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BlimeyGrimey
Collector of Spores




Registered: 08/24/05
Posts: 3,787
Loc: Puget Sound
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Re: Psilocybe sierrae aka P. subfimetaria [Re: Workman] 2
#28615951 - 01/10/24 04:01 AM (17 days, 15 hours ago) |
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Closest I could find on a BLAST search is Ps. stuntzii. I couldn't find any Ps. sierrae ITS sequences available.
-------------------- Message me for free microscopy services on Psilocybe, Panaeolus, and Gymnopilus species. Looking for wild Panaeolus cinctulus and Panaeolus olivaceus prints.
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BlimeyGrimey
Collector of Spores




Registered: 08/24/05
Posts: 3,787
Loc: Puget Sound
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Re: Psilocybe sierrae aka P. subfimetaria [Re: the_chosen_one] 2
#28616610 - 01/10/24 04:41 PM (17 days, 2 hours ago) |
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Quote:
inski said: Yes, and it's macroscopically similar to P. stuntzii also.
I'm not very good at interpreting ITS data, but is there a chance this is Ps. stuntzii? Most of the Ps. stuntzii in the blast don't even match 100% to each other, and this ITS sequence even has some 99.5% matches with Ps. stuntzii. Once again though, I'm not very versed in ITS data.
Quote:
the_chosen_one said: This is awesome!
Hey Grimes! Good to see you around again! 
Hey TCO! I'm back! 
Over the next few months I'll be getting back into the hobby. Just got a scope after being scope-less for a decade.
-------------------- Message me for free microscopy services on Psilocybe, Panaeolus, and Gymnopilus species. Looking for wild Panaeolus cinctulus and Panaeolus olivaceus prints.
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BlimeyGrimey
Collector of Spores




Registered: 08/24/05
Posts: 3,787
Loc: Puget Sound
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Re: Psilocybe sierrae aka P. subfimetaria [Re: Workman]
#28621065 - 01/14/24 01:47 PM (13 days, 5 hours ago) |
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Well that settles a few things for me.
1. I can, somewhat, interpret ITS using Genbank BLAST.
2. Ps. stuntzii has many varying morphologies that seem to be more substrate-based than temp-based. I'll have to revisit CaptainFuture's grows to see if he used any manure substrates in his Ps. stuntzii grows.
Considering I couldn't find a single Ps. sierrae sequence online, is it a real species?
-------------------- Message me for free microscopy services on Psilocybe, Panaeolus, and Gymnopilus species. Looking for wild Panaeolus cinctulus and Panaeolus olivaceus prints.
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BlimeyGrimey
Collector of Spores




Registered: 08/24/05
Posts: 3,787
Loc: Puget Sound
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Re: Psilocybe sierrae aka P. subfimetaria [Re: Pscientist]
#28637055 - 01/27/24 03:14 PM (4 hours, 7 minutes ago) |
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Thank you for that rundown!
I'm not 100% convinced that even breeding studies would definitively identify multiple species as being synonymous. There seems to be evidence that species such as P. azurescens and cyanescens will breed and I believe most species within the same sect will probably breed, even if they don't do it often or readily.
Now I remember why I tend to stick to the hunting and cultivation of mushrooms instead of the taxonomy.
-------------------- Message me for free microscopy services on Psilocybe, Panaeolus, and Gymnopilus species. Looking for wild Panaeolus cinctulus and Panaeolus olivaceus prints.
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BlimeyGrimey
Collector of Spores




Registered: 08/24/05
Posts: 3,787
Loc: Puget Sound
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Re: Psilocybe sierrae aka P. subfimetaria [Re: Pscientist] 2
#28637181 - 01/27/24 05:06 PM (2 hours, 15 minutes ago) |
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Quote:
Pscientist said: These species can breed, and generate fruits that have spores that are themselves viable? That is quite fascinating.
The paper I am referring to is : Commentary on Psilocybe cyanescens Alexander Giessler
-------------------- Message me for free microscopy services on Psilocybe, Panaeolus, and Gymnopilus species. Looking for wild Panaeolus cinctulus and Panaeolus olivaceus prints.
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