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 HippieChick
Chicks can do it too!
 
Registered: 02/20/05
Posts: 5,958
Loc: Midwest
Last seen: 1 month, 30 days
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 Is a LC still viable. 
#4382729 - 07/08/05 04:32 AM (12 years, 11 months ago) |
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after being frozen ? 
Peace,Love,Happiness and Harmony.
 Hippie Chick 
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Peace,Love and Happiness
HC
Freedoms just another word for nothing left to lose..............
I LUV My Greenhouse
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 Magash
Da Bud Guru
Registered: 07/25/02
Posts: 5,875
Loc: Near Hilo
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Re: Is a LC still viable. [Re: HippieChick]
#4382734 - 07/08/05 04:37 AM (12 years, 11 months ago) |
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I would have to say no, but weirder things have happened. Try it on a small amount of substrate to see what happens.
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All creatures tremble when faced with violence. All creatures fear death, all love life. If we can only see ourselves in others, then how could we possibly hurt another creature?
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HippieChick
Chicks can do it too!
Registered: 02/20/05
Posts: 5,958
Loc: Midwest
Last seen: 1 month, 30 days
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Re: Is a LC still viable. [Re: Magash]
#4382750 - 07/08/05 04:42 AM (12 years, 11 months ago) |
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Thanks Mag. Had two in the back that froze some. I'll try them on a couple jars. Should be interesting.
Peace,Love,Happiness and Harmony.
Hippie Chick
--------------------
Peace,Love and Happiness
HC
Freedoms just another word for nothing left to lose..............
I LUV My Greenhouse
http://www.shroomery.org/forums/showflat.php/Number/5545848#5545848
My First Pans
http://www.shroomery.org/forums/showflat.php/Number/6212058#6212058
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blackout
Microwaver
Registered: 07/17/00
Posts: 4,901
Last seen: 2 months, 24 days
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Re: Is a LC still viable. [Re: HippieChick]
#4382863 - 07/08/05 06:10 AM (12 years, 11 months ago) |
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Please let us know how you get on. It is a good simple and cheap experiment. Most would say it will be killed due to cell rupture. Many said spores cant be frozen and Anno proved they were still viable. I have heard azure myc survives freezing.
If frozen cubensis LC's survive it would be brilliant, I don't like to store stuff in my fridge but the freezer is easier to hide stuff in from visitors.
Magash- Great ass!
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scatmanrav
Brainy Smurf
Registered: 05/08/04
Posts: 11,483
Loc: 
Last seen: 5 years, 5 months
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Re: Is a LC still viable. [Re: blackout]
#4383128 - 07/08/05 10:19 AM (12 years, 11 months ago) |
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I would bet if only partially frozen, most of it should still be viable and it would still work. If the whole thing froze solid, might be a whole lot less viable, who knows though..I havnt seen to much on frozen LCs.
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blackout
Microwaver
Registered: 07/17/00
Posts: 4,901
Last seen: 2 months, 24 days
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Re: Is a LC still viable. [Re: scatmanrav]
#4431925 - 07/21/05 04:18 AM (12 years, 11 months ago) |
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HippieChick - did you ever get around to testing the frozen syringe?
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HippieChick
Chicks can do it too!
Registered: 02/20/05
Posts: 5,958
Loc: Midwest
Last seen: 1 month, 30 days
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Re: Is a LC still viable. [Re: blackout]
#4431981 - 07/21/05 04:33 AM (12 years, 11 months ago) |
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I'll do it today. Have some other things I need to test.
Peace,Love,Happiness and Harmony.
Hippie Chick
--------------------
Peace,Love and Happiness
HC
Freedoms just another word for nothing left to lose..............
I LUV My Greenhouse
http://www.shroomery.org/forums/showflat.php/Number/5545848#5545848
My First Pans
http://www.shroomery.org/forums/showflat.php/Number/6212058#6212058
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Thumbprint
Stranger
Registered: 12/08/12
Posts: 4
Last seen: 5 years, 3 months
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I would've recomended thawing and keeping warm for a couple days, freezing while wet seems to kill significant amounts, but not all of the spores. frozen syringes don't colinate substrate as fast etc. If this happens just let it warm and rehab for a little bit.
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shro.om
Myco-holic
Registered: 11/10/12
Posts: 12
Loc: Maldives
Last seen: 5 years, 6 months
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Re: Is a LC still viable. [Re: Thumbprint]
#17384221 - 12/13/12 06:43 AM (5 years, 6 months ago) |
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looking forward to the results 
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k00laid
NEMO
Registered: 05/03/10
Posts: 19,539
Last seen: 13 hours, 40 minutes
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Re: Is a LC still viable. [Re: shro.om]
#17384229 - 12/13/12 06:49 AM (5 years, 6 months ago) |
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Quote:
shro.om said:
looking forward to the results
well we've been waiting 7 years so far.
maybe she'll come back.
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AMU - AMU Q & A - MyVideo Teks!
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