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Smallworlds
Trippin' fool -Merry Prankster
Registered: 03/12/05
Posts: 4,207
Last seen: 18 years, 5 months
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Failed LC jar
#4423635 - 07/19/05 01:13 PM (18 years, 8 months ago) |
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Yup I inoculated with my semi-colonized 45 day old LC jar (which had been stalled for a couple of weeks, but I thought what the hell), and I have zero growth in 4 days, while the others I did from spores are already showing good growth in the 4 days.
Since LC is supposed to shave time off of germination time and what not, (and therefore should have been the first to germinate) I assumed my LC jar was dead and chucked it.
Possible reasons include that I placed a nickel in the jar instead of the recommended BB, and possibly it fouled the karo-water. It looks oxidized and is a gray color now.
Could that be it, or should I look elsewhere for the reason?
-------------------- Through the excercise of patience, one may learn humility.. Smoke plenty of green, and eat fungus!!!! Trip Report
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coda
Banjo Goiter
Registered: 03/20/01
Posts: 8,750
Last seen: 1 year, 8 days
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man i feel you, my LC's have been doing pretty shitty. My B+ seems to have stopped growing and my TC and GT seem to be doing not much either. I dunno, but the nickel may have had something to do with it esp if you didnt PC it.
-------------------- To get really high is to forget yourself. And to forget yourself is to see everything else. And to see everything else is to become an understanding molecule in evolution, a conscious tool of the universe. And I think every human being should be a conscious tool of the universe. . . . -JG i really am glad you came back to us instead of taking the other path. *hug* -A_S (RIP your final words to me will never be forgotten) Don't fuck with the laughing jesus.
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Smallworlds
Trippin' fool -Merry Prankster
Registered: 03/12/05
Posts: 4,207
Last seen: 18 years, 5 months
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Re: Failed LC jar [Re: coda]
#4423895 - 07/19/05 02:27 PM (18 years, 8 months ago) |
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Well, I did PC it. For 10 or 15 minutes, I forget. Oh well, I'll try that again.. This time with a damn BB or a piece of broken glass. Something non-reactive.
-------------------- Through the excercise of patience, one may learn humility.. Smoke plenty of green, and eat fungus!!!! Trip Report
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Roadkill
Retired Shroomery Mod
Registered: 12/11/01
Posts: 22,674
Loc: Montana
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use a glass marble.
what kind of LC was it?
recipy?
I don't think using the nickel was a good idea.
tc
-------------------- Laterz, Road Who the hell you callin crazy? You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch! Brainiac said: PM the names with on there names, that means they have mushrooms for sale.
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Smallworlds
Trippin' fool -Merry Prankster
Registered: 03/12/05
Posts: 4,207
Last seen: 18 years, 5 months
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Re: Failed LC jar [Re: Roadkill]
#4423969 - 07/19/05 02:44 PM (18 years, 8 months ago) |
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It was a 2 TBS karo, 1/2 pint water, PC'd for 10 or 15 min, then in a glove box the water was poured into a 100% colonized WBS jar (as much as would fit), top replaced then shaken, then re-opened and poured back into the original LC jar.
I used the same tyvek/polyfill lid style jar as I usually use for WBS. The thing actually took off for a while, and there was growth a bit less than the size of a golfball or so in it. Then the mycelium started looking darker than I thought it should look, and it simultaneously stalled a couple weeks ago.
I took a chance on it and ended up dumping a case of jars in the toilet. lol
Luckily I didn't trust that jar and so I did some with spores too.
-------------------- Through the excercise of patience, one may learn humility.. Smoke plenty of green, and eat fungus!!!! Trip Report
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Roadkill
Retired Shroomery Mod
Registered: 12/11/01
Posts: 22,674
Loc: Montana
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too much karo...IMHO
try using...
