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Offlinesludge
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Registered: 02/23/05
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g2g ??????
    #4397750 - 07/12/05 11:29 PM (11 years, 7 months ago)

how many times can you do a grain to grain transver before the mycel gives up?i have heard you can only go 3 generations before you have to start over from spores is this true???im on my secound generation right now and the mycel is going strong!!!!


--------------------
Down in a hole and I don't know if I can be saved
See my heart I decorate it like a grave
You don't understand who they
Thought I was supposed to be
Look at me now a man
Who won't let himself be


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InvisibleHolydiver
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Re: g2g ?????? [Re: sludge]
    #4397754 - 07/12/05 11:31 PM (11 years, 7 months ago)

3 generations is the general rule of thumb. The mycelium doesn't give up so to speak, but the genetics seem to go downhill fast beyond that point.


--------------------
To find a place to live between the negatives and positives.


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Offlinesludge
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Registered: 02/23/05
Posts: 707
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Re: g2g ?????? [Re: Holydiver]
    #4397770 - 07/12/05 11:36 PM (11 years, 7 months ago)

Quote:

Diver said:
3 generations is the general rule of thumb. The mycelium doesn't give up so to speak, but the genetics seem to go downhill fast beyond that point.


meaning the mushrooms dont develop good or what?and does this go the same for LC?


--------------------
Down in a hole and I don't know if I can be saved
See my heart I decorate it like a grave
You don't understand who they
Thought I was supposed to be
Look at me now a man
Who won't let himself be


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Offlinescatmanrav
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Re: g2g ?????? [Re: Holydiver]
    #4397773 - 07/12/05 11:37 PM (11 years, 7 months ago)

Even at 3 itll be strong..but 4 itll get slow and fruits will be poorer..after 5th you get very slow jars, some dont finish and weird mutations. It can turn out ok, but usually just gets crappy and isnt worth it..

Take it from someone who tried taking G2G transfers to far...you end up with a closet full of crap genetics that dont fruit for shit and lost strains, and set backs...the rule can be bent, but its a rule you shouldnt break, and try to follow..


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


Edited by scatmanrav (07/12/05 11:40 PM)


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Offlinesludge
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Re: g2g ?????? [Re: scatmanrav]
    #4399469 - 07/13/05 01:37 PM (11 years, 7 months ago)

when you inculate the jars with spores is that considered the first generation?


--------------------
Down in a hole and I don't know if I can be saved
See my heart I decorate it like a grave
You don't understand who they
Thought I was supposed to be
Look at me now a man
Who won't let himself be


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Offlinescatmanrav
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Re: g2g ?????? [Re: sludge]
    #4399476 - 07/13/05 01:40 PM (11 years, 7 months ago)

Yeah. If I shoot spores into an LC, then use the LC to shoot up a jar, I also call that first generation, though that may be a little more like 2nd.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleHolydiver
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Re: g2g ?????? [Re: scatmanrav]
    #4399490 - 07/13/05 01:43 PM (11 years, 7 months ago)

Quote:

scatmanrav said:
Yeah. If I shoot spores into an LC, then use the LC to shoot up a jar, I also call that first generation, though that may be a little more like 2nd.




That's still working on the 1st germination, and not an expansion of next mycelium set so much, so I'd consider spores->LC->jars to be first.


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Offlinesludge
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Re: g2g ?????? [Re: Holydiver]
    #4399558 - 07/13/05 02:01 PM (11 years, 7 months ago)

now what about just spores to grains no LC that would be 1st generation correct?


--------------------
Down in a hole and I don't know if I can be saved
See my heart I decorate it like a grave
You don't understand who they
Thought I was supposed to be
Look at me now a man
Who won't let himself be


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Offlinesludge
blunt rapter

Registered: 02/23/05
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Re: g2g ?????? [Re: sludge]
    #4399573 - 07/13/05 02:05 PM (11 years, 7 months ago)

i also have a ? on some jars i have colinised i have a couple that have wet spot the size of a dime can i still use them just not that spot throw it away in a sense?


--------------------
Down in a hole and I don't know if I can be saved
See my heart I decorate it like a grave
You don't understand who they
Thought I was supposed to be
Look at me now a man
Who won't let himself be


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Offlinescatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
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Re: g2g ?????? [Re: sludge]
    #4399658 - 07/13/05 02:22 PM (11 years, 7 months ago)

Quote:

Diver said:
Quote:

scatmanrav said:
Yeah. If I shoot spores into an LC, then use the LC to shoot up a jar, I also call that first generation, though that may be a little more like 2nd.




That's still working on the 1st germination, and not an expansion of next mycelium set so much, so I'd consider spores->LC->jars to be first.




Also my thought process on it, but I dont know "technically" what "stamets" would say...I still consider it first though and have taken from spores to LC to grain, then 2 transfers after that.

>now what about just spores to grains no LC that would be 1st generation correct?

Yes

Wet spot I have found to not be a huge issue when used as spawn or to case if you leave out the spot and everything else looks healthy...I'd say yes you can still use the jars, but understanding there are some risks as to how well it will perform. Fully healthy spawn always does better then unhealthy spawn..


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibleagar
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Registered: 11/21/04
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Re: g2g ?????? [Re: Holydiver]
    #4399687 - 07/13/05 02:28 PM (11 years, 7 months ago)

I go out three times (3) from whatever I start with.

Spore solution, agar isolate wedge from spores, or spores to LC is G1.

I consider an agar isolate tissue clone from any G1, to be G2.

When doing G2G - if you wish -- hold back a single colonized G1 jar in the fridge. Then go out to G3 - with the rest. Then - when those are at G3, revert back to the G1 jar in the fridge & go again.

Best bet I have found is G1 spores to LC, then refriderate the LC.

I have had some LC's last 6 months.
One below was started from spores 1/11/05 & pic taken today.


--------------------


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Offlinesludge
blunt rapter

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Posts: 707
Loc: somewhere in usa
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Re: g2g ?????? [Re: agar]
    #4399903 - 07/13/05 03:35 PM (11 years, 7 months ago)

cool thats what i needed to know!!thanks guys :rastamon:


--------------------
Down in a hole and I don't know if I can be saved
See my heart I decorate it like a grave
You don't understand who they
Thought I was supposed to be
Look at me now a man
Who won't let himself be


Post Extras: Print Post  Remind Me! Notify Moderator
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Amazon Shop for: ½ Pint Jars, Agar, Paul Stamets

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