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OfflineBlue Helix
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Questions about storing liquid cultures.
    #4336183 - 06/25/05 04:10 PM (11 years, 3 months ago)

My first liquid culture is progressing very rapidly. I am 48 hours into the culture and it's already snowing in there pretty heavy. At this rate, I think it will be ready to use in another three or four days. I'll have way too much to use really, so I have to store some of it to seed the next jar.

Obviously, it would be a lot easier and the LC would be subject to less contamination risk if I collect the LC full strength sugar solution and store it like that, but how bad is it if one stores the LC in refrigerated vaccutainers without reducing the sugar level? Specifically, does anyone have any idea how long will the culture keep like that? How much longer will it keep if stored in sterile water? Does anyone direct experience or have a friend with experience with long term storage in the full-strength 4% sugar solution?

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Re: Questions about storing liquid cultures. [Re: Blue Helix]
    #4336640 - 06/25/05 06:46 PM (11 years, 3 months ago)

If the plan is to store it for a few weeks, or a month, simply swab down the jar with alc, allow it to evap, cling wrap the jar (well)& place it in the fridge. 40F will stall all growth.


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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