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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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OfflinePhake_ld
enthusiast
Registered: 10/08/01
Posts: 230
Last seen: 22 years, 4 months
Sterilization Question
    #443692 - 10/31/01 09:58 PM (23 years, 1 month ago)

Will soaking my jars in Pine_Sol Cleaner & Antibacterial solution prior to adding substrate to them be a good sterilization technique?

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Offlinegusb232
member

Registered: 05/01/01
Posts: 162
Loc: pm me
Last seen: 22 years, 9 months
Re: Sterilization Question [Re: Phake_ld]
    #443697 - 10/31/01 10:03 PM (23 years, 1 month ago)

It might be ok if you rinse well afterwards you will want to dilute the pine sol first also, I know with bleach a 10% solution is recomended. Why dont you just use bleach.
Actully I dont think it is necessisary if you are going to PC the jar though.

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Invisiblephrozendata
Carpal Tunnel

Folding@home Statistics
Registered: 04/23/00
Posts: 5,015
Re: Sterilization Question [Re: gusb232]
    #443716 - 10/31/01 10:25 PM (23 years, 1 month ago)

Indeed, a good soak in 10% bleach is highly advised. You shouldnt leave submerged for much longer than 12 hours or else you risk a residue buildup to create on the jars. A good six hours is advised. You'll have to wash the jars out with hot water and soap after too. I would also advise to dry them with a paper towel or clean cloth. You just spent all that time getting them rather sterile and you certanily dont want to rub a bunch of contaminates on them.


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"There's only one corner of the universe you can be certain of improving and that's your own self. So you have to begin there, not
outside, not on other people" - Aldous Huxley

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OfflineOsh_Kosh_BGosh
journeyman

Registered: 10/09/01
Posts: 79
Last seen: 23 years, 17 days
Re: Sterilization Question [Re: phrozendata]
    #443717 - 10/31/01 10:26 PM (23 years, 1 month ago)

blah blah blah... pc'ing or boiling them is good enough

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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