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Amazon Shop: ½ Pint Jars, Wild Bird Seed

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OfflineSmallworlds
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Registered: 03/12/05
Posts: 4,207
Last seen: 11 years, 10 months
Failed LC jar
    #4423635 - 07/19/05 03:13 PM (12 years, 1 month ago)

Yup I inoculated with my semi-colonized 45 day old LC jar (which had been stalled for a couple of weeks, but I thought what the hell), and I have zero growth in 4 days, while the others I did from spores are already showing good growth in the 4 days.

Since LC is supposed to shave time off of germination time and what not, (and therefore should have been the first to germinate) I assumed my LC jar was dead and chucked it.

Possible reasons include that I placed a nickel in the jar instead of the recommended BB, and possibly it fouled the karo-water. It looks oxidized and is a gray color now.

Could that be it, or should I look elsewhere for the reason?


--------------------
Through the excercise of patience, one may learn humility..

Smoke plenty of green, and eat fungus!!!!
:peace::heart::slomo::gd_icon::gd_icon::gd_icon::slomo:


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Offlinecoda
Banjo Goiter
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Registered: 03/20/01
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Re: Failed LC jar [Re: Smallworlds]
    #4423849 - 07/19/05 04:12 PM (12 years, 1 month ago)

man i feel you, my LC's have been doing pretty shitty. My B+ seems to have stopped growing and my TC and GT seem to be doing not much either. I dunno, but the nickel may have had something to do with it esp if you didnt PC it.


--------------------
To get really high is to forget yourself. And to forget yourself is to see everything else. And to see everything else is to become an understanding molecule in evolution, a conscious tool of the universe. And I think every human being should be a conscious tool of the universe. . . .

-JG



Don't fuck with the laughing jesus.


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OfflineSmallworlds
Trippin' fool -Merry Prankster

Registered: 03/12/05
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Re: Failed LC jar [Re: coda]
    #4423895 - 07/19/05 04:27 PM (12 years, 1 month ago)

Well, I did PC it. For 10 or 15 minutes, I forget. Oh well, I'll try that again.. This time with a damn BB or a piece of broken glass. Something non-reactive.


--------------------
Through the excercise of patience, one may learn humility..

Smoke plenty of green, and eat fungus!!!!
:peace::heart::slomo::gd_icon::gd_icon::gd_icon::slomo:


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InvisibleRoadkill
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Registered: 12/11/01
Posts: 22,598
Loc: Snoqualmie, Wa.
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Re: Failed LC jar [Re: Smallworlds]
    #4423935 - 07/19/05 04:36 PM (12 years, 1 month ago)

use a glass marble.

what kind of LC was it?

recipy?

I don't think using the nickel was a good idea.

tc


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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OfflineSmallworlds
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Registered: 03/12/05
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Re: Failed LC jar [Re: Roadkill]
    #4423969 - 07/19/05 04:44 PM (12 years, 1 month ago)

It was a 2 TBS karo, 1/2 pint water, PC'd for 10 or 15 min, then in a glove box the water was poured into a 100% colonized WBS jar (as much as would fit), top replaced then shaken, then re-opened and poured back into the original LC jar.

I used the same tyvek/polyfill lid style jar as I usually use for WBS. The thing actually took off for a while, and there was growth a bit less than the size of a golfball or so in it. Then the mycelium started looking darker than I thought it should look, and it simultaneously stalled a couple weeks ago.

I took a chance on it and ended up dumping a case of jars in the toilet. lol

Luckily I didn't trust that jar and so I did some with spores too.


--------------------
Through the excercise of patience, one may learn humility..

Smoke plenty of green, and eat fungus!!!!
:peace::heart::slomo::gd_icon::gd_icon::gd_icon::slomo:


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InvisibleRoadkill
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Re: Failed LC jar [Re: Smallworlds]
    #4424019 - 07/19/05 04:54 PM (12 years, 1 month ago)

too much karo...IMHO

try using...

