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OfflineDimitrion
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Registered: 06/24/05
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It won't spawn!
    #4331335 - 06/24/05 05:32 AM (11 years, 5 months ago)

I've inoculated 4 jars on 18-06-05.
Now it's 24-06 and NOTHING has happened.

I used BRF and vermiculite. Pressure Cooked the jars. Inoculated the jars on 4 different points, sterilizing the needle between each inoculation.
The jars are stored in a terrarium at 27 degrees Celsius, out of direct sunlight.
The strain used is Psilocybe cubensis "Matias Romero".

Is it possible that there is to little humidity INSIDE the jars?


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Offlinelepiota
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Re: It won't spawn! [Re: Dimitrion]
    #4331339 - 06/24/05 05:37 AM (11 years, 5 months ago)

Quote:

Dimitrion said:
I used BRF and vermiculite.




no water?


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OfflineLengthyFellow
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Re: It won't spawn! [Re: lepiota]
    #4331353 - 06/24/05 05:57 AM (11 years, 5 months ago)

Did you wait for the jars to cool COMPLETELY before you innocd? this can take a long time (like 5-8 hours)


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InvisiblePrisoner#1M
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Re: It won't spawn! [Re: Dimitrion]
    #4331386 - 06/24/05 07:06 AM (11 years, 5 months ago)

one of the jars is showing signs of colonization, be patient


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OfflineDimitrion
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Re: It won't spawn! [Re: lepiota]
    #4331438 - 06/24/05 08:19 AM (11 years, 5 months ago)

Quote:

lepiota said:
Quote:

Dimitrion said:
I used BRF and vermiculite.




no water?




Yes, i did use water. Just enough to make it drip a little bit when squeesed.


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OfflineKalix
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Re: It won't spawn! [Re: Prisoner#1]
    #4331440 - 06/24/05 08:20 AM (11 years, 5 months ago)

Quote:

Prisoner#1 said:
one of the jars is showing signs of colonization, be patient




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OfflineDimitrion
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Re: It won't spawn! [Re: LengthyFellow]
    #4331442 - 06/24/05 08:22 AM (11 years, 5 months ago)

Quote:

LengthyFellow said:
Did you wait for the jars to cool COMPLETELY before you innocd? this can take a long time (like 5-8 hours)




I think i did, I waited a whole night and day before inoculation.
That would be long enough. i guess?


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OfflineDimitrion
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Re: It won't spawn! [Re: Prisoner#1]
    #4331446 - 06/24/05 08:25 AM (11 years, 5 months ago)

Quote:

Prisoner#1 said:
one of the jars is showing signs of colonization, be patient




Really?
What are signs of inoculation?
I don't see white fuzz or anything like it, do you?
The picture aint that good :grin:


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OfflineKalix
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Re: It won't spawn! [Re: Dimitrion]
    #4331453 - 06/24/05 08:33 AM (11 years, 5 months ago)

I don't see anything that 100% shows signs of colonization, but you have to wait @ least a few more weeks..


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OfflineDimitrion
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Re: It won't spawn! [Re: Kalix]
    #4331455 - 06/24/05 08:37 AM (11 years, 5 months ago)

Quote:

Kalix said:
I don't see anything that 100% shows signs of colonization, but you have to wait @ least a few more weeks..




Oh oke,
I'll work on my patience :blush:
So there is no need to ad water to the jars?


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Offlinelordoftheshroomz
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Re: It won't spawn! [Re: Dimitrion]
    #4331465 - 06/24/05 08:43 AM (11 years, 5 months ago)

no need to ad water to the jars... but also no need to wait a few weeks... more like a few days before u should see ure first signs of mycelium! but maybe im retarded from all the dxm ive been doing lately! who knows


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Offlinescatmanrav
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Re: It won't spawn! [Re: lordoftheshroomz]
    #4331673 - 06/24/05 11:16 AM (11 years, 5 months ago)



