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Dimitrion
Apprentice
Registered: 06/24/05
Posts: 16
Loc: The Netherlands
Last seen: 18 years, 3 months
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It won't spawn!
#4331335 - 06/24/05 03:32 AM (18 years, 9 months ago) |
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I've inoculated 4 jars on 18-06-05. Now it's 24-06 and NOTHING has happened.
I used BRF and vermiculite. Pressure Cooked the jars. Inoculated the jars on 4 different points, sterilizing the needle between each inoculation. The jars are stored in a terrarium at 27 degrees Celsius, out of direct sunlight. The strain used is Psilocybe cubensis "Matias Romero".
Is it possible that there is to little humidity INSIDE the jars?
-------------------- I will make this happen!
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lepiota
expert infailures
Registered: 10/27/04
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Loc: Europe
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Re: It won't spawn! [Re: Dimitrion]
#4331339 - 06/24/05 03:37 AM (18 years, 9 months ago) |
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Quote:
Dimitrion said: I used BRF and vermiculite.
no water?
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LengthyFellow
I play with poo
Registered: 12/03/04
Posts: 362
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Re: It won't spawn! [Re: lepiota]
#4331353 - 06/24/05 03:57 AM (18 years, 9 months ago) |
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Did you wait for the jars to cool COMPLETELY before you innocd? this can take a long time (like 5-8 hours)
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Prisoner#1
Even Dumber ThanAdvertized!
Registered: 01/22/03
Posts: 193,665
Loc: Pvt. Pubfag NutSuck
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Re: It won't spawn! [Re: Dimitrion]
#4331386 - 06/24/05 05:06 AM (18 years, 9 months ago) |
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one of the jars is showing signs of colonization, be patient
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Dimitrion
Apprentice
Registered: 06/24/05
Posts: 16
Loc: The Netherlands
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Re: It won't spawn! [Re: lepiota]
#4331438 - 06/24/05 06:19 AM (18 years, 9 months ago) |
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Quote:
lepiota said:
Quote:
Dimitrion said: I used BRF and vermiculite.
no water?
Yes, i did use water. Just enough to make it drip a little bit when squeesed.
-------------------- I will make this happen!
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Kalix
'Head
Registered: 03/20/05
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Re: It won't spawn! [Re: Prisoner#1]
#4331440 - 06/24/05 06:20 AM (18 years, 9 months ago) |
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Quote:
Prisoner#1 said: one of the jars is showing signs of colonization, be patient
-------------------- My Unitarian Jihad Name is: The Shotgun of Sweet Reason
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Dimitrion
Apprentice
Registered: 06/24/05
Posts: 16
Loc: The Netherlands
Last seen: 18 years, 3 months
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Quote:
LengthyFellow said: Did you wait for the jars to cool COMPLETELY before you innocd? this can take a long time (like 5-8 hours)
I think i did, I waited a whole night and day before inoculation. That would be long enough. i guess?
-------------------- I will make this happen!
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Dimitrion
Apprentice
Registered: 06/24/05
Posts: 16
Loc: The Netherlands
Last seen: 18 years, 3 months
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Re: It won't spawn! [Re: Prisoner#1]
#4331446 - 06/24/05 06:25 AM (18 years, 9 months ago) |
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Quote:
Prisoner#1 said: one of the jars is showing signs of colonization, be patient
Really? What are signs of inoculation? I don't see white fuzz or anything like it, do you? The picture aint that good
-------------------- I will make this happen!
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Kalix
'Head
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Re: It won't spawn! [Re: Dimitrion]
#4331453 - 06/24/05 06:33 AM (18 years, 9 months ago) |
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I don't see anything that 100% shows signs of colonization, but you have to wait @ least a few more weeks..
-------------------- My Unitarian Jihad Name is: The Shotgun of Sweet Reason
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Dimitrion
Apprentice
Registered: 06/24/05
Posts: 16
Loc: The Netherlands
Last seen: 18 years, 3 months
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Re: It won't spawn! [Re: Kalix]
#4331455 - 06/24/05 06:37 AM (18 years, 9 months ago) |
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Quote:
Kalix said: I don't see anything that 100% shows signs of colonization, but you have to wait @ least a few more weeks..
Oh oke, I'll work on my patience So there is no need to ad water to the jars?
-------------------- I will make this happen!
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lordoftheshroomz
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Re: It won't spawn! [Re: Dimitrion]
#4331465 - 06/24/05 06:43 AM (18 years, 9 months ago) |
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no need to ad water to the jars... but also no need to wait a few weeks... more like a few days before u should see ure first signs of mycelium! but maybe im retarded from all the dxm ive been doing lately! who knows
-------------------- MY Glove Box, Automated Terrarium, and Incubator Lil' Wyte, Three Six Mafia, Project Pat will bring it to ya door if theres ana on ya chest. THE PATRIOT ACT MAY BE THE DOOM OF US ALL! THEY CAN GET THINGS LIKE YOUR MEDICAL/LEGAL RECORDS, YOUR INTERNET HISTORY, YOUR GOOGLE SEARCH INFORMATION, ALL WITHOUT PROBABLE CAUSE OR A WARRANT! ALSO NO ONE CAN LEGALLY TELL YOU. YOU'LL NEVER KNOW UNTIL IT'S TOO LATE, THIS ISN'T EXACTLY A FREE COUNTRY ANYMORE NOW IS IT? THEY ARE DESTROYING THIS COUNTRY SO THEY CAN GET RICHER! THE WAR IN IRAQ ACCOMPLISHED NOTHING! HELP GET THE CONSERVATIVE RIGHT WING-NUTS OUT OF POWER, MAKE AN EDUCATED VOTE!
