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OfflineBlue Helix
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White stuff above top of LC. Is this contamination?
    #4333802 - 06/24/05 10:09 PM (11 years, 6 months ago)

This is a 4% malt extract+dextrose LC. What is the white stuff right above the water line? Anyone ever see something like this? Is it contaminated?



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InvisibleHolydiver
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Re: White stuff above top of LC. Is this contamination? [Re: Blue Helix]
    #4333808 - 06/24/05 10:14 PM (11 years, 6 months ago)

I can't make out anything in the picture.


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Re: White stuff above top of LC. Is this contamination? [Re: Holydiver]
    #4333811 - 06/24/05 10:15 PM (11 years, 6 months ago)

HUH? You can't make out anything? You don't see the white stuff above the LC?


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Re: White stuff above top of LC. Is this contamination? [Re: Blue Helix]
    #4333814 - 06/24/05 10:17 PM (11 years, 6 months ago)

I see something white, yes. What it represents physically, I can't tell. Is it floating? Is it rising up the sides of the glass? If so, that's just from the LC solution evaporating, and I've seen it before.


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Re: White stuff above top of LC. Is this contamination? [Re: Blue Helix]
    #4333819 - 06/24/05 10:19 PM (11 years, 6 months ago)

The white stuff look harmless to me, but this is my first LC so I haven't a clue. It looks like some sort of white residue that has formed on the sides of the glass.


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Re: White stuff above top of LC. Is this contamination? [Re: Blue Helix]
    #4333822 - 06/24/05 10:20 PM (11 years, 6 months ago)

Ok, then it's evaporation from the LC solution and not a contamination.


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Re: White stuff above top of LC. Is this contamination? [Re: Holydiver]
    #4333839 - 06/24/05 10:29 PM (11 years, 6 months ago)

It looks like evaporated malt dextrose


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Re: White stuff above top of LC. Is this contamination? [Re: Blue Helix]
    #4333860 - 06/24/05 10:34 PM (11 years, 6 months ago)

Thanks. I believe it is probably evaporated malt. Just keeping an eye on it.


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Re: White stuff above top of LC. Is this contamination? [Re: Blue Helix]
    #4333904 - 06/24/05 10:42 PM (11 years, 6 months ago)

It would appear to me to be some sort of residue from the malt/dextrose mix. Never seem that before. But, I doubt it will do any damage & isn't a contaminate. The white I get is myc floating on top the solution, as the culture gets going - well.


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Re: White stuff above top of LC. Is this contamination? [Re: agar]
    #4333978 - 06/24/05 11:00 PM (11 years, 6 months ago)

It might be due to the type of malt I used. My brew supply called it extra light malt and it was as fine as flour. The brewer claimed it was the best malt available for any price, but I don't know if what is "best" for beer is best for mycelium. The regular light malt I used to use with agar was different. Also, the mixer heats the solution to 84F while the room is only 78F. The is a side effect of the relatively low efficiency of the mixer.


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Re: White stuff above top of LC. Is this contamination? [Re: Blue Helix]
    #4336409 - 06/25/05 05:37 PM (11 years, 6 months ago)

Here the culture is today. It seems to be developing more "dead skin" stringy stuff. I never see this in the pictures of LC on here. Has anyone seen this before?



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Re: White stuff above top of LC. Is this contamination? [Re: Blue Helix]
    #4336813 - 06/25/05 07:25 PM (11 years, 6 months ago)

This entire thread has just been explained to me by agar:

You see I thought the idea was to stir the liquid culture 24/7 with a magnetic stirrer for best results. That is what I have been doing too. I guess most only stir their LC every day or a couple times a day. That misunderstanding caused the evaporated malt stuff on the sides and the unusual mycelium growth I am sure.

Thanks everyone for the input.


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Re: White stuff above top of LC. Is this contamination? [Re: Blue Helix]
    #4336859 - 06/25/05 07:43 PM (11 years, 6 months ago)

In the book Growing Medicinal and Gourmet Mushrooms:

"Each Erlenmeyer is placed on stir plates or on a shaker table and rotated at 100-200 rpm for 48-72 hours. The water broth is continuously stirred to allow for transpiration of metabolic gases and for oxygen absorption. The fluid has a milky brown color and is not translucent. Settling of the heavier components is clearly visible when the stirring process is interrupted."

