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Offlinecarl
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Registered: 04/26/05
Posts: 374
Last seen: 16 years, 11 months
grain to grain problems
    #4318405 - 06/20/05 07:40 PM (18 years, 9 months ago)

I have a couple rye bags that were injected with some spores. For some unknown reason only one bag decided to colonize. THat was the last of my spores. Can i spawn the bag that colonized to the a big batch of all the rye i have. Its four bags total but only one colonized.

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InvisibleLouiseLouise
starstruck
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Registered: 11/02/04
Posts: 3,898
Loc: Searching w/my good eye c...
Re: grain to grain problems [Re: carl]
    #4318429 - 06/20/05 07:45 PM (18 years, 9 months ago)

Are they contaminated? I wonder why they didn't germinate?
Yeah, I think you could do that, with the right conditions. Ultra sterility, glove box, ya no.
With those grain bags open, no contaminants can enter, or your done.

GL


--------------------
"That's why you get in close to them, and then take the picture!! Don't be a pussy!" ~CC

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Offlinecarl
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Registered: 04/26/05
Posts: 374
Last seen: 16 years, 11 months
Re: grain to grain problems [Re: LouiseLouise]
    #4319162 - 06/20/05 11:14 PM (18 years, 9 months ago)

What would be the easiest method of doing this?

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Offlinecarl
Revolutionary
Registered: 04/26/05
Posts: 374
Last seen: 16 years, 11 months
Re: grain to grain problems [Re: carl]
    #4319954 - 06/21/05 08:51 AM (18 years, 9 months ago)

Come on... I dont want to waste three bags of rye. Whats my best bet in salvaging them?

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Offlinescatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
Loc: Flag
Last seen: 11 years, 2 months
Trusted Cultivator
Re: grain to grain problems [Re: carl]
    #4319974 - 06/21/05 09:01 AM (18 years, 9 months ago)

Be sterile....if you arent, you'll fail.

Start here:
http://www.shroomery.org/index/par/23426

Search the forum for glove boxes and flow hoods...masically a clean (fresh, new) trash bag, or a rubbermaid container with 2 armholes cut in the side. You need to reduce all airflow where your working...break up the fully colonized bag, and put some colonized grains into each of the fresh bags..you only need 1-10% colonized grains per bag. If your not sterile though, you will lose them all, which is why its important for you to read as much as you can on the subject before attempting it. You probably wont need to use the whole bag too, you can use probably half to case, or spawn to poo, and the other half for g2g transfers.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay

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Offlinecarl
Revolutionary
Registered: 04/26/05
Posts: 374
Last seen: 16 years, 11 months
Re: grain to grain problems [Re: scatmanrav]
    #4322494 - 06/21/05 09:13 PM (18 years, 9 months ago)

Thanks scat you the man.

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