Home | Community | Message Board
You are not signed in.
Sign In New Account
Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery


This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Advanced Mycology
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

JK Botanik Shop: Jumbo Size Kratom Capsules, Kratom Variety Bundles

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Invisiblealtarego
member
Registered: 10/25/01
Posts: 130
Glass/reusable petris: Anyone use them?
    #444842 - 11/02/01 02:31 PM (21 years, 10 months ago)

Does anyone out there have experience using reusable petri dishes, either glass or ppe? I have a few questions about thier use.

1. Do you pour them before or after sterilization?

2. If before, how do you keep them sterile after autoclaving? (I thought about using a FP bag, heat sealed.)

3. If after, how do you pre-sterilize the empty plates? (I thought about doing the same trick as above, leaving them in the bag until ready to use.)

My first attempt was pre-pouring, placing them in an FP bag and heat sealing, sterilizing for 20 min at 15psi, and cooling. Unfortunately they either took on water or the gel strength of the agar had died, since they were watery.

Anyone have any good advice on this one?

Thanks
AE


--------------------
Support Cognitive Liberty! Join Alchemind!
Support psychedelic research! Join MAPS!


Extras: Filter Print Post Top
OfflineAzure
old hand
Registered: 12/31/98
Posts: 469
Loc: California, USA
Last seen: 21 years, 4 months
Re: Glass/reusable petris: Anyone use them? [Re: altarego]
    #445558 - 11/03/01 10:20 AM (21 years, 10 months ago)

If you pour the agar medium into the petri dishes before sterilizing, you lower the potential for contamination. The only downside is that you have to pre-melt the nutrified agar solution, pour it into the dishes, and allow it to solidify before pressure cooking.


Extras: Filter Print Post Top
Invisiblealtarego
member
Registered: 10/25/01
Posts: 130
Re: Glass/reusable petris: Anyone use them? [Re: Azure]
    #446780 - 11/04/01 03:46 PM (21 years, 10 months ago)

Well, that's what I did, with mixed results. I put the poured plates (20) in a FP bag, sealed it, placed it in my pc and gave it 45 min @ 15psi.

They came out fine except for two things:

1. There was lots of condensation, more than I'd like (the covers are hard to see thru and the condensation drips onto the agar.)

2. Several plates on the top of the stack fell off in the cooking, and dumped their contents, forcing me to toss them and all the ones on the bottom layer. Some way to stack them securely would be good, but I haven't figured that one out yet.

Anyone with any further thoughts?

Thanks mucho
AE


--------------------
Support Cognitive Liberty! Join Alchemind!
Support psychedelic research! Join MAPS!


Extras: Filter Print Post Top
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/17/99
Posts: 24,162
Loc: my room
Last seen: 2 days, 12 hours
Re: Glass/reusable petris: Anyone use them? [Re: altarego]
    #446881 - 11/04/01 05:36 PM (21 years, 10 months ago)

Stack the plates not more than 5 piece high, then put onto a piece of aluminium foil and fold the foil around them. Place in the PC on a rack and very little water. PC for 20 minutes(40 minutes is definitely an overkill). Let cool in the PC. You will always have condensations if you srtilize allready filled petris.


--------------------
Grow Mushrooms from Spores - PF-Tek for Simple Minds


Extras: Filter Print Post Top
Jump to top Pages: 1

JK Botanik Shop: Jumbo Size Kratom Capsules, Kratom Variety Bundles

Mushrooms, Mycology and Psychedelics > Advanced Mycology
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Similar ThreadsPosterViewsRepliesLast post
* Petri dishes and agar MyOwnReality 4,347 18 03/01/08 08:12 PM
by MycoAu
* Sterilising petri dishes? Peace_Patrol 3,629 18 08/20/02 04:37 PM
by Alkaloids
* Re: Hello, can I sterilize media filled glass petris in the microwave? Anonymous 1,962 9 02/08/00 07:35 AM
by Anonymous
* Plastic Petris'...am i screwed?
( 1 2 all )		 Azmodeus 2,880 20 02/07/03 09:35 PM
by stonErollEr1
* petris getting wet! pleezr 1,314 5 01/22/02 12:11 PM
by pleezr
* Excessive water in Petri Dishes
( 1 2 all )		 TRIPPYCHIK 12,769 29 02/03/11 04:59 AM
by RogerRabbit
* Where can i find Glass Petri dishes?..anyone? Numba9 1,569 6 09/02/01 02:38 AM
by oscill8
* Pyrex petri dishes imperfect rims? Shaw 1,261 1 07/14/04 12:58 PM
by Anno

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, Pastywhyte, bodhisatta
1,075 topic views. 1 members, 3 guests and 1 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2023 Mind Media. Some rights reserved.

Generated in 0.023 seconds spending 0.005 seconds on 12 queries.