4cc of Karo 96cc of water 1 glass marble
***double up the recipy for larger jars***
pc for 20 minutes at 15 psi.
cool it down.
inject 1 to 2 cc of a spore syringe...and stirr it up.
put into your incubator at 81 degrees F.
comes out looking like....
tc
-------------------- Laterz, Road Who the hell you callin crazy? You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch! Brainiac said: PM the names with on there names, that means they have mushrooms for sale.
Edited by Roadkill (07/19/05 02:55 PM)
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Smallworlds
Trippin' fool -Merry Prankster
Registered: 03/12/05
Posts: 4,207
Last seen: 18 years, 5 months
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Re: Failed LC jar [Re: Roadkill]
#4424024 - 07/19/05 02:55 PM (18 years, 8 months ago) |
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Thank you, will try next time using your recepie!!
-------------------- Through the excercise of patience, one may learn humility.. Smoke plenty of green, and eat fungus!!!! Trip Report
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Smallworlds
Trippin' fool -Merry Prankster
Registered: 03/12/05
Posts: 4,207
Last seen: 18 years, 5 months
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Re: Failed LC jar [Re: Roadkill]
#4424040 - 07/19/05 02:59 PM (18 years, 8 months ago) |
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Another thing, my jar was the color of urine even though I used light syrup. I imagine it carmelized??
Your jar looks clear. Did it look yellowish before it colonized?
-------------------- Through the excercise of patience, one may learn humility.. Smoke plenty of green, and eat fungus!!!! Trip Report
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Roadkill
Retired Shroomery Mod
Registered: 12/11/01
Posts: 22,674
Loc: Montana
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no it was clear... I use karo light too.
sounds like your's carmelized.
tc
-------------------- Laterz, Road Who the hell you callin crazy? You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch! Brainiac said: PM the names with on there names, that means they have mushrooms for sale.
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Smallworlds
Trippin' fool -Merry Prankster
Registered: 03/12/05
Posts: 4,207
Last seen: 18 years, 5 months
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Re: Failed LC jar [Re: Roadkill]
#4424074 - 07/19/05 03:09 PM (18 years, 8 months ago) |
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How do you prevent that? Was it because I used too much Karo?
-------------------- Through the excercise of patience, one may learn humility.. Smoke plenty of green, and eat fungus!!!! Trip Report
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Roadkill
Retired Shroomery Mod
Registered: 12/11/01
Posts: 22,674
Loc: Montana
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could be several reasons.
too much karo...
or you didn't mix up the karo and water good enough before pc'ing.
---
Next time use warm water with the karo.
and stirr it up good in the jar...before pc'ing it.
thats what I do.
-------------------- Laterz, Road Who the hell you callin crazy? You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch! Brainiac said: PM the names with on there names, that means they have mushrooms for sale.
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Smallworlds
Trippin' fool -Merry Prankster
Registered: 03/12/05
Posts: 4,207
Last seen: 18 years, 5 months
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Re: Failed LC jar [Re: Roadkill]
#4424146 - 07/19/05 03:21 PM (18 years, 8 months ago) |
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Sweet, thanks!!
-------------------- Through the excercise of patience, one may learn humility.. Smoke plenty of green, and eat fungus!!!! Trip Report
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optic
Stranger
Registered: 06/01/05
Posts: 53
Last seen: 18 years, 5 months
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I always have trouble with spore syringes stalling... However, since I switched to breaking off a small part of a cake and putting it in the LC mix, I have had great results within 3 days. Just make sure you are in a clean place when you do it... much better than waiting for spores to do their thing.
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Smallworlds
Trippin' fool -Merry Prankster
Registered: 03/12/05
Posts: 4,207
Last seen: 18 years, 5 months
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Re: Failed LC jar [Re: optic]
#4424206 - 07/19/05 03:40 PM (18 years, 8 months ago) |
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The bits of cake could possibly clog the syringe needle though, right?
-------------------- Through the excercise of patience, one may learn humility.. Smoke plenty of green, and eat fungus!!!! Trip Report
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