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Offlineblueboy353
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can you clone from dried mushrooms
    #4360062 - 07/01/05 04:50 PM (11 years, 5 months ago)

i have dried mushrooms, can youclone them some how. or is their no luck b/c their dried.


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Offlineshirley knott
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Re: can you clone from dried mushrooms [Re: blueboy353]
    #4360176 - 07/01/05 05:16 PM (11 years, 5 months ago)

it is possible to reanimate the host if the mushrooms was dried with mature spores on the gills. you could try selecting a piece of gill and putting it on agar, then crossing your fingers and transferring to a clean plate if a white fluffy growth emerges.
if you're lucky, someone like workman, eontan or rogerrabbit will happen by with details.


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InvisibleDragonaut
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Re: can you clone from dried mushrooms [Re: shirley knott]
    #4360237 - 07/01/05 05:31 PM (11 years, 5 months ago)

that's not really a clone though. you have to use tissue for cloning.


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Re: can you clone from dried mushrooms [Re: Dragonaut]
    #4360272 - 07/01/05 05:41 PM (11 years, 5 months ago)

no but it's the best he can expect


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InvisiblePinback
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Re: can you clone from dried mushrooms [Re: Prisoner#1]
    #4360416 - 07/01/05 06:23 PM (11 years, 5 months ago)

Check out this thread, and please try it yourself as well.


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Offlineshirley knott
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Re: can you clone from dried mushrooms [Re: Pinback]
    #4360546 - 07/01/05 06:58 PM (11 years, 5 months ago)

that was a great read, thanks for 'reviving' it, Pinback  :wink:

seems like the evidence that spores are NOT responsible for the reanimation RR and others proclaim possible, is impossible to disprove. you'd need to electron-microscope the sample before growth attempts, but this process of EM-preparation would surely itself render the tissue dead. maybe it's possible within a limited timeframe, maybe it's possible indefinitely, maybe it's impossible. i noted with interest roger's assertion that peroxide exposure limits the tek's success.


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OfflineRogerRabbitM
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Re: can you clone from dried mushrooms [Re: shirley knott]
    #4363162 - 07/02/05 03:28 PM (11 years, 5 months ago)

Many of the edible culture banks store mycelium in its dry form. The commercial mushroom farms routinely buy dry mycelium to restart on agar. It helps if there are no imperfect mold spores on the tissue. Bacteria usually isn't a problem as it is killed by drying.

Perhaps a segment in my upcoming video series can be to take a piece of mycelium from the inside of a fresh mushroom stipe as though I were going to clone it, but instead slip it inside a petri dish with desiccant rather than agar. I could let the tissue dry for a few months, under sterile conditions, then retrieve and grow it out. This is how the tissue banks do it. Dry mycelial tissue will grow again, I assure you. I've switched over to all legal edibles and medicinals, so I'd have to do it with oyster or something, but you'd get the point. The procedure would be the same.
RR


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Offlineblackout
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Re: can you clone from dried mushrooms [Re: RogerRabbit]
    #4368584 - 07/04/05 04:55 AM (11 years, 4 months ago)

That would be good to see.
How long does tissue last in H2O2.
I picked a fruit in open air, tore it up with dirty hands and tossed it in a jar of H2O2 for about 10mins, I then sucked up some tissue in a syringe through a self healing hole and injected it in a jar of grain. 2 days later I have growth started in the jar. It was 3% H2O2. Do you think it will last? Am I better leaving it in sunlight to speed up the degradation into H2O2.


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OfflineRogerRabbitM
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Re: can you clone from dried mushrooms [Re: blackout]
    #4368975 - 07/04/05 11:01 AM (11 years, 4 months ago)

Peroxide will kill some of the bacteria, but there's way more contaminants than just bacteria, especially working with dirty hands in open air. Unfortunately, I give your experiment little chance of success. I hope it succeeds, but the odds are against you.
RR


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Offlineblueboy353
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Re: can you clone from dried mushrooms [Re: RogerRabbit]
    #4371531 - 07/05/05 09:51 AM (11 years, 4 months ago)

allready molded :frown:


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Offlinemattymonkey
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Re: can you clone from dried mushrooms [Re: blueboy353]
    #4372463 - 07/05/05 04:23 PM (11 years, 4 months ago)

great thread, nice read pinback thanks...

so is this a way to store strains long term?

would it be better to use dried myc from an agar plate to get a strain thats a little closer to its origins?

im not really understanding the exact process... if placing a piece of mushroom tissue, or a agar wedge, in an empty plate.. would you seal the plate with parafilm still? would it dry out otherwise? how would one add desiccant to the plate, wouldnt the desiccant itself be contaminated? is it possible to sterilize it?
thanks


