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Amazon Shop for: ½ Pint Jars, 10cc Syringe, Wild Bird Seed

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Offlineprefloppro
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PE Colonization times
    #4321632 - 06/21/05 07:18 PM (11 years, 8 months ago)

Ok i knocked up some quart wbs jars with PE. Its been two weeks and no growth to this date. I cant find anywhere where it tells you what is the best temp to incubate at and what temp to fruit at, is it just like other strains or do I need to do something special. thanks I currently have them at about 78 - 82 with rh of 75.


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Invisibletahoe
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Re: PE Colonization times [Re: prefloppro]
    #4321638 - 06/21/05 07:19 PM (11 years, 8 months ago)

wtf is pe?


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


Teh=The

I need to proofread


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OfflineRoseM
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Re: PE Colonization times [Re: prefloppro]
    #4321641 - 06/21/05 07:20 PM (11 years, 8 months ago)

That sounds fine for temp.

What substrate did you use, and can your jars breathe?

PE is slow, but it should start colonizing within a week... getting it to pin though... that can take time.


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InvisibleHolydiver
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Re: PE Colonization times [Re: prefloppro]
    #4321645 - 06/21/05 07:20 PM (11 years, 8 months ago)

Two weeks is way too long, something's wrong. Did you use spores or a liquid culture inoculant? And how many CC's per jar? Colonization times depend on isolate vigor, substrate moisture content and temperature, just to name a few of the important ones. Low 80's is perfect for incubation.


--------------------
To find a place to live between the negatives and positives.


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Invisiblebackupwards
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Re: PE Colonization times [Re: tahoe]
    #4321868 - 06/21/05 08:11 PM (11 years, 8 months ago)

phys ed man gives you some penis envy,lol
peace


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Invisibletahoe
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Re: PE Colonization times [Re: backupwards]
    #4321978 - 06/21/05 08:42 PM (11 years, 8 months ago)

and again, wtf is pe?


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


Teh=The

I need to proofread


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InvisibleHolydiver
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Re: PE Colonization times [Re: tahoe]
    #4321981 - 06/21/05 08:43 PM (11 years, 8 months ago)

Penis Envy


--------------------
To find a place to live between the negatives and positives.


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Invisiblefearfect
Registered: 01/15/04
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Re: PE Colonization times [Re: Holydiver]
    #4322074 - 06/21/05 08:59 PM (11 years, 8 months ago)

if I don't see any growth, or very little + stopped in 2 weeks, 99% of the time it won't grow any more, and I end up tossing em :undecided:  I've left them in there for over a month just neglecting em and came back and they just looked shriveled, some had spots of trich.  Its strange to me how I did a 24 jar batch and 12 of them came out fine, while the other 12 stopped


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Offlinedrugsaregood
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Re: PE Colonization times [Re: fearfect]
    #4322101 - 06/21/05 09:04 PM (11 years, 8 months ago)

I had a jar of grain that dried out and stalled early in colonization. I re-sterilized a used syringe with water inside. After it cooled, I added 10cc of water to the jar and got a fully colonized jar with no contaminates. It was a jar of quinoa so it may not be totally applicable, but there is still some hope. I usually don't toss out a jar until I'm sure it's doomed. I've had some interesting comebacks (a green-o-plasty) that did just fine.


--------------------
For "incoherent dribble" (drivel) try www.dictionary.com

Everybody seems to think I'm lazy
I don't mind, I think they're crazy
Running everywhere at such a speed
Till they find there's no need


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Invisiblefearfect
Registered: 01/15/04
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Re: PE Colonization times [Re: drugsaregood]
    #4322220 - 06/21/05 09:24 PM (11 years, 8 months ago)

wow good job :thumbup:


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Offlineprefloppro
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Re: PE Colonization times [Re: fearfect]
    #4322235 - 06/21/05 09:29 PM (11 years, 8 months ago)

Yeah i used a vendor syringe, and i knocked up a couple other quarts with ksss and there almost done colonizing. So i was just wandering if it is just the strain. My jars can breathe, and when I inoculated I shook it up real good, but I was thinking, when i shook it it seemd to leave alot of moisture on the inside of the top of the jar. I am wandering if all of the solution just stuck to the sides of the jar, but even then i think i would see some growth. I dont know I will try to add some water to it, and plan on adding a wedge of myc. to it in the next couple fo days, if I can get my pretri dishs up and running.


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Amazon Shop for: ½ Pint Jars, 10cc Syringe, Wild Bird Seed

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