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InvisibleZiggen
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How dumb is my friend?
    #4391320 - 07/11/05 02:59 AM (11 years, 5 months ago)

My friend thought that he had read everything there was to read, and thought that he had done everything right.
He inoculated a dozen pre-sterilized jars (bought from a sponsor who will not be mentioned because they're blameless) on Thurs. Tonight he had to toss 9 of em cause of trich and or cobweb.
Before innoc. he cleaned the hell out of his kitchen. Sprayed Lysol on all surfaces. took a shower. put on freshly washed clothes. Went into the kitchen, opened the jars (the bags holding 6 jars each, not the jars themselves), swabbed with alcohol, and put into alcohol cleaned future terrarium on its side. took syringes out of fridge, opened the bags they where in, swabbed the outside of them, put the syringes in the tub. Flame sterilized needle pulled in air, shook, and then pushed out excess air. Alcohol swabbed needle, and proceeds to innoc. 6 jars w/ it. Same for second syringe. He's thinking he fucked up cause he didn't re-sterilize syringe between each jar. All contams originated at innoc. points.
So 9/12 contaminated in pre-sterilized PF jars.
How dumb, exactly, is my friend? Say on a scale of 1-10

Sorry about the long post, by the way.

-Ziggen


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Invisiblemoonrockmushy
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Re: How dumb is my friend? [Re: Ziggen]
    #4391333 - 07/11/05 03:04 AM (11 years, 5 months ago)

sounds like an honest mistake. 2.3174/10


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OfflineIGnosticAbhorI
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Re: How dumb is my friend? [Re: moonrockmushy]
    #4391344 - 07/11/05 03:09 AM (11 years, 5 months ago)

I do it like this...

-Light torch.
-Heat needle.
-Swab cotton ball w/ alcohol.
-Swab needle..hold to cool.
-Swab innoc. point.
-Then quickly inject.

All done in open air. And it works fine.

You're friend went through all that trouble...DAMN.

I'd say it was the syringe...Where'd you get it from?

I'd say 1/10 :wink:

Gl next time

How are the other 3 doing?

-Gnostic


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InvisibleZiggen
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Re: How dumb is my friend? [Re: IGnosticAbhorI]
    #4391351 - 07/11/05 03:19 AM (11 years, 5 months ago)

He got 'em from Spores101. I doubt it was that though. 9 contamed, 3 doing great, but 1 syringe to 6 jars (I know more than needed, but he's spastic, and has a hard time w/ portion control). I have a feeling that they're the first one or two from each syringe.


Edited by Ziggen (07/11/05 03:20 AM)


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OfflineKalix
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Re: How dumb is my friend? [Re: Ziggen]
    #4391473 - 07/11/05 05:47 AM (11 years, 5 months ago)

The syringe was most likely fine until he sucked air into it!


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My Unitarian Jihad Name is: The Shotgun of Sweet Reason


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OfflineBetaDelta
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Re: How dumb is my friend? [Re: Kalix]
    #4391479 - 07/11/05 05:53 AM (11 years, 5 months ago)

Yea people who have had surgery and have had to inject them self with blood thinners will attest to this. The needles have a tiny air bubble in them for several reasons and that's one of them. You do not, not, remove the air bubble. It wont hurt it and it stays in perfect condition.


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Offlinebeyondsisxth
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Re: How dumb is my friend? [Re: BetaDelta]
    #4391554 - 07/11/05 07:16 AM (11 years, 5 months ago)

I agree with kalix, you shouldn't have sucked anything into the syringe.

Also, I found simply keeping the needle swabbed in an alcohol soaked sponge between jar injections and flamings cut down on my contam rate. When I first started this hobby I never kept the needle covered after flaming it and my contam rates were pretty bad. The last batch of jars I did where I used the sponge trick were contam free.


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The sun was pulling cheap shots doing commercial body tricks, Behind the back, Under the leg, I think he even did a headspin, On a crossfader that sounded whack, But looked excellent, All of the sudden it gets dim, The crater face steps in, Puts mexican drumbreaks on the Technics, He's like "Let's begin", He conducted an orchestra so dope the sun started sweatin' him, I guess he'd expected to win on pure artistic merit, Composing complex plays with nothin but soundbytes, Burned out the lights, Made MCs too self conscious quit the master mics, For a thousand nights, It continued without a single slip up, Except once the record skipped, But it kinda sounded cool.


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OfflineIGnosticAbhorI
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Re: How dumb is my friend? [Re: beyondsisxth]
    #4391600 - 07/11/05 08:05 AM (11 years, 5 months ago)

Quote:

sponge




God made cotton balls for a reason. :smirk:

Ask RK ,he's always got em =-O

-Gnostic


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OfflineOnetwothree
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Re: How dumb is my friend? [Re: IGnosticAbhorI]
    #4391735 - 07/11/05 10:06 AM (11 years, 5 months ago)

I dunno... When I do jars I flame then alcohol swab, and that's it. Only one time before I do all the jars. I did 18 jars in a row like that, no sterilization between jars. None of them contam'd... I really don't think that's what the problem was. Just me though... *shrug*

I agree that pulling in air and shaking probably wasn't a healthy thing to do.


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Free Spore Ring Canada


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Onlineultralight
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Re: How dumb is my friend? [Re: Kalix]
    #4391755 - 07/11/05 10:18 AM (11 years, 5 months ago)

Quote:

Kalix said:
The syringe was most likely fine until he sucked air into it!




Yup. I agree.


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Invisibleagar
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Re: How dumb is my friend? [Re: Ziggen]
    #4391760 - 07/11/05 10:22 AM (11 years, 5 months ago)

After you pull off the needle sheath, if you keep the needle wrapped at all times in a slightly alcohol saturated sterile gauze pad, it doesn't get exposed to open air.

You simply hold the pad between your fingers, press the needle into whatever you are inoculating & hold the pad against the surface. when you withdraw the needle, you simply pull it back up into the pad - then repeat the process.

OR.........flame the needle
You can buy a wickless alcohol lamp - under $20 on eBay.


Or, if you want to spend bigger bucks, buy one of these;


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Invisiblefinding_self
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Registered: 05/15/05
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h [Re: ultralight]
    #4392486 - 07/11/05 03:08 PM (11 years, 5 months ago)

h


Edited by finding_self (04/18/06 09:43 PM)


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Offlinecarl
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Re: How dumb is my friend? [Re: finding_self]
    #4392942 - 07/11/05 05:12 PM (11 years, 5 months ago)

ummm how sure is he that it is contams. Cuz thats what any newb will think when they see anything in their jars. Its pretty fucking hard to get contams when the cakes are still in jars. Shit i just leave my syringes laying around. Grab one stick it in the jars and let it rip. No alcohol or flame. THe only time i have ever gotten a contam was when i was starting casings for my first time. It was like the fourth flush and the casing was oversaturated.... i was basically asking for the mean green.


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InvisibleZiggen
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Re: How dumb is my friend? [Re: carl]
    #4393020 - 07/11/05 05:32 PM (11 years, 5 months ago)

blue-green and grayish stuff that almost 50% colonized one half pint jar in less than 24, about 10x faster than any snowy white mycelium that was also showing. He's pretty sure, and wishes that he had a digital camera. Would have definitely posted pics in contam forum before chucking if he had one. But he's pretty sure it was contams.


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Amazon Shop for: ½ Pint Jars, Scales

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