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Offlinelardnar
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EDIT: No more tams, good growth, go lipa yai!
    #4333530 - 06/24/05 08:26 PM (12 years, 4 days ago)

Hey guys,

OK I found out why my 10 Pink Buffalo jars might not be colonizing. I purchased a terrarium humidity sensor and it read that my rH was 34! These jars had been in this environment from last Sunday night to this morning. It looks like the verm against the sides are still moist but I can't be sure. The BRF hasnt turned the light color it was when it came out of the package from what i can see from the glass. Do you think these will still be salvageable?

I started two new jars today. Pressure cooked em for 30 minutes, let em cool for 3+ hours and then inoculated them with Lipa Yai. I now have all 12 of the jars in a terrarium I built that holds at 70% rH.

The temps are from the mid 70's (at night) to 80 (during the day), do you think the first 10 will survive?


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If your soul is sence this life is lost ...


Edited by lardnar (06/30/05 04:44 PM)


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InvisibleHolydiver
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Re: Low humidity jars, can they come back? [Re: lardnar]
    #4333540 - 06/24/05 08:29 PM (12 years, 4 days ago)

External humidity has nothing to do with jars colonizing. Substrate moisture, content, and temperature DO however play a major role in colonization. You worry about humidity later on, when it's time to fruit. Temps sound a bit low.


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To find a place to live between the negatives and positives.


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Offlinelardnar
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Re: Low humidity jars, can they come back? [Re: Holydiver]
    #4333553 - 06/24/05 08:33 PM (12 years, 4 days ago)

OK cool. What I did for each 2 jars was take a cup of coarse verm and do the wet in bowl until it cant hold anymore water then dump the excess water, then mixed with a half cup of BRF. I also did the half inch of verm.

I thought since the holes on my jars are uncovered they could lose a decent amount of moisture through them, especially the way verm likes water i thought it could wick it up to the holes and let it evaporate. They were not in a terrarium, they were open in the closet.


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InvisibleHolydiver
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Re: Low humidity jars, can they come back? [Re: lardnar]
    #4333557 - 06/24/05 08:35 PM (12 years, 4 days ago)

As long as you have a dry verm barrier, the holes won't cause your substrate to evaporate too much. The little bit or air exchange is helpful. I don't recommend taping over holes for that reason.

Far as the mixture goes, can't help you there...I haven't mixed a PF cake in years.


--------------------
To find a place to live between the negatives and positives.


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Offlinelardnar
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Re: Low humidity jars, can they come back? [Re: Holydiver]
    #4334478 - 06/25/05 01:25 AM (12 years, 3 days ago)

What do you think is holding my cakes back?

I was just estimating temps before, its actually low to mid 80's in here (i'm sweatin)

BTW thanks for the help, if it wasn't for you guys' reassurance i would have 36 jars goin now pullin my hair out


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If your soul is sence this life is lost ...


Edited by lardnar (06/25/05 01:26 AM)


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Offlinelardnar
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Re: Low humidity jars, can they come back? [Re: lardnar]
    #4342560 - 06/27/05 12:50 PM (12 years, 1 day ago)

OK, here's the update!



The pink buffalo's were inoculated 8 days ago and the Lipa Yai 3 days ago. They have since been in a terrarium at 80-83 degrees and 70% rh

Break down: (PB = Pink Buffalo)
PB1: 2 marks of colonization (pictured)
PB2: nothing
PB3: 1 mark
PB4: 2 marks
PB5: 1 mark
PB6: nothing
PB7: 1 mark
PB8: 1 mark
PB9: nothing
PB10: 1 mark plus strange redding pictured below around what might be another

Lipa Yai1: None
Lipa Yai2: None

Here is the strange redding around one of the inoculation sites that has a tiny bit of colonization in the middle:



What does this mean?? This is the only jar with any sign of something possibly being wrong (besides long colonization times)

Many thanks to the shroomery members and mods for making this possible!

Moral of the story: New growers don't get too worried if your jars don't colonize the first week, even if in the right conditions


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If your soul is sence this life is lost ...


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Offlinelardnar
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Re: Low humidity jars, can they come back? [Re: lardnar]
    #4342736 - 06/27/05 01:33 PM (12 years, 1 day ago)

Anyone with ideas on the red/orange?


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Invisibleohmatic
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Re: Low humidity jars, can they come back? [Re: lardnar]
    #4342743 - 06/27/05 01:34 PM (12 years, 1 day ago)

looks like mold to me, ive seen red/orange mold couple times before.
toss it.
peace ohm :mushroom2:


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:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !


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Offlinelardnar
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Re: Low humidity jars, can they come back? [Re: ohmatic]
    #4342770 - 06/27/05 01:42 PM (12 years, 1 day ago)

Can anyone confirm? If so she gets the big toss! (well i'll dump it outside and see what happen)


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Invisibleagar
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Re: Low humidity jars, can they come back? [Re: lardnar]
    #4343114 - 06/27/05 03:04 PM (12 years, 1 day ago)

Trash can tek that puppy with the red/brown crap in it.

Picture isnt good enough to identify the mold specificaly.

