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 honeyroasted
member
Registered: 06/30/01
Posts: 125
Last seen: 23 years, 15 days
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 peptone trials 
#441038 - 10/29/01 06:21 PM (23 years, 6 months ago) |
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My friend the kangaroo recently cooked up six jars of millet with filter disks, and another six jars with filter disks and about a teaspoon of peptone (soy based) added. He is going to be inoculating all of the jars tomorrow with puerto rican spores. Updates coming!
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sylo
addict
Registered: 10/29/00
Posts: 219
Last seen: 23 years, 16 days
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Keep us posted. My guess is that peptone will improve growth but not remarkably so. My main concern about peptone is that it could promote contams.
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 mycofile
Pooh-Bah
Registered: 01/18/99
Posts: 2,336
Loc: Uranus
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I just love it when people give a small idea about what they are doing, then just leave it dangling on the server, never to be updated......
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"From a certain point of view"
-Jedi Master Obi Wan Kenobi
PM me with any cultivation questions.
I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.
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altarego
member
Registered: 10/25/01
Posts: 130
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Re: peptone trials [Re: mycofile]
#486185 - 12/10/01 03:51 PM (23 years, 5 months ago) |
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I agree. There is nothing more depressing than reading a post from a newbie about some method that they are "going to" try, and then no results (most likely because they failed at it.)
I often want to give advice about getting ahead of yourself in this biz, but then I remember how it was for me in the beginning, how much needed to be learned the hard way.
Alas . . .
AE
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honeyroasted
member
Registered: 06/30/01
Posts: 125
Last seen: 23 years, 15 days
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Re: peptone trials [Re: altarego]
#486273 - 12/10/01 05:28 PM (23 years, 5 months ago) |
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lots of mold
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altarego
member
Registered: 10/25/01
Posts: 130
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Sorry to hear that . . .
Did you figure out why?
AE
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honeyroasted
member
Registered: 06/30/01
Posts: 125
Last seen: 23 years, 15 days
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Re: peptone trials [Re: altarego]
#487190 - 12/11/01 09:08 AM (23 years, 5 months ago) |
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interestingly, the jars that had the peptone added appeared to have significantly less agressive growth. go figure. the results may be screwed up due to a contaminated syringe or something, but my kangaroo doesn't think it's likely, considering how he inoculated other jars with the same syringe which he has since managed to fruit.
it's worth trying over again, IMO, but i'm not in the biggest hurry...
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mycofile
Pooh-Bah
Registered: 01/18/99
Posts: 2,336
Loc: Uranus
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thanks, even if it's not a helpful update, it's still good to hear. Sorry it contamed...
--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi
PM me with any cultivation questions.
I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.
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B.I.O.
old hand
Registered: 05/26/99
Posts: 458
Last seen: 2 years, 2 months
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Re: peptone trials [Re: mycofile]
#488862 - 12/12/01 03:39 PM (23 years, 5 months ago) |
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peptone is not really needed for cubensis-substrates...pept is just a booster lets say...you should check your sterilisation prozess and worry less bout the substrate...most of grain works fine...rice, millet, rye. oats...etc...whole cereals wor better cause of the the shell...but also the risk of contam is higher....so a good PC or autoclave is really nec. 4 good results...good luck anyway...
BiO.
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hermes3
journeyman
Registered: 07/17/00
Posts: 56
Last seen: 14 years, 6 months
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 Re: peptone trials [Re: B.I.O.]
#504752 - 12/30/01 11:17 AM (23 years, 4 months ago) |
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For simplicity and ease of lab procedures once I have established a viable culture I shift to grain to grain transfers. To keep my strains vibrant and to avoid senscing I vary the additives to my grain jars i.e. peptone a while then yeast a while and also vary the water mixtures (stream water with different percentages of horse manure tea). So I guess I found the best use of peptone and other additives is to give the mushroom a varied diet. I agree that your contams should not be coming from the peptone but somewhere else in your environment.
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Agarical
journeyman
Registered: 01/14/01
Posts: 21
Last seen: 23 years, 3 months
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Re: peptone trials [Re: hermes3]
#513862 - 01/07/02 11:18 PM (23 years, 4 months ago) |
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Interesting, I wonder if adding diffrent suppliments like peptone, yeast, soy, would prevent a grain substate from getting media specific just like it does for agar culture. I believe Stamets only recommends G3 spawn (3 transfers from grain) when doing grain to grain. Is this because the rye grain would get media specific, or is there another reason...Anyone ever try adding varied suppliments at every transfer and see how many grain to grain transfers can be done before going back to agar???thanks!
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hermes3
journeyman
Registered: 07/17/00
Posts: 56
Last seen: 14 years, 6 months
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Re: peptone trials [Re: Agarical]
#514216 - 01/08/02 09:53 AM (23 years, 4 months ago) |
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I am well beyond three with continuing successful flushes. Of course I then go from grain to straw for my crop but I don't think that should make a difference. I also make sure to take spore prints every so often. Whether or not the mycelium become media specific I don't want to rely on a limited gene pool for too long.
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