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OfflineBlackCat0315
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Is this normal growth for 6 days with WBS ?
    #4366874 - 07/03/05 04:53 PM (18 years, 8 months ago)

Hey fellow shroomers,
Is this normal growth for 6 days with WBS:



Closeup:


I thought I had read that WBS colonizes much faster than BRF.
These jars of BRF were knocked up the same day and show much more progress:








...am I doing something wrong ?

Thanks !

Edited by BlackCat0315 (07/03/05 07:42 PM)

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Offlineoysterguy63
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Re: Is this normal growth for 6 days with WBS ? [Re: BlackCat0315]
    #4366888 - 07/03/05 05:02 PM (18 years, 8 months ago)

Normal.


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Offlinexburn
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Re: Is this normal growth for 6 days with WBS ? [Re: BlackCat0315]
    #4366889 - 07/03/05 05:03 PM (18 years, 8 months ago)

how did you knowck the up. in like 2 days shake the shit out of them and they will will colonize real fast like.


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OfflineBlackCat0315
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Re: Is this normal growth for 6 days with WBS ? [Re: xburn]
    #4366898 - 07/03/05 05:09 PM (18 years, 8 months ago)

The WBS jars were shook well right after inoculation, and then once more at 1st signs of mycelium.

This is my 1st try with WBS, so I'm kind of concerned.

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Offlinescarymidgets
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Re: Is this normal growth for 6 days with WBS ? [Re: BlackCat0315]
    #4366903 - 07/03/05 05:11 PM (18 years, 8 months ago)

shakein at 1st sign can make them shock a little takein longer
i believe the rule of thumb is shake once at 30 % colonization


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Offlineunknown22
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Re: Is this normal growth for 6 days with WBS ? [Re: scarymidgets]
    #4367012 - 07/03/05 06:06 PM (18 years, 8 months ago)

I also found that skipping the simmering step, and just soaking for 12-18 hrs. speeds it up a little quicker. I found that simmering release starchs that slows down colonization

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Invisiblebackupwards2
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Re: Is this normal growth for 6 days with WBS ? [Re: unknown22]
    #4367021 - 07/03/05 06:10 PM (18 years, 8 months ago)

simmering may not be necessary, but if you do, you just have to rinse them well several times and the starches are washed away.
scarymidgets gave you some good advice too.
peace


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OfflineBlackCat0315
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Re: Is this normal growth for 6 days with WBS ? [Re: backupwards2]
    #4367238 - 07/03/05 07:38 PM (18 years, 8 months ago)

Thanks for all of the good advice. I'll post how they turn out...At least I have the cakes even if the WBS venture is a flop.

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Invisibleagar
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Re: Is this normal growth for 6 days with WBS ? [Re: BlackCat0315]
    #4367357 - 07/03/05 08:21 PM (18 years, 8 months ago)



This is at six days - OPTIMAL conditions



Here is after a shake @ 20% colonized.

Usualy takes about 12 days, sometimes 14.

Only needs shaking ONCE - between 20 to 30% colonized.
Anymore shaking (imho) slows it all down.


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OfflineBlackCat0315
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Re: Is this normal growth for 6 days with WBS ? [Re: agar]
    #4367480 - 07/03/05 08:54 PM (18 years, 8 months ago)

Thanks Agar,
I thought my WBS was going slow...your photos confirmed it. Do you think there's any hope for these to colonize ?

I guess I may have messed them up by shaking them to soon.

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InvisibleLouiseLouise
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Re: Is this normal growth for 6 days with WBS ? [Re: BlackCat0315]
    #4367574 - 07/03/05 09:28 PM (18 years, 8 months ago)

Don't be so quick to blame yourself. Looks like you got every thing right.
Sometimes some spore solutions or w/e are faster than others. It's just a natural occurance.
Anyway, your brf jars are aggressive :eek: so, it is apparent you're doing everything right, just let them take they're course.

Good Luck  :peace:


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OfflineBlackCat0315
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Re: Is this normal growth for 6 days with WBS ? [Re: LouiseLouise]
    #4367615 - 07/03/05 09:39 PM (18 years, 8 months ago)

Thanks for the vote of confidence !

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Invisibleagar
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Re: Is this normal growth for 6 days with WBS ? [Re: BlackCat0315]
    #4367635 - 07/03/05 09:44 PM (18 years, 8 months ago)

Multispore inoculation can have weird timing.

Sometimes it takes a week - before you even see germination.

Once it goes - it grows. Sometimes fast, sometimes slow.

Differing spore age, strain, moisture, media & other variables ALL have a lot to do with it.

Yours should be fine, just don't shake them - more than needed.


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Invisiblegema
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Re: Is this normal growth for 6 days with WBS ? [Re: unknown22]
    #4367840 - 07/03/05 11:21 PM (18 years, 8 months ago)

Quote:

unknown22 said:
I also found that skipping the simmering step, and just soaking for 12-18 hrs. speeds it up a little quicker. I found that simmering release starchs that slows down colonization




i wonder if simmering also makes wbs susceptible to contams which might explain my high jar turnover.

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Offlinepcubmycol
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Re: Is this normal growth for 6 days with WBS ? [Re: gema]
    #4368003 - 07/04/05 12:10 AM (18 years, 8 months ago)

That growth looks very slow to me. My pint jars of WBS only take 5-6 days with LC... 2-3days til 30% then 2-3days til finish after the shake. Did you use LC or multi-spore blackcat? I thought I remembered you asking about LC not too long ago?


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OfflineBlackCat0315
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Re: Is this normal growth for 6 days with WBS ? [Re: pcubmycol]
    #4368718 - 07/04/05 05:37 AM (18 years, 8 months ago)

Hey Pcubmycol,
The WBS was injected with spore syringe into tyvek covered hole in lid.

I've not tried the LC I started yet.

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Offlinepcubmycol
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Re: Is this normal growth for 6 days with WBS ? [Re: BlackCat0315]
    #4368850 - 07/04/05 07:26 AM (18 years, 8 months ago)

ok, well, your growth is just normal then... just slow germination i guess...


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