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scatmanrav
Brainy Smurf
Registered: 05/08/04
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See the spot circled in red....right under the coffee filter theres some colonization it looks like.
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QuadrupleTree
Single Whip
Registered: 05/09/05
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Re: It won't spawn! [Re: scatmanrav]
#4331677 - 06/24/05 09:18 AM (18 years, 9 months ago) |
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no i think that's foil
-------------------- I don't know if you are ready, but I'd like to offer the Truth of Dr. David R. Hawkins...http://www.veritaspub.com People talk about getting "the fear." There is simply a lack of inner confidence and comfort. Those people haven't studied any type of mind training. I suggest they seriously consider Qigong.
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backupwards
peon
Registered: 04/02/05
Posts: 3,022
Loc: somewhere else
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its some kind of plastic wrap or something under that. why is that there? maybe foil but looks like plastic to me. 6 days is not a long time to have waited, give it a little more time before you worry. peace
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WillieTomg
If stemmer votedme 1, I'm doingSOMETHING right!
Registered: 04/09/05
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My first jars took 4 weeks to colonize.
When we say "patience is a virtue" we aren't kidding. Sometimes it takes only a few days, sometimes it takes several weeks on end. The jars will be done when they're done, and not a second sooner. Have faith my brother...
-------------------- Battles of wits are impossible with the unarmed.
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grow
i ^
Registered: 04/09/05
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Re: It won't spawn! [Re: Dimitrion]
#4332023 - 06/24/05 10:48 AM (18 years, 9 months ago) |
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I guess I'm the only one who caught on....
wrap your terrarium with a black trash bag immediately. they are not supposed to be exposed to light @ all
where did you get your spores from?
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thenewguy05
The Mushroom Man
Registered: 02/11/05
Posts: 2,123
Loc: My Underground Layer
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Re: It won't spawn! [Re: Dimitrion]
#4332036 - 06/24/05 10:52 AM (18 years, 9 months ago) |
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Quote:
Dimitrion said:
Quote:
lepiota said:
Quote:
Dimitrion said: I used BRF and vermiculite.
no water?
Yes, i did use water. Just enough to make it drip a little bit when squeesed.
you prolly should have stuck to the pf formula. sounds like you put way too much water in there. if you notice in the formula the water content is the same as the rice leaving very little room for ANY water to be sqeezed out of it. pf formula is 1/4 cup brown rice 1/2 cup vermiculite 1/4 cup water
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scatmanrav
Brainy Smurf
Registered: 05/08/04
Posts: 11,483
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Re: It won't spawn! [Re: grow]
#4332323 - 06/24/05 12:08 PM (18 years, 9 months ago) |
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Quote:
grow said: I guess I'm the only one who caught on....
wrap your terrarium with a black trash bag immediately. they are not supposed to be exposed to light @ all
where did you get your spores from?
Actually, Mr. Swift, the only reason no one else mentioned it is because its not a big deal
I still think it looks like mycelium to me I do see how it looks like tape that was peeled off or something now though...thats probably what Anno saw too though.
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prefloppro
Last Call
Registered: 05/29/05
Posts: 440
Last seen: 18 years, 2 months
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Re: It won't spawn! *DELETED* [Re: scatmanrav]
#4332366 - 06/24/05 12:19 PM (18 years, 9 months ago) |
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Post deleted by preflopproReason for deletion: Made a fool out of myself.
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scatmanrav
Brainy Smurf
Registered: 05/08/04
Posts: 11,483
Loc:
Last seen: 11 years, 2 months
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Re: It won't spawn! [Re: prefloppro]
#4332404 - 06/24/05 12:30 PM (18 years, 9 months ago) |
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Quote:
prefloppro said: Not a big deal to keep your jars completly dark? lol. I can personally tell you thatit took me the first time 6 weeks for my jars to colonize because i would look at them every 3 hours or so. My second batch and all new batches take 2 weeks max to 100 colonize, because i leave them alone in complete darkeness, and only look at them once a week. I am 100 percent sure this is why they are colonizing faster. But hey you seem to know it all. Lmao
I dont know it all, but man you want to tell me about colonizing jars?
I mean how are you gonna explain dozens of other people, including myself, who ca get jars to colonize from spores in a week with the light on...or using a light as a source of heat. I'm not gonna argue this with ya buddy, if you want to think thats what it was and not the water content, spores, temperature, air exchange, or any number of the other variables that DO play a role in speed of colonization, then you can keep thinking that. Those who know, know.
By the way, you know the original PF Tek, by Fanaticus, specifically said in the directions to incubate in the light? How do you think all the people who did and do that get along with jars under 2 weeks all the time?
Edited by scatmanrav (06/24/05 12:37 PM)
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