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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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OfflineJon
Registered: 06/28/03
Posts: 961
Last seen: 1 year, 11 months
PDA + H2o2 questions
    #4386921 - 07/09/05 03:34 PM (11 years, 5 months ago)

I transferred 3 pieces of mycelium from a colonized plate to 3 plates of PDA with a small amount of peroxide. I heard about the gestation period for the mycelium to adapt to the peroxide, tho its been about 8 days and the segment is the only thing showing signs of growth. The segments are showing fluffy growth but are very slowly making its way onto the rest of the agar. I thought condesation was a problem to I put the plates upside down in the incubator. Now they are significantlyy dry, but the mycelium on the segment still seems healthy. Should I worry about anything?


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OfflineJon
Registered: 06/28/03
Posts: 961
Last seen: 1 year, 11 months
Re: PDA + H2o2 questions [Re: Jon]
    #4388185 - 07/10/05 12:07 AM (11 years, 5 months ago)

bump


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Offlinered914
Minion
Registered: 11/05/04
Posts: 134
Loc: Utah
Last seen: 11 years, 2 months
Re: PDA + H2o2 questions [Re: Jon]
    #4388318 - 07/10/05 12:53 AM (11 years, 5 months ago)

Any smells from the plates? temp set right? Good batch of agar? Play with the tempeture a bit. Some times, I think the myc is watching me and experimenting by not growing and seeing what I will do. Take a hostage, show the other plates ya means business. Let em see what happens when they stall. I assume we are working with petri dishes here? You might need just a wiff more air in the plates. I use mining sample bottles, sometimes they stall if they don't get enough air.


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OfflineJon
Registered: 06/28/03
Posts: 961
Last seen: 1 year, 11 months
Re: PDA + H2o2 questions [Re: red914]
    #4388664 - 07/10/05 02:21 AM (11 years, 5 months ago)

No smell, I thought about the air problem and I removed some of the polyethyline sealer to see if that might make it more breathable. I guess in situations like this the best thing to do is just wait.


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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