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I have been told that alkaloid levels in submerged (liquid) mycelium cultures are negligible... however, there are guides such as The Psilocybin Producer's Guide which detail such techniques and claim they are effective. Does anyone have any experienced with submerged mycelium culture and can anyone confirm this?
Alkakoid levels in submerged cultures are nearly the same as fruiting bodies. The yield for submerged culture is poor. You can get a much better yield if you add some tooth picks or flakes of styrofoam to the medium and let the mycelium grow as a skin on the surface. Do not shake the medium. The surface harvest can be hung up to dry and looks like water stained sheets of paper. The taste is actually quite good so forget about extraction. The alkaloid does not leak out of healthy cells so there is nothing in the liquid.
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