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InvisibleBlimeyGrimey
Collector of Spores
Male


Folding@home Statistics
Registered: 08/24/05
Posts: 3,787
Loc: Puget Sound
Re: Psilocybe sierrae aka P. subfimetaria [Re: Workman] * 2
    #28615951 - 01/10/24 04:01 AM (17 days, 17 hours ago)

Closest I could find on a BLAST search is Ps. stuntzii. I couldn't find any Ps. sierrae ITS sequences available.



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InvisibleBlimeyGrimey
Collector of Spores
Male


Folding@home Statistics
Registered: 08/24/05
Posts: 3,787
Loc: Puget Sound
Re: Psilocybe sierrae aka P. subfimetaria [Re: the_chosen_one] * 2
    #28616610 - 01/10/24 04:41 PM (17 days, 4 hours ago)

Quote:

inski said:
Yes, and it's macroscopically similar to P. stuntzii also.




I'm not very good at interpreting ITS data, but is there a chance this is Ps. stuntzii? Most of the Ps. stuntzii in the blast don't even match 100% to each other, and this ITS sequence even has some 99.5% matches with Ps. stuntzii. Once again though,  I'm not very versed in ITS data.


Quote:

the_chosen_one said:
This is awesome!

Hey Grimes! Good to see you around again! :wave:




Hey TCO! I'm back! :cool:

Over the next few months I'll be getting back into the hobby. Just got a scope after being scope-less for a decade.


--------------------
Message me for free microscopy services on Psilocybe, Panaeolus, and Gymnopilus species.

Looking for wild Panaeolus cinctulus and Panaeolus olivaceus prints.


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InvisibleBlimeyGrimey
Collector of Spores
Male


Folding@home Statistics
Registered: 08/24/05
Posts: 3,787
Loc: Puget Sound
Re: Psilocybe sierrae aka P. subfimetaria [Re: Workman]
    #28621065 - 01/14/24 01:47 PM (13 days, 7 hours ago)

Well that settles a few things for me.

1. I can, somewhat, interpret ITS using Genbank BLAST.

2. Ps. stuntzii has many varying morphologies that seem to be more substrate-based than temp-based. I'll have to revisit CaptainFuture's grows to see if he used any manure substrates in his Ps. stuntzii grows.

Considering I couldn't find a single Ps. sierrae sequence online, is it a real species?


--------------------
Message me for free microscopy services on Psilocybe, Panaeolus, and Gymnopilus species.

Looking for wild Panaeolus cinctulus and Panaeolus olivaceus prints.


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InvisibleBlimeyGrimey
Collector of Spores
Male


Folding@home Statistics
Registered: 08/24/05
Posts: 3,787
Loc: Puget Sound
Re: Psilocybe sierrae aka P. subfimetaria [Re: Pscientist]
    #28637055 - 01/27/24 03:14 PM (6 hours, 10 minutes ago)

Thank you for that rundown!

I'm not 100% convinced that even breeding studies would definitively identify multiple species as being synonymous. There seems to be evidence that species such as P. azurescens and cyanescens will breed and I believe most species within the same sect will probably breed, even if they don't do it often or readily.

Now I remember why I tend to stick to the hunting and cultivation of mushrooms instead of the taxonomy.


--------------------
Message me for free microscopy services on Psilocybe, Panaeolus, and Gymnopilus species.

Looking for wild Panaeolus cinctulus and Panaeolus olivaceus prints.


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InvisibleBlimeyGrimey
Collector of Spores
Male


Folding@home Statistics
Registered: 08/24/05
Posts: 3,787
Loc: Puget Sound
Re: Psilocybe sierrae aka P. subfimetaria [Re: Pscientist] * 2
    #28637181 - 01/27/24 05:06 PM (4 hours, 18 minutes ago)

Quote:

Pscientist said:
These species can breed, and generate fruits that have spores that are themselves viable? That is quite fascinating.




The paper I am referring to is : Commentary on Psilocybe cyanescens Alexander Giessler


--------------------
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