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Offlinesdawg02985
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Registered: 06/04/05
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Spores spores spores....
    #4326341 - 06/22/05 09:51 PM (11 years, 5 months ago)

I am about to inoculate a few jars but they were kinda warm from being in my car today and i was wondering if it would be ok to proceed. What temperature is the highest the spore can be placed in b4 they die? thanks


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Offlinegrow
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Registered: 04/09/05
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Re: Spores spores spores.... [Re: sdawg02985]
    #4326348 - 06/22/05 09:53 PM (11 years, 5 months ago)

spores are only killed when sterilized

mycelium, 106F


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OfflineJimmyStewart
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Re: Spores spores spores.... [Re: sdawg02985]
    #4326349 - 06/22/05 09:54 PM (11 years, 5 months ago)

Just wait till the jars are cool. Do it tomorrow.


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Offlinedrugsaregood
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Re: Spores spores spores.... [Re: JimmyStewart]
    #4326359 - 06/22/05 09:57 PM (11 years, 5 months ago)

what were they doing in your car?


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InvisibleThumpaCap
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Re: Spores spores spores.... [Re: drugsaregood]
    #4326385 - 06/22/05 10:02 PM (11 years, 5 months ago)

they were prolly just sitting there.

:stoned:


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Offlinesdawg02985
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Re: Spores spores spores.... [Re: ThumpaCap]
    #4326397 - 06/22/05 10:06 PM (11 years, 5 months ago)

im moving....and i had to transport them. I put them in the freezer for about 25 mins and the jars cooled down...will they be alright?


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Offlinefrank_grimes
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Re: Spores spores spores.... [Re: sdawg02985]
    #4326422 - 06/22/05 10:11 PM (11 years, 5 months ago)

Quote:

sdawg02985 said:
im moving....and i had to transport them. I put them in the freezer for about 25 mins and the jars cooled down...will they be alright?




Yes, they'll be fine.


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OfflineCandyClown
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Re: Spores spores spores.... [Re: frank_grimes]
    #4327359 - 06/23/05 03:16 AM (11 years, 5 months ago)

You may want to re-sterilize just to make sure, but you'll probably be okay. Good luck.


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OfflineWojo
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Re: Spores spores spores.... [Re: CandyClown]
    #4327884 - 06/23/05 11:11 AM (11 years, 5 months ago)

Let em sit for a couple days, see if anything shows its ugly face..


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Offlineblackout
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Re: Spores spores spores.... [Re: grow]
    #4327892 - 06/23/05 11:16 AM (11 years, 5 months ago)

Quote:

grow said:
spores are only killed when sterilized

mycelium, 106F



I think you are thinking of endospores. Cube spores will be killed at fairly low heats.
I think the jars will be fine, certainly no need to resterilize.


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Offlinegrow
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Re: Spores spores spores.... [Re: blackout]
    #4327913 - 06/23/05 11:34 AM (11 years, 5 months ago)

Quote:

blackout said:
Quote:

grow said:
spores are only killed when sterilized

mycelium, 106F



I think you are thinking of endospores. Cube spores will be killed at fairly low heats.
I think the jars will be fine, certainly no need to resterilize.




no, spores.


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Offlineblackout
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Re: Spores spores spores.... [Re: grow]
    #4327967 - 06/23/05 12:00 PM (11 years, 5 months ago)

Quote:

grow said:

no, spores.



Why does every "tek" say to wait for jars to cool. What is your definition of "sterilize". If it means PCing then why bother with syringes. You could simply take a print, stick it in LC and microwave it for 20mins, the spores won't die. Why bother with holes in PF jars? mix spores in with your oringinal verm brf mixture and steam it. They can survive intergalactic travel after all, and the huge temperatures when coming into the atmosphere.
Why are people messing about with multiple agar transfers of wild prints? surely they could pastuerise them or steam them to kill most of the contaminants.

Maybe it is worth trying for anybody with spare spores, next time you knock up an LC just put some in, if it doesnt work just add more.


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Edited by blackout (06/23/05 12:01 PM)


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Offlinegrow
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Re: Spores spores spores.... [Re: blackout]
    #4327988 - 06/23/05 12:09 PM (11 years, 5 months ago)

cool post

only when sterilized - is when most of the spores are killed. if spores are injected into a jar thats been sitting in a hot car, fact of the matter is, you will see growth just as you would injecting into a jar that's room temperature


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Offlinegrow
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Re: Spores spores spores.... [Re: blackout]
    #4327998 - 06/23/05 12:14 PM (11 years, 5 months ago)

Quote:

blackout said:
Quote:

grow said:

no, spores.



Why does every "tek" say to wait for jars to cool. What is your definition of "sterilize". If it means PCing then why bother with syringes. You could simply take a print, stick it in LC and microwave it for 20mins, the spores won't die. Why bother with holes in PF jars? mix spores in with your oringinal verm brf mixture and steam it. They can survive intergalactic travel after all, and the huge temperatures when coming into the atmosphere.
Why are people messing about with multiple agar transfers of wild prints? surely they could pastuerise them or steam them to kill most of the contaminants.

Maybe it is worth trying for anybody with spare spores, next time you knock up an LC just put some in, if it doesnt work just add more.




also I was in no way implicating that it's okie to knock up jars that are still hot from pc, thats just plain dumb


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Offlineblackout
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Re: Spores spores spores.... [Re: grow]
    #4328016 - 06/23/05 12:20 PM (11 years, 5 months ago)

I was being sarcastic in some of my post, but it would be worth trying, like I say, if it doesnt work just add more spores. I remember seeing a post where some guy had a spore collection in his car driving in very hot temps for days, and they survived. Many people said spores wont survive freezing, then Anno tried it and they did survive.
If they could even survive pastuerization it would be useful to know.


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Offlinegrow
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Re: Spores spores spores.... [Re: blackout]
    #4328021 - 06/23/05 12:22 PM (11 years, 5 months ago)

Quote:

blackout said:
I was being sarcastic in some of my post, but it would be worth trying, like I say, if it doesnt work just add more spores. I remember seeing a post where some guy had a spore collection in his car driving in very hot temps for days, and they survived. Many people said spores wont survive freezing, then Anno tried it and they did survive.
If they could even survive pastuerization it would be useful to know.




theres alot of myths out there.. I don't believe one of em... except never mix bleach+ammonia for a disinfectant spray, it killed all the pets in my house


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Offlineprefloppro
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Registered: 05/29/05
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Re: Spores spores spores.... [Re: grow]
    #4328033 - 06/23/05 12:25 PM (11 years, 5 months ago)

Damn thats sad, about your pets. Your mom never told you to never mix cleaning products, or your chemistry teacher? Sorry to hear you lost your pets.


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Offlineblackout
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Re: Spores spores spores.... [Re: prefloppro]
    #4342321 - 06/27/05 11:06 AM (11 years, 5 months ago)

I injected spores into a agar dish about a week ago. I wrapped it in an electric blanket which was turned on the highest setting. I was only supposed to heat it for a minute, but forgot and it was at 45C for about 12 hours. I picked it up today and there is lots of growth on the agar. The agar plate was loose in an autoclave bag so the agar may not have been actually at 45C but it was hot.


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