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Amazon Shop for: Agar, Malt Extract, Potato Dextrose

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InvisibleTien
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PDA vs. MEA
    #4329509 - 06/23/05 07:38 PM (11 years, 7 months ago)

So I ordered a basic agar culturing kit from Spore Works. It comes with Malt Extract Agar. I know I am going to need more agar soon but I don't know which one is better PDA or MEA. Any suggestions? What are the pros and cons of these agars?

Pluto


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OfflineWorkmanV
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Re: PDA vs. MEA [Re: Tien]
    #4329766 - 06/23/05 08:39 PM (11 years, 7 months ago)

I'd go with the MEA. It seems like freshly made PDA from potatoes you boil yourself is better than the dehydrated product, but its a subtle difference. MEA is reportedly better for woodloving species if that helps.


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Offlineblackout
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Re: PDA vs. MEA [Re: Workman]
    #4331316 - 06/24/05 05:02 AM (11 years, 7 months ago)

Workman- What % ME would you recommend for azures, on agar and in a malt LC?


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Edited by blackout (06/24/05 05:02 AM)


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OfflineWorkmanV
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Re: PDA vs. MEA [Re: blackout]
    #4332098 - 06/24/05 01:10 PM (11 years, 7 months ago)

2% Malt extract for agar and more dilute for broths, something between 0.5% and 0.1% works pretty good.


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Offlineblackout
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Re: PDA vs. MEA [Re: Workman]
    #4332110 - 06/24/05 01:13 PM (11 years, 7 months ago)

Thanks. I was going to do 4%, never heard of 0.1% recommended for a broth before.


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Edited by blackout (06/24/05 01:15 PM)


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InvisibleRoadkill
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Re: PDA vs. MEA [Re: Tien]
    #4332165 - 06/24/05 01:27 PM (11 years, 7 months ago)

I buy all my MEA and Agar Agar from Sporeworks.
I don't buy much from Vendors but these products are top notch!~
I'm a cheap bastard...but I won't use lower grade products for agar work.

I use the MEA...and then make my own PDYA from the Agar Agar.

-PDYA-
500ml water
5 grams of instant potato flakes(Potato Buds Brand)
9 grams agar agar
10 ml honey (Raw natural pure honey - Beemaid Brand)
1 gram of Nutritional Yeast

I switch back and forth with the above PDYA recipy and the MEA.
And have always gotten great results.

tc


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Offlineblackout
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Re: PDA vs. MEA [Re: Roadkill]
    #4332253 - 06/24/05 01:47 PM (11 years, 7 months ago)

Quote:

Roadkill said:
then make my own PDYA from the Agar Agar.




sounds like Johnny 2 times -"I'm gonna go get the papers, get the papers" :smile:

I have used honey too, it is meant to have anti-bacterial properties.

What are the advantages of low sugar % broths?
I have seen studies in threads where the most potent myc was on low % agar. Is there advantages other than saving on ME?


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InvisibleThumpaCap
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Re: PDA vs. MEA [Re: blackout]
    #4333565 - 06/24/05 08:39 PM (11 years, 7 months ago)

i think i've read somewhere that it's good to alternate.. like one time mea then another pda... is that true?? :shrug:


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Amazon Shop for: Agar, Malt Extract, Potato Dextrose

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