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OfflineAnything4Poppies
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Registered: 01/07/05
Posts: 145
Last seen: 9 years, 7 months
Been a while since serious post... QUESTION LC
    #4335025 - 06/25/05 04:45 AM (11 years, 5 months ago)

OKay so would it be possible in a sterile environment to scrape a part of a print into a karo solution and consider it hydrated? This would be convenient, so anyone try this? By this i mean eliminating the syringe step and hoping that just sitting in solution would hydrate like water would before injection. Also my logs from before I am going to attempt to pack them with pasteurized straw / verm and inject them in several places with liquid myc.

Thanks again as always

---A4P


--------------------
"What a long, strange trip it's been" --- GD
"I think, Therefore I am." --- Rene Descartes


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Invisibleagar
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Registered: 11/21/04
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Re: Been a while since serious post... QUESTION LC [Re: Anything4Poppies]
    #4335064 - 06/25/05 04:59 AM (11 years, 5 months ago)

If you place dehydrated spores into a solution, they will begin to hydrate on contact with the solution & normaly begin to germinate within a few days, if the tempurature is in the proper range (roughly 72 to 84F).

Any liquid is a contaminate magnet. What you suggest should be done in a glove box. Opening a print in less than a very sanitized draft free area, is a possible contamination vector, same as opening any LC. All it takes is a sorry little mold spore to F/U the works.

So - be as sanitary - as possible.


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OfflineAnything4Poppies
Wanderer Who HasCome Home

Registered: 01/07/05
Posts: 145
Last seen: 9 years, 7 months
Re: Been a while since serious post... QUESTION LC [Re: agar]
    #4335082 - 06/25/05 05:06 AM (11 years, 5 months ago)

so then the needle syringe altough adding a step ups the sanitation in the transfer? I dont have any needles right now as I cannot find / buy one so Im thining i need suggestion on print ---> LC ... i see contam issue... ive been okay sucessful in the past but do not have a glove box. do have clean though. Thanks for the response.

---A4P


--------------------
"What a long, strange trip it's been" --- GD
"I think, Therefore I am." --- Rene Descartes


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OfflineAnything4Poppies
Wanderer Who HasCome Home

Registered: 01/07/05
Posts: 145
Last seen: 9 years, 7 months
Re: Been a while since serious post... QUESTION LC [Re: Anything4Poppies]
    #4336277 - 06/25/05 05:02 PM (11 years, 5 months ago)

bump... just once for more insight


--------------------
"What a long, strange trip it's been" --- GD
"I think, Therefore I am." --- Rene Descartes


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