He goes on to say:

"After two to four days of re-growth in the nutrient enriched broth, each Erlenmeyer flask becomes its own universe, hosting thousands of stellar-shaped, three dimensional colonies of mycelium. This is the stage ideal for inoculation into sterilized substrates, especially in the generation of grain spawn masters."

He seems a little weak on his math here as 48-72 hours would equate to 2-3 days, not 2-4 but I get the idea. I am seeing the stellar-shaped colonies he talks about and am the only person around here doing things the way that Stamets recommends in his book. I'll just keep stirring it 24-7 for the next day or two and use it. That cuts my time down a few days over the more traditional techniques outlined on the Shroomery which is cool. It just looks kinda different in the culture.


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Re: White stuff above top of LC. Is this contamination? [Re: Blue Helix]
    #4336935 - 06/25/05 08:16 PM (11 years, 6 months ago)

I shake mine by hand twice a day, and they turned out looking like that...


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Re: White stuff above top of LC. Is this contamination? [Re: Blue Helix]
    #4336960 - 06/25/05 08:28 PM (11 years, 6 months ago)

The first LC's I did, I did not have a stir plate. I read Stamets & disagree. (LOL, on this and another thing)).

IMHO - you get better growth in a still culture. Ask yourself, how well would any culture grow, in a high speed liquid vortex.

Once you have growth, using a stir plate (once a day for 15 minutes), whips that growth to tiny shreds. Those tiny shreds each grow. That multiplies the tissue in the culture - exponentially.







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Re: White stuff above top of LC. Is this contamination? [Re: agar]
    #4336977 - 06/25/05 08:35 PM (11 years, 6 months ago)

I will definitely try both techniques and see what I think for myself. So far, man, this LC thing is like some sort of mycologist's wet dream! I have never seen anything so easy with such a high rate of return for almost no investment whatsoever. I cannot imagine why anyone would use a spore syringe straight from a vendor with something this easy to help them. I got it with agar since agar is a pain in the ass, but this LC stuff is painless as can be--at least so far... ask me when it's all done if I still lean that way.


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Re: White stuff above top of LC. Is this contamination? [Re: Blue Helix]
    #4337009 - 06/25/05 08:53 PM (11 years, 6 months ago)

The older hands use LC's - just for the reasons you mention. You can propogate X 1,000,000,000 very easy. Once you learn to store them, you never need to buy a spore syringe. Unless it is a strain you don't have.

One reason LC's are not shouted about - to much. Is that they are live cultures. So, you should not sell, or mail them. As - that could get you in hot water, should shit happen. Not true - with spore prints, or spore syringes.

If LC's were legal, spore vending would not be a profitable thing. As, LC's are easy & cheap to make & multiply. The other advantage of an LC, is you can tell - if one is contaminated, if you take the time with it. That is not true of a print, or syringe - until you put them to use.


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Re: White stuff above top of LC. Is this contamination? [Re: agar]
    #4337021 - 06/25/05 08:57 PM (11 years, 6 months ago)

Agar, what's the longest you've kept an LC in the fridge and used it and it was still viable?


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Re: White stuff above top of LC. Is this contamination? [Re: Blue Helix]
    #4337063 - 06/25/05 09:16 PM (11 years, 6 months ago)

Personally, I wouldn't mail spores as a vendor in the US for any reason.  It's one of those "on the edge" ways to make money, and if the policial climate continues to go south, you know who's door they'll be knocking.  Radical right wingers love to destroy the lives of those who have "cheated" to make money; it's a form of justice in their mind since making money should be, in their belief system, evidence of a "good, hardworking person" not some cheater or loafer.  After all life is only about making money and keeping as much as possible, right?  :rolleyes:  Oh, and the more money you make or the more famous you happen to be, the more danger you are in if you flirt on the edge with these guys.  Just look at Tommy Chong's life if you want to know what I mean...


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Re: White stuff above top of LC. Is this contamination? [Re: Blue Helix]
    #4337075 - 06/25/05 09:25 PM (11 years, 6 months ago)

Anyways, how about those LC's? (Keep it on topic please)


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Amazon Shop for: Agar, Magnetic Stirrer, Malt Extract, Paul Stamets

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