Sterilization Question - Mushroom Cultivation

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Phake_ld
enthusiast

Registered: 10/08/01
Posts: 230
Last seen: 21 years, 4 months

Sterilization Question

#443692 - 10/31/01 09:58 PM (22 years, 1 month ago)

Will soaking my jars in Pine_Sol Cleaner & Antibacterial solution prior to adding substrate to them be a good sterilization technique?

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gusb232
member

Registered: 05/01/01
Posts: 162
Loc: pm me
Last seen: 21 years, 9 months

Re: Sterilization Question

[Re: Phake_ld]
#443697 - 10/31/01 10:03 PM (22 years, 1 month ago)

It might be ok if you rinse well afterwards you will want to dilute the pine sol first also, I know with bleach a 10% solution is recomended. Why dont you just use bleach.
Actully I dont think it is necessisary if you are going to PC the jar though.

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phrozendata
Carpal Tunnel

Registered: 04/23/00
Posts: 5,015

Re: Sterilization Question

[Re: gusb232]
#443716 - 10/31/01 10:25 PM (22 years, 1 month ago)

Indeed, a good soak in 10% bleach is highly advised. You shouldnt leave submerged for much longer than 12 hours or else you risk a residue buildup to create on the jars. A good six hours is advised. You'll have to wash the jars out with hot water and soap after too. I would also advise to dry them with a paper towel or clean cloth. You just spent all that time getting them rather sterile and you certanily dont want to rub a bunch of contaminates on them.

--------------------
"There's only one corner of the universe you can be certain of improving and that's your own self. So you have to begin there, not
outside, not on other people" - Aldous Huxley

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Osh_Kosh_BGosh
journeyman

Registered: 10/09/01
Posts: 79
Last seen: 22 years, 13 days

Re: Sterilization Question

[Re: phrozendata]
#443717 - 10/31/01 10:26 PM (22 years, 1 month ago)

blah blah blah... pc'ing or boiling them is good enough

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation

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