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OfflineBlackCat0315
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Question about making sporeprints - Updated with pics
    #4342658 - 06/27/05 01:13 PM (11 years, 5 months ago)

Hey, I just made a few spore prints but didn't do it exactly the way I should have. I cut the caps off with a sterilized knife and placed them on pieces of aluminum foil, and put clean glasses over them with a small opening for air. I didn't have any alcohol to sterilize the foil or the glasses. Do you think these prints will be o.k ? Or should I just trash these and make some more. They made some nice prints, so I hate to toss them if there good.

Thanks !

Update:

here's what the prints I made look like...Is that how they should look ?











Edited by BlackCat0315 (06/27/05 05:23 PM)


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Offlinelepiota
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Re: Question about making sporeprints [Re: BlackCat0315]
    #4342662 - 06/27/05 01:14 PM (11 years, 5 months ago)

they will be ok,
hopefully


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Offlinepcubmycol
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Re: Question about making sporeprints [Re: BlackCat0315]
    #4342665 - 06/27/05 01:15 PM (11 years, 5 months ago)

try making an LC out of the prints...


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Offlinethenewguy05
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Re: Question about making sporeprints [Re: pcubmycol]
    #4342677 - 06/27/05 01:17 PM (11 years, 5 months ago)

should be fine.


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OfflineBlackCat0315
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Re: Question about making sporeprints [Re: thenewguy05]
    #4342697 - 06/27/05 01:22 PM (11 years, 5 months ago)

Thanks guys,
I may try a liquid culture with 1 of them and do WBS with the other..after all, Karo and WBS is cheap and it will just be the time involved....and I got plenty of that !

Now I just have to read up on the proper way to make a syringe.

Thanks


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InvisibleDragonaut
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Re: Question about making sporeprints [Re: BlackCat0315]
    #4342729 - 06/27/05 01:32 PM (11 years, 5 months ago)

You should only need 1 spore print for both. There's plenty of spores in a print. Just fill a jar with water, sterilize it, then you can scrape the spores into it inside a glovebox or something. This way you can make plenty of syringes from one print. Your syringes don't have to be super dark to work.

...Just in case you didn't know.


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Offlinepcubmycol
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Re: Question about making sporeprints [Re: Dragonaut]
    #4342921 - 06/27/05 02:26 PM (11 years, 5 months ago)

I have a personal strain (Jade) which is the most potent field mushroom from about 40 fields that i know about here in Florida. I picked about 300 caps from the field one day, put them all on my coffee table, picked the a flat mid sized cap out and printed it. I scraped about half of the print (from a 2" cap) into a solution of 4% karo 96% water.

In 2 weeks, i shook the jar, pulled 15cc into a syringe and innocced 5 pf cakes (for selective breeding and advancing the strain through cultivation) and 5 pint jars of wbs. I didnt have a single contam, and i was obviously not very sterile about the print.

Unless you know alot about agar, id say the best way to get going off what might be a non sterile print is LC. I would avoid multispore inoculation with your prints... but thats just IMO.


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Offlinethenewguy05
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Re: Question about making sporeprints [Re: pcubmycol]
    #4342947 - 06/27/05 02:30 PM (11 years, 5 months ago)

the print won't guarantee you mushrooms that look and have the same potency as the one you picked. when you take spores it only give you that species and strain, not the characteristics of the parent mushroom. a clone in LC is the way to do that


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Offlinepcubmycol
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Re: Question about making sporeprints [Re: thenewguy05]
    #4342965 - 06/27/05 02:33 PM (11 years, 5 months ago)

If your speaking about jade... its not just that one mushroom that i wanted them all to look like. I just noted that i picked that one out because it was the best specimen for a print. I just wanted the strain, i dont need to do a tissue clone, thats not the issue at all.


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Offlinethenewguy05
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Re: Question about making sporeprints [Re: pcubmycol]
    #4342979 - 06/27/05 02:36 PM (11 years, 5 months ago)

gotcha. strain...


