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Shop: North Spore Injection Grain Bag   Amazon ½ Pint Jars, HEPA Filter, Perlite, Ultrasonic Humidifier

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OfflineNewbieS
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Well I'm about ready to begin...sterilization ?'s
    #4421716 - 07/19/05 02:16 AM (15 years, 9 months ago)

My main concern and step for error is the sterilization of everything before and after inoculation. I plan on washing out all of the jars with antibacterial dawn or something, then perform the steaming/boiling procedure, then once it's time to cool the jars I'm going to wash out a Rubbermaid container with the dawn, then spray oust or Lysol sanitizer in it, then put the jars in it until the syringe is ready to be used...all while wearing gloves the whole process. Does this sound sterile enough?


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Re: Well I'm about ready to begin...sterilization ?'s [Re: Newbie]
    #4421763 - 07/19/05 02:33 AM (15 years, 9 months ago)

Should work, if ya wanna be safe just take 10 seconds and wipe it down with alcohol after you wash everything, but if i heard you right, it should work, but remember oust isn't always as effective on surfaces as Lysol, oust was designed as an air sanitizer, so when you sterilize you work surfaces, use Lysol,
Take care


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Re: Well I'm about ready to begin...sterilization ?'s [Re: Newbie]
    #4421774 - 07/19/05 02:35 AM (15 years, 9 months ago)

Be sure you and the room you're in are clean, clean clothes and everything. Wipe any counters and what not down and spray some anti-bacterial aerosol around the air too. No drafts, etc. The set up sounds pretty good to me.


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Re: Well I'm about ready to begin...sterilization ?'s [Re: Trunkspace]
    #4421779 - 07/19/05 02:37 AM (15 years, 9 months ago)

Sounds good...I even have the surgical scrub to wash my hands/arms with :laugh:.


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Re: Well I'm about ready to begin...sterilization ?'s [Re: Newbie]
    #4421790 - 07/19/05 02:39 AM (15 years, 9 months ago)

hey better to get it right the first time then spend time wondering what went wrong right! :smile:
Take care


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Re: Well I'm about ready to begin...sterilization ?'s [Re: Newbie]
    #4421791 - 07/19/05 02:40 AM (15 years, 9 months ago)

My room is a pretty hot, humid room with air that pretty much never moves (i'm rarely in it) so would my closet be a suitable growing environment once the cakes have formed...if they form? :-P


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Re: Well I'm about ready to begin...sterilization ?'s [Re: Newbie]
    #4421810 - 07/19/05 02:46 AM (15 years, 9 months ago)

Well you closet yea, but I'm assuming you mean your Rubbermaid tanks with perlite in your closet :smile: . But definitely, just make sure you have them in a place you can do your air exchanges!
Take care


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Re: Well I'm about ready to begin...sterilization ?'s [Re: BetaDelta]
    #4421825 - 07/19/05 02:50 AM (15 years, 9 months ago)

Well that was my concern...the air in the closet won't be moving much and I don't know how fresh it will be...should I just let a humidifier run in the room and crack the closet door? It's a fairly small room...not much bigger than a walk in closet.


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Re: Well I'm about ready to begin...sterilization ?'s [Re: Newbie]
    #4421838 - 07/19/05 02:55 AM (15 years, 9 months ago)

best thing, I'd go get a hepa, their not that expensive, but not necessary although recommended. doing an air exchange several times a day...Your moving relatively small amounts of air (whats in your Rubbermaid containers) when compared to the amount of air that fills your room. Obviously you'd want all air to be clean but we did that. as long as theirs a LITTLE circulation in the room once and a while, it's really not a prob.
Take care!


Edited by BetaDelta (07/19/05 02:55 AM)


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Shop: North Spore Injection Grain Bag   Amazon ½ Pint Jars, HEPA Filter, Perlite, Ultrasonic Humidifier

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