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Registered: 04/27/05
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a sterile inoculation idea..
    #4425285 - 07/19/05 10:08 PM (11 years, 3 months ago)

Im trying to put together an inoc method that will be fairly easy and have a high level of safety/cleanliness-

agar (i think) had recently posted a method involving a cotton swab etc-
but I thought maybe I could trim it up?

heres what I had in mind

take the jars out of the pot or pc and place fresh alcohol pads over the holes

and cling wrap over the entire thing

flame and wipe if needed and inject through the wrap and through the pad--

any suggestions??

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Trippin' fool -Merry Prankster

Registered: 03/12/05
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Re: a sterile inoculation idea.. [Re: cloudcity]
    #4425373 - 07/19/05 10:33 PM (11 years, 3 months ago)

I had thought of using the polyfill/tyvek wbs tek and soaking the polyfil pre-inoculation with a small ammount of alcohol before injecting.

Through the excercise of patience, one may learn humility..

Smoke plenty of green, and eat fungus!!!!

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Registered: 07/07/05
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Loc: Tampa
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Re: a sterile inoculation idea.. [Re: Smallworlds]
    #4425463 - 07/19/05 10:58 PM (11 years, 3 months ago)

sounds like a great idea with the alcohol pads. ill have to snatch some from work.

Living in peace, outside myself.

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Re: a sterile inoculation idea.. [Re: prosthetics]
    #4425516 - 07/19/05 11:10 PM (11 years, 3 months ago)

Why not get thease bad boys
they are self healing works like a charm:)

If you put your head to the grass, you can hear it growing

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fury 762 where are you?

Registered: 03/21/05
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Re: a sterile inoculation idea.. [Re: prosthetics]
    #4425572 - 07/19/05 11:20 PM (11 years, 3 months ago)

I think it was Magash that had a pretty fool proof inoculation method .....

Get a super fine pore sponge (like the makup ones) bran new and cut it to about 1/4" longer than the needle. Then soak some alchohol in it then wring it out fairly well. Flame sterilize the needle then stick the sponge all the way on the needle.

Then when you go to inoculate a jar simply push the needle through the sponge into the jar, then when you retract the needle from the jar the sponge will cover the needle again .

Its just like swabbin the needle after every innoc hole without ever having to actually swab it. It does it its self. :stoned:

Take care n be safe....

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The Mother Fucking Bear-o-dactyl
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Re: a sterile inoculation idea.. [Re: cloudcity]
    #4425755 - 07/19/05 11:50 PM (11 years, 3 months ago)

Don't make it any harder than it needs to be. After your jars are cool, just alcohol swab the tape, probably not needed since its sterile, but its a good idea in case anything fell on them in the short time its been in open air. Inoculate through the tape, get a band aid ready, and cover the hole with a band aid. If you get your technique down the injection hole will never see open air. What you do is get the band aid ready to go by peeling the plastic covers over, set it down on a sterile surface. Then alcohol swab the tape. Pick up the syringe with your right hand and the band aid with your left, inject the spores by puncturing the tape with the needle, and before you remove the needle get the band aid started going over the hole. In a swift move, remove the needle, and seal the band aid.

There's a thousand ways to do it, find one that works for you.

Electrical tape is best for this, when you send a needle through it it makes an air tight seal around the needle.  :thumbup:


[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]

Edited by Psychoslut (07/19/05 11:58 PM)

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