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OfflineBlackCat0315
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Re: Question about making sporeprints [Re: thenewguy05]
    #4343001 - 06/27/05 02:41 PM (11 years, 5 months ago)

Hey there,
thanks for all the good information here. This is a great forum...always good people to help out someone in need. I'm definately gonna try to do something with these prints (mexi-cubs) and if they don't work, I've got some PES Hawaiians starting to flush again...I'll just get the alcohol this time and do it by the book.

Thanks again for your help !


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Offlinepcubmycol
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Re: Question about making sporeprints [Re: thenewguy05]
    #4343028 - 06/27/05 02:46 PM (11 years, 5 months ago)

Quote:

thenewguy05 said:
gotcha. strain...




:thumbup: :thumbup:

and what a badass strain it is. Its currently in the second generation. Believe ir or not, it colonizes at the exact same speed that KS does!!! I usually get about a 9-10 day colonization for half pints(pf style) knocked up with 1cc of LC per jar in optimal conditions. One colonized 100% in 8 days! Thats my record for KS too. The Mycelium of jade is very rhizomorphic and seems to be fairly resistant to contams. I get about the same trip on 1.5 grams of jade as i do on 1 gram of KS, or 3 grams of B+! (Of course the trips arent exactly the same)

The point is, once i perfect this strain (about 2 months), ill be sending out prints to 3 people who want to experiment with it, that i can trust.


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InvisibleRoadkill
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Re: Question about making sporeprints [Re: pcubmycol]
    #4343133 - 06/27/05 03:09 PM (11 years, 5 months ago)

I have a South American isolate that is faster.
:smirk:

I can colonize 1/2 pint jars of BRF/Verm with a multi spore inoculation and have full colonization in 7 to 8 days.

With a LC....5 to 6 days.

and they fruit in 4 days...after dunking and cold shocking...for 12 hours.

nothing is faster...in my book.

I'll test out your "Jade" strain...I'm always interested in experimenting with new stuff.

tc


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Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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Offlinepcubmycol
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Re: Question about making sporeprints [Re: Roadkill]
    #4343191 - 06/27/05 03:23 PM (11 years, 5 months ago)

RK, you'll be the first one i send it to. I havent thrown it into bulk yet, but i know you like the poo, im sure it will be the shit. Like i said, i want to go ahead and get it into a 3rd gen, ill be sending out the 3 prints after i do my next run of millet based casings!


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InvisibleRoadkill
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Re: Question about making sporeprints [Re: pcubmycol]
    #4343198 - 06/27/05 03:24 PM (11 years, 5 months ago)

thank you!~

I look forward to it.

:smile:


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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OfflineBlackCat0315
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Re: Question about making sporeprints [Re: Roadkill]
    #4343717 - 06/27/05 05:31 PM (11 years, 5 months ago)

Updated with pics...Is this how they should look, and is it ok to store these in a zip lock baggie ?


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Offlinepcubmycol
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Re: Question about making sporeprints [Re: BlackCat0315]
    #4343734 - 06/27/05 05:34 PM (11 years, 5 months ago)

those loook very nice, i usually sterilize a double sized layer of foil, and fold one end over the other before it goes into the plastic baggie


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OfflineBlackCat0315
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Re: Question about making sporeprints [Re: pcubmycol]
    #4343744 - 06/27/05 05:36 PM (11 years, 5 months ago)

Well I forgot to sterilize the foil, but I did fold the extra foil over the prints before I put them in the baggie. Hope that works !


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Offlinepcubmycol
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Re: Question about making sporeprints [Re: BlackCat0315]
    #4343795 - 06/27/05 05:45 PM (11 years, 5 months ago)

again, id go for LC here. You can do something small, like 25ml water 1ml karo in 4oz jars (or 1/2 pint if thats all you have). Try to make like, 4 small LCs off 1 print, and a few syringes off the other... be as sterile as possible the rest of the way, germ mask, hair net and all.

Next time, just put a plate with foil on it and a bowl upside down covering the foil in the oven. 275 degrees for an hour. Let cool for 4 or 5 hours. Place cap in on foil as fast as possible and put back in oven. Let sit for a couple days... print made.


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