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Invisibledurban_poison
myco contractor
Registered: 09/19/01
Posts: 2,415
honey water
    #443372 - 10/31/01 06:30 PM (15 years, 1 month ago)

so far I have been successful at making honey water with multi spore innoculation. my question is if i want to clone a mushroom can I just put a sterile chunk of a stem in it to make more honey water?


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Offlineshitdog
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Registered: 10/24/01
Posts: 132
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Last seen: 14 years, 10 months
Re: honey water [Re: durban_poison]
    #443382 - 10/31/01 06:40 PM (15 years, 1 month ago)

What is honey water?


--------------------
I'll never get out of this maze


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Invisiblemrshrums
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Registered: 08/04/01
Posts: 147
Re: honey water [Re: durban_poison]
    #443387 - 10/31/01 06:43 PM (15 years, 1 month ago)

I believe there is a way to clone from the core of a mushrooms stem but I doubt doubt you can just
put it in honey water and grow mycillium from it.


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Invisibledurban_poison
myco contractor
Registered: 09/19/01
Posts: 2,415
Re: honey water [Re: durban_poison]
    #443401 - 10/31/01 07:01 PM (15 years, 1 month ago)

why not its the same principle as agar?


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Invisiblemrshrums
member
Registered: 08/04/01
Posts: 147
Re: honey water [Re: durban_poison]
    #443405 - 10/31/01 07:04 PM (15 years, 1 month ago)

LIke I said I doubt it will work I've never done it before so I don't know.


Edited by mrshrums (10/31/01 07:05 PM)


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Invisibledurban_poison
myco contractor
Registered: 09/19/01
Posts: 2,415
Re: honey water [Re: durban_poison]
    #443854 - 10/31/01 09:49 PM (15 years, 1 month ago)

somebody has to know?


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Offlinesylo
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Registered: 10/30/00
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Re: honey water [Re: durban_poison]
    #444173 - 11/01/01 02:29 AM (15 years, 1 month ago)

Yes, it can be done. Hippie has written about it here and you might also check Mycotopia for the tek. Sterility is absolutely essential and the surface of the stem is surely contaminated. The stem must be asepticly cut and then peeled like a banana. It is not easy but it can be done.


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Offlinefelixhigh
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Registered: 06/24/01
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Re: honey water [Re: durban_poison]
    #444191 - 11/01/01 02:42 AM (15 years, 1 month ago)

hmmm! my wookie friend has already done that. he putted little pieces of inner stripes from a mushrrm and innoculated substrate with successful growth... the only problem he had was bcs he didnt wait for more myceial growth in honey, so colonization was slow. and he had problems with contamination into honeywater, he had those wonderful clouds and a black spot on the side... he is thinking of upgrading to agar teks...


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Invisiblepuscle
genius of love
Registered: 01/07/01
Posts: 4,539
Loc: NY
Re: honey water [Re: durban_poison]
    #444407 - 11/01/01 07:22 AM (15 years, 1 month ago)

Yes, it can be done. But I've never gotten it to grow contam free.


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