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Registered: 10/23/01
Posts: 55
Last seen: 22 years, 1 month
Making honey water for syringes
    #445566 - 11/03/01 10:27 AM (22 years, 1 month ago)

Do i just boil the honey in the water im sterilizing for the syringe? how much should i toss in?

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-H.S. Thompson

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Carpal Tunnel

Folding@home Statistics
Registered: 04/23/00
Posts: 5,015
Re: Making honey water for syringes [Re: MycoDorkus]
    #445570 - 11/03/01 10:29 AM (22 years, 1 month ago)

I used this tek with a little success. I defently had colonization but I never took it to jars. It is best to use unpasturized honey:


1/2 pint wide mouth jar
bottled water
1 tsp honey
saran wrap
rubber band
masking tape
microwave oven
2 cup microwave safe measuring cup
Sterile spore syringe
Disinfectant such as Lysol or bleach water in a spray bottle
Pot holder or oven mitt


Have all materials at hand so you can work quickly.
Put about 1 1/8 cups water in the measuring cup.
Add about 1 tsp honey to the water and stir until dissolved.
Place the cup and the half-pint jar in the microwave.
Fry on high for five minutes
Open the microwave do not remove the cup and jar.
Working quickly within the microwave and using the potholder to protect your hand, pour water into the jar to about the bottom of the threads. Stretch saran wrap over the top of the jar and secure with a rubber band.
Let cool to room temperature.
Clean a working surface such as a table with a disinfectant such as Lysol or bleach water.
Mist the surrounding air with the disinfectant.
Working quickly inoculate the jar through the saran wrap with a sterile spore syringe using about 2 cc and cover hole with masking tape.
Incubate at 80 degrees Fahrenheit.
Once the mycelium has grown, shake to distribute the mycelium, and poke thru the saran wrap with a sterile syringe and suck up the white mycelium, cover the hole with tape and save for further use.

"There's only one corner of the universe you can be certain of improving and that's your own self. So you have to begin there, not
outside, not on other people" - Aldous Huxley

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myco contractor
Registered: 09/19/01
Posts: 2,417
Re: Making honey water for syringes [Re: MycoDorkus]
    #445579 - 11/03/01 10:42 AM (22 years, 1 month ago)

I havent had a problem yet I took a half pint jar and put in a little over a tbsp of honey then filled the jar up almost to the top. Pc for 30min(probably to much a little carmalizing) . The lid on the jar had a hole in it with tape over it. I got another piece of tape ready and quicklyinjected half a syringe 6cc into it careful not to let the needle touch the water. after aboout a week I had growth and in about 2 1/2 I had colonized honey water.

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