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Amazon Shop: ½ Pint Jars, Wild Bird Seed

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Registered: 07/03/05
Posts: 427
How important is it to squirt along jar?
    #4392768 - 07/11/05 04:27 PM (12 years, 11 months ago)

If a single (center) hole of a 1/2 pint WBS flour cake is injected with 1cc of spores directly into center of cake, would it conlonize very slowly with a higher chance of contamination?

What is the purpose of trying to spread the spores on the interior of the jar glass?

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Brainy Smurf

Registered: 05/08/04
Posts: 11,483
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Re: How important is it to squirt along jar? [Re: FreedomFight]
    #4392790 - 07/11/05 04:34 PM (12 years, 11 months ago)

Of course it would colonize very slowly. When you inoculate the point is to get as much spore water to touch as many points on grains as possible (or points in your cake). All spore water contains spores so the better it spreads the more points will germinate, the faster everything colonizes. Water runs more down the side of the glass, then the middle of the substrate which is why you should aim towards the glass. 4 holes are used so there isnt one small stream of spore water down the middle, but 4 streams down oppisite sides that spread beter because the glass makes it slide more. Plus when you inject at the glass you can see signs of colonization immediatly..if you inject in the center you wont see anything until it hits the bottom or sides, days or weeks later.

"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay

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Registered: 08/07/04
Posts: 2,368
Re: How important is it to squirt along jar? [Re: scatmanrav]
    #4393096 - 07/11/05 06:03 PM (12 years, 11 months ago)

I inoculated one jar once in the middle in one hole with about 2.5 ccs. I saw nothing for about 6 days, while the others showed growth within 2-3. However, after that I could see tiny fluffs of white growing out in some spots, almost reaching the glass. This jar ended up being colonized just as fast as the others, which each got about 2ccs per jar. It was kind of neat watching it grow outwards.

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Amazon Shop: ½ Pint Jars, Wild Bird Seed

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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