4cc of Karo
96cc of water
1 glass marble

***double up the recipy for larger jars***

pc for 20 minutes at 15 psi.

cool it down.

inject 1 to 2 cc of a spore syringe...and stirr it up.

put into your incubator at 81 degrees F.

comes out looking like....



tc


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



Edited by Roadkill (07/19/05 04:55 PM)


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OfflineSmallworlds
Trippin' fool -Merry Prankster

Registered: 03/12/05
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Re: Failed LC jar [Re: Roadkill]
    #4424024 - 07/19/05 04:55 PM (12 years, 1 month ago)

Thank you, will try next time using your recepie!!


--------------------
Through the excercise of patience, one may learn humility..

Smoke plenty of green, and eat fungus!!!!
:peace::heart::slomo::gd_icon::gd_icon::gd_icon::slomo:


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OfflineSmallworlds
Trippin' fool -Merry Prankster

Registered: 03/12/05
Posts: 4,207
Last seen: 11 years, 10 months
Re: Failed LC jar [Re: Roadkill]
    #4424040 - 07/19/05 04:59 PM (12 years, 1 month ago)

Another thing, my jar was the color of urine even though I used light syrup. I imagine it carmelized??

Your jar looks clear. Did it look yellowish before it colonized?


--------------------
Through the excercise of patience, one may learn humility..

Smoke plenty of green, and eat fungus!!!!
:peace::heart::slomo::gd_icon::gd_icon::gd_icon::slomo:


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InvisibleRoadkill
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Loc: Snoqualmie, Wa.
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Re: Failed LC jar [Re: Smallworlds]
    #4424058 - 07/19/05 05:04 PM (12 years, 1 month ago)

no it was clear...
I use karo light too.

sounds like your's carmelized.

tc


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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OfflineSmallworlds
Trippin' fool -Merry Prankster

Registered: 03/12/05
Posts: 4,207
Last seen: 11 years, 10 months
Re: Failed LC jar [Re: Roadkill]
    #4424074 - 07/19/05 05:09 PM (12 years, 1 month ago)

How do you prevent that? Was it because I used too much Karo?


--------------------
Through the excercise of patience, one may learn humility..

Smoke plenty of green, and eat fungus!!!!
:peace::heart::slomo::gd_icon::gd_icon::gd_icon::slomo:


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InvisibleRoadkill
Retired Shroomery Mod
Male User Gallery

Registered: 12/11/01
Posts: 22,598
Loc: Snoqualmie, Wa.
Trusted Cultivator
Re: Failed LC jar [Re: Smallworlds]
    #4424101 - 07/19/05 05:13 PM (12 years, 1 month ago)

could be several reasons.

too much karo...

or you didn't mix up the karo and water good enough before pc'ing.

---

Next time use warm water with the karo.

and stirr it up good in the jar...before pc'ing it.

thats what I do.


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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OfflineSmallworlds
Trippin' fool -Merry Prankster

Registered: 03/12/05
Posts: 4,207
Last seen: 11 years, 10 months
Re: Failed LC jar [Re: Roadkill]
    #4424146 - 07/19/05 05:21 PM (12 years, 1 month ago)

Sweet, thanks!!


--------------------
Through the excercise of patience, one may learn humility..

Smoke plenty of green, and eat fungus!!!!
:peace::heart::slomo::gd_icon::gd_icon::gd_icon::slomo:


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Offlineoptic
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Registered: 06/02/05
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Last seen: 11 years, 10 months
Re: Failed LC jar [Re: Smallworlds]
    #4424192 - 07/19/05 05:35 PM (12 years, 1 month ago)

I always have trouble with spore syringes stalling... However, since I switched to breaking off a small part of a cake and putting it in the LC mix, I have had great results within 3 days. Just make sure you are in a clean place when you do it... much better than waiting for spores to do their thing.


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OfflineSmallworlds
Trippin' fool -Merry Prankster

Registered: 03/12/05
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Last seen: 11 years, 10 months
Re: Failed LC jar [Re: optic]
    #4424206 - 07/19/05 05:40 PM (12 years, 1 month ago)

The bits of cake could possibly clog the syringe needle though, right?


--------------------
Through the excercise of patience, one may learn humility..

Smoke plenty of green, and eat fungus!!!!
:peace::heart::slomo::gd_icon::gd_icon::gd_icon::slomo:


Trip Report


Post Extras: Print Post  Remind Me! Notify Moderator
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Amazon Shop: ½ Pint Jars, Wild Bird Seed

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