11 years, 4 months ago)

Shouldn't matter either way, but it sounds like you used quite a bit of spore solution. Did you put 4 cc's in that one jar?

I hope it doesn't mess up your water content. You may be alright though.


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OfflineIGnosticAbhorI
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Re: inoculation q [Re: CLUTCH]
    #4482434 - 08/01/05 09:15 PM (11 years, 4 months ago)

I hope you soaked for 24 hours and then PC.

Otherwise you're looking at Germinating Endospores Taking Over and a waste of your time and supplies.

-Gnostic


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Invisibletony8404
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Re: inoculation q [Re: IGnosticAbhorI]
    #4482469 - 08/01/05 09:22 PM (11 years, 4 months ago)

oh no.. i soaked 24, then simmered , then strained for 45mins then i pc'ed them for 80mins psi 10, then let sit over night till i got home from work today at 6:30 ... then i took them out checked them and used 1cc x3 but my syringe was already at 8cc wasnt full 10cc... i think i used about 3cc to maybe 3.5cc plus a little for each time i change jars cause i reheated the needle and used alittle bit to cool.. so id say about 3cc to 3.5cc per jar is that too much?


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Invisibletony8404
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Re: inoculation q [Re: tony8404]
    #4482481 - 08/01/05 09:24 PM (11 years, 4 months ago)

i just shook them a little bit to make sure there all over that wouldnt be to much would it?


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OfflineCLUTCH
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Re: inoculation q [Re: tony8404]
    #4482501 - 08/01/05 09:30 PM (11 years, 4 months ago)

nah, should be ok.

Also, you can just sterilize the needle by flame the first time.

Then keep the needle in a soaked cotton ball with alcohol while switching to the other jar or hole. No need to sterilize the needle between each hole if you keep it in the cotton.

I started out flaming between each hole on pf cakes and it took forever when doing a bunch of jars. Then I read it wasn't necessary and stopped. It really cuts the time down.

EDIT:
As for the shaking. You did no harm. Some shake the hell out of it right after knocking them up. Everyone does it different.


Edited by CLUTCH (08/01/05 09:32 PM)


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OfflineIGnosticAbhorI
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Re: inoculation q [Re: CLUTCH]
    #4482656 - 08/01/05 10:14 PM (11 years, 4 months ago)

:thumbup:

Cept 3.5CC's is good enough for like 2 quarts and a pint lol...

-Gnostic


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InvisibleSubGen1us
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Re: inoculation q [Re: tony8404]
    #4482670 - 08/01/05 10:18 PM (11 years, 4 months ago)

If u have multiple jars u could experiment with each jar.
Wat i mean is put 1 cc in one and half cc in another or watever.
mark them.
That way u know wat amount works best with the method u used.
hope that makes sence.

its corn i dont need to squirt in multiple locations.
thats for like cakes that u cant shake.

Good luck.

[edit] guess u already did it so theres no turning back.
Hope everything turns out ok.


--------------------


Edited by SubGen1us (08/01/05 10:20 PM)


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Invisibletony8404
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Re: inoculation q [Re: SubGen1us]
    #4483445 - 08/02/05 12:58 AM (11 years, 4 months ago)

i hope it works.. got them in a box and in a closet at about 80...there blue meanies...hope they turn out.. i also finished pc'ing 3 more jars at 10 psi 1hr 10 minutes witt verm 1/2" on the bottom of the popcorn jar gonna take them out tommorro and use 1cc each jar of golden teacher i think or one other not sure yet


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OfflineIGnosticAbhorI
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Re: inoculation q [Re: tony8404]
    #4483517 - 08/02/05 01:08 AM (11 years, 4 months ago)

:handth:

-Gnostic


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