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kREATION1
kяедτιסи1
Registered: 07/14/01
Posts: 157
Last seen: 17 years, 7 months
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sterilizing
#440302 - 10/29/01 05:13 AM (23 years, 2 months ago) |
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Hello, this is the first time i am going to try to grow some mushrooms,im using pf-tek ,i just made 1 jar and i want to sterilize it.
but what i dont understand is when i am going to sterilize the jars in a pressure cooker is do i need to screw the lid up tightly, or loose?, or do i need to put some aluminium foil over the lid?, or should i only tape the holes and leave the lid loose ? or tight ?
thank you.
-------------------- "an immigrant from heaven on earth with a work visa"
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Teiro
Earth Entitie
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Re: sterilizing [Re: kREATION1]
#440328 - 10/29/01 05:50 AM (23 years, 2 months ago) |
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I would put the lid tight and also add the tin foil. This is to make sure that no water/vapor will enter your cake, filling it with water and making the formula wrong. It happens a lot with me (to get water inside my jars), its a pain.
-------------------- Life does not have to be necessarily long. It just has to be good. Be wise. Peace, Love, Flower Power.
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Snobrdr311
outdoorenthusiast
Registered: 09/03/01
Posts: 1,468
Loc: Midwest
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Re: sterilizing [Re: Teiro]
#440389 - 10/29/01 07:45 AM (23 years, 2 months ago) |
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yeah I think the same thing happened with me, I left them loose while boiling, the ratio definatly changed I believe, let's hope not though.
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kREATION1
kяедτιסи1
Registered: 07/14/01
Posts: 157
Last seen: 17 years, 7 months
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Re: sterilizing [Re: kREATION1]
#440491 - 10/29/01 09:33 AM (23 years, 2 months ago) |
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thank you, that's what i needed to know.
-------------------- "an immigrant from heaven on earth with a work visa"
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duncan8r
Scum
Registered: 08/07/01
Posts: 91
Loc: Syd, Australia
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Re: sterilizing [Re: kREATION1]
#440768 - 10/29/01 02:01 PM (23 years, 2 months ago) |
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Dear Guys,
I dont wanna be a stick in the mud, but you should leave the lids loose to allow steam to get in. The basis behind pressure cookers is summerised below (ripped shamelessly off the net)
Steam sterilization is a time proven and economical process of killing microorganisms through the application of ***moist heat (saturated steam) under pressure****. Heat damages the cell?s essential structures including the cytoplasmic membrane rendering the cell no longer viable.
The rate by which bacterial cells are thermally inactivated depends on the temperature and the time of heat exposure to which they are exposed.
Method:
In the presence of moisture, microorganisms are destroyed at a lower temperature than in dry heat. This is the method of choice when product can withstand such treatment.
10 lb Pressure (115.5 deg. C)...30 minutes
15 lb Pressure (121.5 deg. C)...20 minutes
20 lb Pressure (126.5 deg. C)... 15 minutes
You really need the moist steam to penentrate the jars to achieve sterilization.If you leave the lids on theres a chance of contamination in the middle of the jar surviving. Also the jars might crack
anyway, what would a simple simian know
D
-------------------- Not all chemicals are bad. Without chemicals such as hydrogen and oxygen, for example, there would be no way to make water, a vital ingredient in beer. --Dave Barry
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puscle
genius of love
Registered: 01/06/01
Posts: 4,539
Loc: NY
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Re: sterilizing [Re: duncan8r]
#440789 - 10/29/01 02:27 PM (23 years, 2 months ago) |
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Bullshit! Steam does not sneak in a loose lid. The presense of moisture exists in the substrate. Heat kills, not steam.
Edited by puscle (10/29/01 02:29 PM)
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Scourge
The Dr.
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Re: sterilizing [Re: duncan8r]
#440834 - 10/29/01 03:13 PM (23 years, 2 months ago) |
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heat kills. presure kills. the only reason to make steam is to raise the presure. as the presure get higher so does the boiling point allowing you get get your jars over the boiling point of an open pot.. more presure more heat, more heat more presure.