See the spot circled in red....right under the coffee filter theres some colonization it looks like.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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OfflineQuadrupleTree
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Re: It won't spawn! [Re: scatmanrav]
    #4331677 - 06/24/05 11:18 AM (11 years, 5 months ago)

no i think that's foil


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Invisiblebackupwards
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Re: It won't spawn! [Re: QuadrupleTree]
    #4331703 - 06/24/05 11:24 AM (11 years, 5 months ago)

its some kind of plastic wrap or something under that. why is that there? maybe foil but looks like plastic to me.
6 days is not a long time to have waited, give it a little more time before you worry.
peace


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OfflineWillieTomg
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Re: It won't spawn! [Re: backupwards]
    #4331913 - 06/24/05 12:23 PM (11 years, 5 months ago)

My first jars took 4 weeks to colonize.

When we say "patience is a virtue" we aren't kidding. Sometimes it takes only a few days, sometimes it takes several weeks on end. The jars will be done when they're done, and not a second sooner. Have faith my brother...


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Offlinegrow
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Registered: 04/09/05
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Re: It won't spawn! [Re: Dimitrion]
    #4332023 - 06/24/05 12:48 PM (11 years, 5 months ago)

I guess I'm the only one who caught on....

wrap your terrarium with a black trash bag immediately. they are not supposed to be exposed to light @ all

where did you get your spores from?



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Offlinethenewguy05
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Re: It won't spawn! [Re: Dimitrion]
    #4332036 - 06/24/05 12:52 PM (11 years, 5 months ago)

Quote:

Dimitrion said:
Quote:

lepiota said:
Quote:

Dimitrion said:
I used BRF and vermiculite.




no water?




Yes, i did use water. Just enough to make it drip a little bit when squeesed.


you prolly should have stuck to the pf formula. sounds like you put way too much water in there. if you notice in the formula the water content is the same as the rice leaving very little room for ANY water to be sqeezed out of it. pf formula is
1/4 cup brown rice
1/2 cup vermiculite
1/4 cup water


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Offlinescatmanrav
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Re: It won't spawn! [Re: grow]
    #4332323 - 06/24/05 02:08 PM (11 years, 5 months ago)

Quote:

grow said:
I guess I'm the only one who caught on....

wrap your terrarium with a black trash bag immediately. they are not supposed to be exposed to light @ all

where did you get your spores from?






Actually, Mr. Swift, the only reason no one else mentioned it is because its not a big deal :rolleyes:

I still think it looks like mycelium to me :shrug: I do see how it looks like tape that was peeled off or something now though...thats probably what Anno saw too though.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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Offlineprefloppro
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Registered: 05/29/05
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Re: It won't spawn! *DELETED* [Re: scatmanrav]
    #4332366 - 06/24/05 02:19 PM (11 years, 5 months ago)

Post deleted by prefloppro

Reason for deletion: Made a fool out of myself.



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Offlinescatmanrav
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Re: It won't spawn! [Re: prefloppro]
    #4332404 - 06/24/05 02:30 PM (11 years, 5 months ago)

Quote:

prefloppro said:
Not a big deal to keep your jars completly dark? lol. I can personally tell you thatit took me the first time 6 weeks for my jars to colonize because i would look at them every 3 hours or so. My second batch and all new batches take 2 weeks max to 100 colonize, because i leave them alone in complete darkeness, and only look at them once a week. I am 100 percent sure this is why they are colonizing faster. But hey you seem to know it all. Lmao




I dont know it all, but man you want to tell me about colonizing jars?



I mean how are you gonna explain dozens of other people, including myself, who ca get jars to colonize from spores in a week with the light on...or using a light as a source of heat. I'm not gonna argue this with ya buddy, if you want to think thats what it was and not the water content, spores, temperature, air exchange, or any number of the other variables that DO play a role in speed of colonization, then you can keep thinking that. Those who know, know.

By the way, you know the original PF Tek, by Fanaticus, specifically said in the directions to incubate in the light? How do you think all the people who did and do that get along with jars under 2 weeks all the time?


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


Edited by scatmanrav (06/24/05 02:37 PM)


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