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RobMarley420
LSD Enthusiast
Registered: 05/01/05
Posts: 12,554
Loc: Mushroom Mountain
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How do I make sure my syringe 100% sterile?
#4422054 - 07/19/05 02:33 AM (18 years, 7 months ago) |
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I need to re-inocculate 5 jars and I need to make sure that my needle and syringe is 100% sterile. I inoculated 3 golden teacher jars and 5 B+ jars. I had some Golden Teacher spore solution left over in a syringe from my last grow. It was enough to knoc' up 3 jars. I sterilized the needle with an alchohol flame for about 15 secs, then wiped it off with hydrogen peroxide. All 3 of those jars got contamanated! I knoc'ed up 5 jars with B+ from a brand new syringe, the package was never opened. Never touched the needle or attempted to sterilize. All 5 of those jars show no signs of contams! But they are going slow as fuck compared to GT. So I'm 100% sure that the Golden Teachers were contamed becuase of the reused syringe, not because of my sterilization proccess (pressure cooking and inoculation environment). All of my B+ jars are going SLOW. 3 show tiny signs of growth but only at 1 injections site, the other 2 show nothing at all. I'm going to re-inject all of these jars to get some mycelium growing at all 4 injection sites on all 4 jars. I need to know how to sterilze my needle to make sure it won't contam my precious cakes. I sterilized my GT syringe with an alcohol flame and then wiped it down with h2o2. There was still a little bit of air in the syringe that was pushed out before i inoculated. Is this where the contams came from? Any help would be great!
These or my B+ jars
These pics were taken 8 days after inoculation.
Here on 2 of the 3 contamed GT jars
I ordered my spores from spores101.com. My GT syringes have about 10 times more sprores than my B+. The GT syringes have huge clumps of spores but one of my B+ syringes has a very small clump and the other has barely any at all! Is this the reason why the Golden Teachers were taking off and the B+ are so slow? And also on my last grow all of my Golden TEacher jars grew from all 4 injection sites, on this grow my B+ are only growing form 1 site (if any) Is this cause the syringe didn't have much spores in it? Why did they hook it up so SHADY on the B+ syringes?
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Edited by RobMarley420 (07/19/05 02:36 AM)
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BenKenobi
Enlightened 1
Registered: 05/22/05
Posts: 342
Loc: Europe
Last seen: 10 years, 10 months
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Re: How do I make sure my syringe 100% sterile? [Re: RobMarley420]
#4422338 - 07/19/05 03:55 AM (18 years, 7 months ago) |
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Ive reused syringes b4 no contam as yet. I just give the syringe an initial squeeze to get what is in the needle out then i flame it for 5sec with a BIC lighter and give it a good rub with a soaked alcohol towel to wipe the black residue off. Then i wipe down my jars with alcohol and insert needle, i redo this process for each jar. I also do all my work inside of a glove box.
I cant see how a resued syringe can be your problem if you are sterilising the needle prior to use. Unless of course u sucked in some air after originally using them but i doubt u would of done that. For them to be the reason of contamination then a contam needs to get inside the syringe, providing you are using very sterile procedures with everything else. Might just of been a case of bad luck.
I personally wouldnt worry bout re-injecting more spores if u are worried about possibly getin a contam, as long as the jars arnt dryin out the myc will soon enough end up getin around the jar just might take a couple weeks.
GL anyway.
-------------------- Old Ben I beleive that my job in mappin the impulse pathways of a single neuron can allow us to open a mecca into the quailitative functions of the human geno... Please support your local free spore ring by sendin back prints from successful grows, FSRE
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Kalix
'Head
Registered: 03/20/05
Posts: 1,504
Last seen: 18 years, 3 months
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Re: How do I make sure my syringe 100% sterile? [Re: BenKenobi]
#4422375 - 07/19/05 04:33 AM (18 years, 7 months ago) |
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I wouldn't put more spores in the B+, you are inviting bacterial contams that way, by throwing off the water content...
-------------------- My Unitarian Jihad Name is: The Shotgun of Sweet Reason
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A3eyedfish
mycophile
Registered: 05/06/99
Posts: 1,454
Loc: Wut some people Call Par...
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Re: How do I make sure my syringe 100% sterile? [Re: BenKenobi]
#4422380 - 07/19/05 04:37 AM (18 years, 7 months ago) |
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pour yourself about 4 ozs of h202, remove the needle from the partially used syringe, dip the syringe end into the peroxide, shake it off, seal the syringe end with a hot glue gun. When you are ready to to use it again, sterlize the needle & syringe, twist off the hot glue & replace the needle, you should be ready to go
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FooMan
Registered: 02/02/05
Posts: 8,957
Loc: Earth
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Re: How do I make sure my syringe 100% sterile? [Re: BenKenobi]
#4422410 - 07/19/05 05:07 AM (18 years, 7 months ago) |
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Quote:
BenKenobi said: i flame it for 5sec with a BIC lighter and give it a good rub with a soaked alcohol towel to wipe the black residue off. Then i wipe down my jars with alcohol and insert needle, i redo this process for each jar. I also do all my work inside of a glove box.
flame+alcohol+glove box=
Be careful! Lysol too.
-------------------- Quick WBS Prep
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BenKenobi
Enlightened 1
Registered: 05/22/05
Posts: 342
Loc: Europe
Last seen: 10 years, 10 months
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Re: How do I make sure my syringe 100% sterile? [Re: FooMan]
#4422421 - 07/19/05 05:21 AM (18 years, 7 months ago) |
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lol yer i flinch every time i spark it dont worry, hasnt happened yet thou so fingerscrossed.
-------------------- Old Ben I beleive that my job in mappin the impulse pathways of a single neuron can allow us to open a mecca into the quailitative functions of the human geno... Please support your local free spore ring by sendin back prints from successful grows, FSRE
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lemon_lw
Stranger
Registered: 10/17/04
Posts: 3,622
Loc: That Way
Last seen: 16 years, 6 months
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Re: How do I make sure my syringe 100% sterile? [Re: BenKenobi]
#4422517 - 07/19/05 07:22 AM (18 years, 7 months ago) |
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it lopks like you have tape on your jars. if you do take it off and there will be no need to re innoc unless you messed up the water content.
-------------------- In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz
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