Home | Community | Message Board

MRCA Tyroler Gluckspilze
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Seeds Store Buy Cannabis Seeds, Buy CBD   Amazon Agar, Malt Extract

Jump to first unread post. Pages: 1
Today is thetomorow youworried aboutyesturday

Registered: 06/11/05
Posts: 67
Last seen: 12 years, 7 months
Question on cloning
    #4403225 - 07/14/05 01:09 PM (12 years, 9 months ago)

When this batch of mushrooms I am growing now is done I am interested in trying to clone. I have never don this before, but I am trying to expand my knowledge of the hobby. So, My plan is to take a peice of the base of a mushroom, let is sit in 10% h2o2 solution and then place into a lc made from malt extract. Is this correct? If not, what part is incorrect? Also assuming I am doing this correct, how long will it take for the lc to be finished. I know when using spores I wait about a week to use, but I am not sure what the timeline would be like with this. Any help would be appreciated.

Post Extras: Print Post  Remind Me! Notify Moderator
born to grow
 User Gallery

Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 6 years, 11 months
Trusted Cultivator
Re: Question on cloning [Re: Amazoniandreams]
    #4403265 - 07/14/05 01:27 PM (12 years, 9 months ago)

First off take the tissue sample from the largest specimen that is in a cluster/group this is always the best choice. 10% may be a bit on the strong side, I use 3% and take a sample from the inner core of the stem chop it up in the h2o2 let it sit from 20-30min. then transfer to agar, I haven't done LC with tissue but as long as you have something in the LC container to agitate and break apart the myc you should do just fine. Your judgement as to a time frame will depend on conditions and how "thick" a LC you want. GL

Getting the most out of your casings!, A pinning strategy.
Oyster Shell "Flour" $2 for 1lb. a hell of a deal :wink:
Not what is overlay but rather what overlay is
Gas Exchange vs. FAE

"We all have priorities. I used a closet once setup a nice little lab trouble was all the shit that was in there ended up in the bedroom that pissed off the GF then I ended up dumping her as she was getting in the way of my sterile culture technique! Ya I got priorities too!!!"

Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Shop: Original Seeds Store Buy Cannabis Seeds, Buy CBD   Amazon Agar, Malt Extract

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* Agar question sill_o_cin 1,783 4 11/21/01 07:23 PM
by sill_o_cin
* lotsa questions frozenballs 961 11 04/25/03 02:34 AM
by jakeholman
* H2O2 Question... cdlove69 1,263 3 04/18/02 04:35 PM
by cdlove69
* HoneyTek and Tissue Cloning questions... UnclePimpy 728 2 02/10/02 09:31 PM
by Zen Peddler
* Re: Making Clones??? 3Mshroom 602 1 04/06/00 03:13 PM
by PhilosoPossum
* My Clone Has The Blues KingCobWeb 650 5 11/20/02 04:48 AM
by Skikid16
* Few casing questions... Sick_Of_It_All 523 3 08/22/02 02:47 PM
by Sick_Of_It_All
* Hydrogen Peroxide questions... djcontra 1,730 13 06/05/01 05:02 PM
by pl151

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Roadkill, Magash, Shroomism, george castanza, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, cronicr, PussyFart, Tmethyl, Pastywhyte, bodhisatta
239 topic views. 18 members, 44 guests and 20 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
The HUP - Testing Kits
Please support our sponsors.

Copyright 1997-2018 Mind Media. Some rights reserved.

Generated in 0.02 seconds spending 0.005 seconds on 19 queries.