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Offlineoptic
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Registered: 06/02/05
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Last seen: 12 years, 7 days
Liquid culture or spore print? Which one is better?
    #4348109 - 06/28/05 10:14 PM (12 years, 3 months ago)

I will soon have twenty 10ml syringes to fill. I need to make a choice on how to fill them. I have the choice to fill them with a few spore prints or do a LC (baby bottle method).

The problem is that I have never done either before. Which method would you recommend to someone just getting started? The syringes will all be used within a month. There is no need for them to last 6 months, etc...

p.s. - Could the LC be started with a chunk of brf/verm cake? If so, could someone point me in the right direction to starting a LC with a small chunk of cake? Thanks...


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Invisibletahoe
Noob Slayer
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Registered: 11/27/03
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Re: Liquid culture or spore print? Which one is better? [Re: optic]
    #4348121 - 06/28/05 10:17 PM (12 years, 3 months ago)

with 1/4th cc of spore water you can make unlimited amount of lc. Fill half with lc's.
lc's are better


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


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OfflineLengthyFellow
I play with poo

Registered: 12/03/04
Posts: 362
Last seen: 8 years, 6 months
Re: Liquid culture or spore print? Which one is better? [Re: optic]
    #4348130 - 06/28/05 10:19 PM (12 years, 3 months ago)

Are you doing different strains? If not just make one syringe with one print, and with 1cc or so make a quart-sized LC with that. If you can use it all within a month then you are a serious cultivator.


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Offlineoptic
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Re: Liquid culture or spore print? Which one is better? [Re: LengthyFellow]
    #4348136 - 06/28/05 10:22 PM (12 years, 3 months ago)

3 strains... Pesa, B+ and creeper.


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OfflineLengthyFellow
I play with poo

Registered: 12/03/04
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Re: Liquid culture or spore print? Which one is better? [Re: optic]
    #4348145 - 06/28/05 10:26 PM (12 years, 3 months ago)

If i were you, Id only make 3 syringes. There's really no need for 20 syringes, because you can use one LC to keep refilling a single syringe. Make one dark syringe for each strain, and make a big ol quart LC for each as well. The syringes will last a LONG time in the fridge, and your LCs can be used all month.

*edit just to clarify, you will use 6 syringes total. 3 of them will be your dark spore syringes and 3 will be used for LC :smile: GL


--------------------


Edited by LengthyFellow (06/28/05 10:27 PM)


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Offlineoptic
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Re: Liquid culture or spore print? Which one is better? [Re: LengthyFellow]
    #4348156 - 06/28/05 10:30 PM (12 years, 3 months ago)

*I* don't need that many. However, I have 4 friends moving next month and these are all going away presents.


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Invisibledeanofmean
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Registered: 12/07/02
Posts: 2,017
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Re: Liquid culture or spore print? Which one is better? [Re: LengthyFellow]
    #4348235 - 06/28/05 11:00 PM (12 years, 3 months ago)

no way would i recommend using a whole print for one syringe.
when i started my first print, i germinated a small amount of spores on cardboard, put that in an LC, before i even looked at a syringe, had more then a few successful grows with it, and i still have that same spore print in archive.


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OfflineHotnuts
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Re: Liquid culture or spore print? Which one is better? [Re: deanofmean]
    #4348347 - 06/28/05 11:33 PM (12 years, 3 months ago)

Dean. You were able to grow out spores on cardboard? In a petri i'm guessing? I use it for cloning, but wasn't sure if spores would grow out on it because of it's lack of nutrients.


Edited by hotnutz (06/28/05 11:34 PM)


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OfflineHippieChick
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Re: Liquid culture or spore print? Which one is better? [Re: optic]
    #4348386 - 06/28/05 11:42 PM (12 years, 3 months ago)

I would do a LC. You can start a LC with any live mycelium, as long as you can get it in the LC under sterile conditions. I've used WBS and it works quite well. Transfered a couple little pieces in a glovebox before I did my last g2g.

Good Luck

Peace,Love,Happiness and Harmony.
:heart: Hippie Chick  :mushroom2:


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Offlineoptic
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Re: Liquid culture or spore print? Which one is better? [Re: HippieChick]
    #4348462 - 06/28/05 11:57 PM (12 years, 3 months ago)

Perfect. Thanks once again everyone. :smile:


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Invisibledeanofmean
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Registered: 12/07/02
Posts: 2,017
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Re: Liquid culture or spore print? Which one is better? [Re: Hotnuts]
    #4348615 - 06/29/05 12:22 AM (12 years, 3 months ago)

i had the same Q. a few years ago, got some good answers from the advanced forum.
but, i don't even know where to look for a link now.
but, use the hard cardboard, like from the back of a note pad.
both cube, and cope/pan spores will germinate on it. but, don't grow mush.
when the spores germinate they look kinda like powered sugar.
you can then place the cardboard on agar w/h2o2, or in LC.
the good thing about this tec is, bacteria, and mold spores don't seem to like cardboard.
so its a great way to start a wild or suspect print. :thumbup:


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InvisibleRoadkillM
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Re: Liquid culture or spore print? Which one is better? [Re: optic]
    #4348812 - 06/29/05 01:08 AM (12 years, 3 months ago)

Don't waste the whole spore print on one syringe.


use 1/4" x 1/4" (square) of a print and make 2 syringes.

save the rest of the print...for later.

knock up some jars with the spore syringes...

and use 1 to 2 cc's of a spore syringe to start an LC.

tc


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


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