-------------------- "Those who walk in Love and Truth shall grow in Honor and Strength."
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ByaKKo
Stranger
Registered: 10/24/01
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Re: sterilizing [Re: kREATION1]
#440860 - 10/29/01 03:43 PM (23 years, 2 months ago) |
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aren't you guys taking into account there are the innoculation holes at the top?
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shitdog
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Registered: 10/24/01
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Re: sterilizing [Re: kREATION1]
#440912 - 10/29/01 04:33 PM (23 years, 2 months ago) |
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Kreation, Go to Psilocybe Fanaticus website he is the founder of the PF tek and he specificlly says: Leave the lids loose during sterilization SO the steam can penetrate the jars.
There are three thing you should worry about during sterilazation:
1 dont let the glass touch the pot- its too hot and it'll crack the glass.
2 don't let water slosh up into the jars- you know why.
3 don't let water fall off of the bottom of the lid onto the jars when your done, it might get in.
Just use your head and leave the lid loose.
-------------------- I'll never get out of this maze
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Jammer
Computers areMORE Addictive!
Registered: 11/04/00
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Re: sterilizing [Re: shitdog]
#441070 - 10/29/01 06:49 PM (23 years, 2 months ago) |
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Well I hesitate to say this... For starters I have a lot of respect for the founder of the modern day spore syringe tek. (PF TEK) However dont belive everthing that the tek says. My biggest problem is where he says to steam the jars in a PC for 20 minutes at 15psi. That was tried... A full 20 minutes of the steam valve whistleing away... all jars turned green within 3 days... Same thing time after time. Now, for my friends, many had 100% luck the first attempt at the PF Tek useing a steamer method (no PC) in basicly a large steel pot/lid steamed for 60 minutes. I mean it was the first atempt useing the TEK and 100% of the jars made it with NO PC USED! When they tried the PF TEK Pressure Cooker method (20 mins @ 15 psi) nothing but green jars. ... Not untill they raised the steaming time to about 45-60 minutes did they take. Also the PF Tek advises that cooking over 20 minutes at 15psi can dry the substrait.. Anyone here ever seen that happen? I havent. Also it has been shown that 60 minutes @ 10 PSI works peferctly fine! (Fixed pressure PC's of 10psi are extremely inexpensive)
ShroomWizard claims that placeing the lids on with the rubber side down and on screwed down tight will work... ' not sure about that one.
Either way, this litle debate might go on for awhile.
-------------------- >>Jammer>>
Edited by Jammer (10/29/01 07:17 PM)
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shitdog
member
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Re: sterilizing [Re: Jammer]
#441079 - 10/29/01 06:58 PM (23 years, 2 months ago) |
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Your so right! Thats exactly what happened to me. My first time, I used a big pot and Sterilized for 1.5 hours- no contams. This time I'm having big problems with this stupid pressure cooker.
Read my Post in Contam forum " my cakes have to go to the ER"
-------------------- I'll never get out of this maze
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Jammer
Computers areMORE Addictive!
Registered: 11/04/00
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Re: sterilizing [Re: shitdog]
#441087 - 10/29/01 07:04 PM (23 years, 2 months ago) |
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hmm... I think that were on to something here. It explains a lot..... a lot of stress!
(*grin*)
-------------------- >>Jammer>>
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shitdog
member
Registered: 10/24/01
Posts: 132
Loc: In a place far away from ...
Last seen: 23 years, 15 days
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Re: sterilizing [Re: Jammer]
#441096 - 10/29/01 07:09 PM (23 years, 2 months ago) |
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From now on I'm sterilizing for an hour and a half. I guess the only thing that would explain it is that it is the heat, not the steam. I'd like to experiment with this. The way to find out would be to sterilize acouple jars for anhour and a half with the lids tight and a couple with the lids loose, well you know the rest.
-------------------- I'll never get out of this maze
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