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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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Offlinebob_smith93456
Bad MotherChicken
Registered: 07/28/05
Posts: 188
Last seen: 12 years, 7 months
Newbie
    #4462607 - 07/28/05 02:21 AM (12 years, 10 months ago)

Hi, this is my first time so be gentle :smile:.

I've read through all the basic tek guides and have decided to go with the ultra sonic humidifier method in a plastic tub.  I'm still having problems getting my humidity down to 90% (I think that's where it's supposed to be) but I'm going to try and add a few bottles to see if it fixes it.

My real questions are as follows.  I see conflicting info on these questions in the guides so I figured I'd ask you guys how you do it.

1.  Should the tops be sealed on the jars and have the 4 holes the only oxygen source?
2.  How long can I wait after PC'ing the jars/substrate before inoculating them without running the risk of contamination?
3.  I would rather not do the glove box.  I would like to turn the a/c and fans off, clear a place on a table, wipe down the table with 95% ethanol, put 2 candles on the table, far enough away from my work space but close enough to kill any airborne bacteria, use (sterile) surgical gloves, run the needle through the flame, wait till it cools, then inoculate the jars.  Can you see anything wrong with this method?  Am I eliminating any contaminants without harming the spores?
4.  What temp should the jars incubate at?
5.  How much light should the jars receive during incubation?
6.  What temp/humidity should the cakes fruit at?
7.  How much light should they have during the fruiting process?  I have a 50 watt grow light, I guess I?ll need to put it a ways away from the tub?

Thanks!


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InvisibleFreedomFight
Strange

Registered: 07/03/05
Posts: 427
Re: Newbie [Re: bob_smith93456]
    #4462662 - 07/28/05 02:29 AM (12 years, 10 months ago)

1. Yes, that works fine
2. Assuming you PCed them correctly and you leave the aluminum foil on, they should last a very long time, I read a post where someone colonized a year old sterile jar.
3. I personally don't see how the candles will help. I would clean the table with soap/water then low conc. bleach but I am bleach crazy.
4. around 82F I believe.
5. No light would be the most optimal, although checking on them once in a while doesn't hurt.
6. 75F or so, 90% RH I think.
7. Light is not really a problem. Just try to keep it coming from the top so the shrooms don't gnarl.


--------------------
I do not grow anything illegal.
I do not sell anything.
I am, however, a very curious individual.
I also try to be helpful.


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Invisiblememes
Blessed

Folding@home Statistics
Registered: 01/11/05
Posts: 27,748
Loc: In a Tree
Re: Newbie [Re: bob_smith93456]
    #4462675 - 07/28/05 02:31 AM (12 years, 10 months ago)

a


Edited by memes (10/17/12 06:54 PM)


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Offlinebob_smith93456
Bad MotherChicken
Registered: 07/28/05
Posts: 188
Last seen: 12 years, 7 months
Re: Newbie [Re: memes]
    #4478263 - 07/31/05 09:03 PM (12 years, 10 months ago)

Thanks for the help guys!


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