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 Lapius
Super Fighting Robot
Registered: 04/05/02
Posts: 308
Loc: Earth 25XX
Last seen: 9 years, 1 month
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 PDYA LC question 
#4373838 - 07/05/05 09:28 PM (17 years, 8 months ago) |
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Here is the recipe used:
-PDYA-
500ml water
5 grams of instant potato flakes(Potato Buds Brand)
9 grams agar agar
10 ml honey (Raw natural pure honey - Beemaid Brand)
1 gram of Nutritional Yeast
This was all combine in a quart jar and PC'd @ 15 PSI for 45 mins. Once the quart jar was cooled enough to touch, it was transported to my lab. Preparation for filtering Ala coffee filter in thru a funnel and finally into a baby bottle retro fitted per Agar's BB tek. (with one exception, a FD cut specific to the bottle)
I get everything filtering, filled 1 up 4oz. Went on to #2. Left everything to sit for a 1/2 hour to do some menial tasks. When I came back, everything was more gelatinous than words can describe. SUPER thick and most deff unusable.
So what did I do wrong? I thought many ppl made LC using agar agar and a nutritional base, or was I wrong? Any help would be appreciated.
And yes, the recipe was followed and measured completely in lab like atmosphere, lab tools and exceptional care. Kind of disheartening, but then I am all over it : "oh boy! I made a mistake, time to learn from it" Synapses Firing
>On further reflection, was this correct? Am I wrong in assuming that an Agar culture recipe for growing mycelium will not make a fine liquid culture? It is for petri and petri alone?
I had a glass bead agitator added to the Baby Bottle, while filtering I noticed even in a somewhat liquid consistency the bottom liquid was hardening extremely firm. So firm that the agitator was almost fixed an immovable. What went wrong?
Edited by Lapius (07/05/05 10:15 PM)
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 Pinback
Stranger
Registered: 07/20/02
Posts: 835
Loc: Europe
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 Re: PDYA LC question [Re: Lapius]
#4374515 - 07/06/05 01:14 AM (17 years, 8 months ago) |
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The purpose of the agar is to solidify the medium, so it is not fitting for a liquid culture. If you leave out the agar from the recipe, you will get a liquid medium with the same nutrients as the original recipe.
Note that if you filter the medium after sterilization, you either have to do it aseptically, or resterilize it afterwards.
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Anno
Experimenter
Registered: 06/17/99
Posts: 24,156
Loc: my room
Last seen: 1 hour, 22 minutes
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Re: PDYA LC question [Re: Lapius]
#4374530 - 07/06/05 01:17 AM (17 years, 8 months ago) |
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What pinback said.
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agar
old hand
Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: PDYA LC question [Re: Lapius]
#4374649 - 07/06/05 02:05 AM (17 years, 8 months ago) |
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The tater flakes & yeast will get you a milky solution, which IMHO is not a good thing.
I would suggest a 3% solution of karo -or- dextrose and/or light dry malt to water. Karo -or- dextrose is clear. Light dry malt will be a little gold colored. PC 15 or 20 minures.
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blackout
Registered: 07/16/00
Posts: 5,187
Last seen: 3 months, 3 days
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Re: PDYA LC question [Re: agar]
#4374685 - 07/06/05 02:30 AM (17 years, 8 months ago) |
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Yep, if it is cloudy it is hard to see what is happening inside, and harder to spot unusual growth (contams).
Don't toss what you made. It should make some good agar plates, thought usually you would add about 1.5g agar per 100ml.
Use agars recipie or use your honey to make a 2-4% solution
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Roadkill
Retired Shroomery Mod
Registered: 12/11/01
Posts: 22,674
Loc: Montana
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 Re: PDYA LC question [Re: Lapius]
#4375459 - 07/06/05 11:23 AM (17 years, 8 months ago) |
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Quote:
Lapius said:
Here is the recipe used:
-PDYA-
500ml water
5 grams of instant potato flakes(Potato Buds Brand)
9 grams agar agar
10 ml honey (Raw natural pure honey - Beemaid Brand)
1 gram of Nutritional Yeast
You used my agar recipy for a LC is the problem.
You don't use this recipy for making liquid cultures.
Use the karo tek or a malt based tek for LC's.
I don't know how you got mixed up on this bro.
I hope it wasn't something I said.
tc
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Laterz, Road
Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!
Brainiac said:
PM the names with  on there names, that means they have mushrooms for sale.
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