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Offlinenewmodel
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Syringes and inoculation
    #4350585 - 06/29/05 02:46 PM (12 years, 2 months ago)

Hey everyone,

  I was reading the PF tek and I a few questions popped into my head that I could not find an answer for...or maybe i missed it. When the syringes are recieved what are the optimal conditions that I should store the syringes at? Also, when i begin inoculation should i stick the needle all the way in tell the base of the syringe touches with the lid of the jar?  :smirk:

Like alwyas thanks to all. :mushroom2:


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Freedom is something that dies unless it's used


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Offlinepsilocyben
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Re: Syringes and inoculation [Re: newmodel]
    #4350616 - 06/29/05 02:56 PM (12 years, 2 months ago)

as long as they are going to be used within a week or so, they'll be fine at room temp.
for "long term"storage, you will want  to refrigerate it.

when you're innoculating, you may press the syringe all the way down.

here is one common mistake that i have found when innoculating, though:
i always begin wiith a flame sterilization. (this kills anything inside the needle that a wipe-down would not)
then wipe it down w/ an alcohol-soaked whatever.
make sure that you wipe it w/ alcohol between EACH inoculation point.
:zoom:


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Offlinenewmodel
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Re: Syringes and inoculation [Re: psilocyben]
    #4350678 - 06/29/05 03:12 PM (12 years, 2 months ago)

You mean all they way into the substrate twll the base of the syringe touches to the lid of the jar..right?

So you suggest to wipe because it doe snot kill what's inside the needle? Yeah..I was aware that flame does..that's why I was very reluctant to flame after every one.

Thanks.


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A man that comes to the door is never quite the same man who went out.

Freedom is something that dies unless it's used


Edited by newmodel (06/29/05 03:15 PM)


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Offlinepcubmycol
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Re: Syringes and inoculation [Re: newmodel]
    #4350683 - 06/29/05 03:14 PM (12 years, 2 months ago)

yes


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InvisibleLeftyBurnz
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Re: Syringes and inoculation [Re: pcubmycol]
    #4350706 - 06/29/05 03:20 PM (12 years, 2 months ago)

so i take it since i havent seen any contams yet that i got lucky, because i flame sterilized the needles but did not alcohol wipe between each jar, but i did it in my own handcrafted "clean box" and made sure that i sprayed lysol into the box every time my hand left and went back in and i had my friend spray it occasionally around and above the box while i inoculated.


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InvisibleThumpaCap
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Re: Syringes and inoculation [Re: newmodel]
    #4350734 - 06/29/05 03:26 PM (12 years, 2 months ago)

yea its okay to put the needle all the way in ...and be sure to squirt to the inside jar side surface (so you can see the growth start)... but i dont flame sterilize cause if it comes in its own sealed package there is no need. i just make dam sure not to touch the needle or touch it against anything other than the hole in the jar lid (which is covered by med tape and also sterile)..... and i have nnocc'd many many jars without any contams. .. if you need a ghetto cleanroom you can get some plastic sheeting, hang it teepee style from the ceiling and drape it over a table.. and spray the inside with lysol.. and try to be as still and fast as possible when nnocc'ing


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Offlinenewmodel
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Re: Syringes and inoculation [Re: ThumpaCap]
    #4350774 - 06/29/05 03:39 PM (12 years, 2 months ago)

Haha...yeah. My friend that we are cultivating with is a freak about building so..he is making an entire clean box out of plexi--glass. He is also installing the rubber gloves so you have a very clean-clean box.

Thanks all.


--------------------
A man that comes to the door is never quite the same man who went out.

Freedom is something that dies unless it's used


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InvisibleLeftyBurnz
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Re: Syringes and inoculation [Re: newmodel]
    #4350896 - 06/29/05 04:19 PM (12 years, 2 months ago)

mine took me a couple minutes to make, i just completely sealed a cardboard box with duct tape and taped saran wrap over the entrance leaving just enough room to fit the jars, syringes, stuff for a spore print, whatever i need fits, i spray the hell out of the inside before i go to work and spray occasionally in the air above and around it(lysol) it works hella good and didnt cost me a dime.


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InvisibleThumpaCap
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Re: Syringes and inoculation [Re: LeftyBurnz]
    #4352024 - 06/29/05 08:44 PM (12 years, 2 months ago)

that's what i did the first time .... but i taped saran wrap to the top for a window and put a flip door in the front that i could raise up and stick my hands in.. worked very well and hardly ever got contams


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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