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OfflineRogerRabbitM
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Re: can you clone from dried mushrooms [Re: mattymonkey]
    #4377507 - 07/06/05 10:32 PM (11 years, 4 months ago)

The way I do it is in front of my flowhood, I cut a piece of mycelium from the very center of a thick stipe. This gets 'virgin' tissue. I place this small piece of tissue on a piece of wax paper and place in a clean petri dish with desiccant under the wax paper. I then seal it up with parafilm, desiccant and all. I've pulled two year old cracker dry tissue out this way and it began growing within two weeks after placing on agar. Mix the agar a bit weak, so it's wetter than normal. Make a transfer as soon as you can see mycelium growing.
RR


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OfflineOwl
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Re: can you clone from dried mushrooms [Re: RogerRabbit]
    #4382422 - 07/08/05 03:08 AM (11 years, 4 months ago)

Great thread, it inspired me to try this with a Pleurotus eryngii plate that dried up completely.

I prepared some MYA using a little bit less agar than usual to make the solidified medium a bit more moist. I inoculated 5 dishes with chunks of dry colonized agar.

Two days later I saw new growth in 3 dishes.

I am very happy with this tek because I thought I had lost this species permanently. It shows that these perfect fungi are amazingly resilient organisms.


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OfflineRogerRabbitM
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Re: can you clone from dried mushrooms [Re: Owl]
    #4386246 - 07/09/05 09:30 AM (11 years, 4 months ago)

Transfer some of that growth as soon as you see it. If it dried up on a plate, it was a slow-ride into stasis and contaminants are probably right behind the mycelium. Just a suggestion.
RR


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OfflineOwl
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Re: can you clone from dried mushrooms [Re: RogerRabbit]
    #4389486 - 07/10/05 12:28 PM (11 years, 4 months ago)

Good suggestion thanks.

I will make some transfers today...


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Offlineblackout
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Re: can you clone from dried mushrooms [Re: Owl]
    #4394958 - 07/12/05 05:26 AM (11 years, 4 months ago)

From TMC
"KOH Potassium Hydroxide, an agent commonly used to revive dried mushroom material for microscopic study at a concentration of 2.5%"

Anybody use this?


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OfflineWorkmanV
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Re: can you clone from dried mushrooms [Re: blackout]
    #4398112 - 07/13/05 01:14 AM (11 years, 4 months ago)

"Revive" in this context is not reanimate but more like rehydrate for viewing structures under the microscope. You can't slice or microscopically view dried material directly. It must be plumped up with water. KOH is caustic (think drain cleaner) and wouldn't be helpful in cultivation. The addition of KOH causes some color changes in certain microscopic features that can help in identification, but I have never been clear on what else it is for. It must make the structures clearer for viewing or aid in the reconstitution in some way since it is used for all dried mushroom tissue destined for the microscope. I purchased a supply of KOH but have yet to make a solution with it. NaOH is supposed to be a reasonable substitute.


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Offlineblackout
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Re: can you clone from dried mushrooms [Re: Workman]
    #4398721 - 07/13/05 04:55 AM (11 years, 4 months ago)

Makes sense now you say it. I am so used to seeing the term "microscopic study" in a disclaimer context that it didn't even register with me that it may mean that its not intended for growing.


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Offlinered914
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Re: can you clone from dried mushrooms [Re: blackout]
    #4403207 - 07/14/05 01:02 PM (11 years, 4 months ago)

I have heard of using "Ice Melter" from Home depot to dry stuff. It is some kind of salt, a little easier on your concrete if you actually deal with ice at all. Ya use it in crystal form, not solution to dry stuff. It is so dry where I live that trying to fruit a 10 lb. sawdust block of namekos will dry out compleatly in 24 hrs. Misting? Waist of time. Hose! Ya have to rethink your procedures around here.
Will most mushrooms rehydrate to working myc after drying? Ya mean all those agar dishes I throw out because they looked like potatoe chips could have been saved? I had to use short widemouth jars instead of petri dishes to control moisture loss even in a closed box. Anything you can add to rehydrating tissue would be very useful.


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Re: can you clone from dried mushrooms [Re: red914]
    #4405320 - 07/14/05 09:45 PM (11 years, 4 months ago)

If you live in a desert climate, you'll need to run a humidifier in the rooms you grow in. In fact, running a huge humidifier in your house would be a good idea. That will make it much easier to keep your grow areas humidified. It will also make it easier for you to breathe and keep your skin from drying out. Petri dishes should be wrapped with parafilm to hold in moisture and keep out contaminants.
RR


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