Whatever it is, it ISN'T A GOOD THING.
Read below
~~~~~~~~~~~~~~~~~~~~~~~~

Cinnamon Brown Mold ? Chromelosporium fulva (Peziza ostrachoderma)
The color of this mold ranges from yellow gold to golden brown to cinnamon brown. It grows rapidly in circular patches. It is very common in soil, and flourishes on damp wood. Areas in compost overheated during spawn run may be colonized. Improperly conditioned compost will also support growth, but it is most commonly known as a recolonizer of overly pasteurized casing, possibly living on dead microorganisms. It often occurs on sterilized soil. Sexual fruiting bodies may appear several weeks after the first appearance of the mold. Spores are airborne.


Lipstick Mold ? Sporendonema purpurescens (Geotrichum candidium)
This fungus colonizes compost or casing. As spores mature, the color of the mold changes from white to pink, to cherry red, and finally to dull orange. It is slow growing. Spores spread in air, during watering, and on pickers. The lipstick mold utilizes certain fats in the compost. It is an uncommon problem. Control is centered around sanitation.


Pink Mold; Red Bread Mold ? Neurospora
Commonly to occasionally seen on agar and grain. Neurospora is fast growing, sometimes taking only 24 four hours to totally colonize a media filled petri dish. It is ubiquitous in nature, occurring on dung, in soils and on decaying plant matter. Since this fungus grows through cotton stoppers or filter discs, a single contaminated jar, though sealed, can spread spores to adjacent spawn jars within the laboratory. This condition is more likely if the filter discs or cotton plugs are the least bit damp; or if the external humidity is high. Furthermore, Neurospora spores germinate more readily at elevated temperatures. The pink mold seen in mushroom culture is most frequently Neurospora sitophila, a pernicious contaminant that is difficult to eliminate. All infected cultures should be removed as soon as possible from the laboratory and destroyed. A thorough cleaning of the laboratory is absolutely necessary. If contamination persists, remove all spawn and start anew.


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Offlinelardnar
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Re: Low humidity jars, can they come back? [Re: agar]
    #4343159 - 06/27/05 03:15 PM (12 years, 1 day ago)

Many many thanks!


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Offlinescatmanrav
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Re: Low humidity jars, can they come back? [Re: lardnar]
    #4343849 - 06/27/05 05:57 PM (12 years, 1 day ago)

Looks like rust to me, I'd keep it and see if it spreads...if it does (or you can tell its not rust), trash it.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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Offlinelardnar
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Re: Low humidity jars, can they come back? [Re: scatmanrav]
    #4343931 - 06/27/05 06:13 PM (12 years, 1 day ago)

Definately not rust, I crawl under rusty sports cars all day

That jar is long gone now, in a dumpster miles away


--------------------
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Offlinelardnar
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Contams! [Re: lardnar]
    #4346248 - 06/28/05 10:44 AM (12 years, 13 hours ago)

I lost the two adjacent jars to this contamination also. There was no sign of it yesterday but a little today. They got the boot, don't wanna risk further spreading.

No more jars have germinated, lost one of the jars that was


--------------------
If your soul is sence this life is lost ...


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InvisibleRoadkill
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Re: Contams! [Re: lardnar]
    #4346259 - 06/28/05 10:53 AM (12 years, 13 hours ago)

was the color red or orange?

I can't tell from the picture...my eyes are getting bad with old age.
:frown:


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Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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Offlinelardnar
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Re: Contams! [Re: Roadkill]
    #4346357 - 06/28/05 11:54 AM (12 years, 12 hours ago)

its more of an orange actually


--------------------
If your soul is sence this life is lost ...


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OfflineMaJikFungus
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Re: Contams! [Re: lardnar]
    #4346389 - 06/28/05 12:17 PM (12 years, 12 hours ago)

I had the same crap in the first set of jars I did. Deffinatly contam , only lost one jar to it though. Does yours look like this?



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Offlinelardnar
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Re: Contams! [Re: MaJikFungus]
    #4346467 - 06/28/05 01:00 PM (12 years, 11 hours ago)

yeah, it's that color but much less concentrated. Kinda bums me out that I only have 6 colonized jars left but I have 3 more that should germinate any day


--------------------
If your soul is sence this life is lost ...


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Offlinepcubmycol
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Re: Low humidity jars, can they come back? [Re: Holydiver]
    #4346530 - 06/28/05 01:25 PM (12 years, 11 hours ago)

Quote:

Diver said:
External humidity has nothing to do with jars colonizing. Substrate moisture, content, and temperature DO however play a major role in colonization. You worry about humidity later on, when it's time to fruit. Temps sound a bit low.




External humidity can play a factor in this. Having an rH of 34% sounds like it could dry your cakes out, but your incubation chamber will be contam free. It doesnt make a big difference, but i think its noticable if your incubator has an rH of between 70 and 80% that is optimal, so things dont dry out, but its still hard for contaminants to get a foothold...

Also, raise your temps up to a constant between 84 and 86, but do not exceed 86. Read about TiT (tub in tub) or a reptile pad heater with towels over it...


--------------------
weretheshit.com is the shit!

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OfflineHotnuts
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Re: Low humidity jars, can they come back? [Re: pcubmycol]
    #4346589 - 06/28/05 01:45 PM (12 years, 10 hours ago)

Sounds like Lipstick to me. Lipstick starts out white like trich and after it starts to sporulate, then it darkens to red and then on to orange. Could be cinnamon too. No matter what mold it is, the jar's toast.


Edited by hotnutz (06/28/05 01:46 PM)


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