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OfflineHippieChick
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How Many People Love Malt and Dextrose LC's but
    #4311771 - 06/18/05 09:22 PM (15 years, 4 months ago)

hate straining that stuff through those coffee filter's? PC'ing twice. The mess that goes with straining through those filter's, Takes forever to strain. Well, I decided if I was using doubled up coffee filter's, why not just use the coffee maker. Before I did this I asked my Hubby if he might like a new coffee maker for Father's Day, I'm so considerate,lol.

So I put 3 quarts of warm distilled water in a gallon jug, 3 tablespoons each of Malt and Dextrose, shook it up and made some coffee. Ran a pot of just water first, and after.

30 minutes later I had 5 quarts 3/4 full and in the PC. No mess, no fuss, no PC'ing twice. Seems to have worked pretty good.

This is what the filters looked like when finished,




Just thought I'd share as that mess and hassle was starting to get to me,lol.

If you're afraid of messing up your coffee maker, pick up a $3 one at Goodwill and give it a try.

Peace,Love,Happiness and Harmony.
:heart: Hippie Chick  :mushroom2:


--------------------
Peace,Love and Happiness
:heart: HC :mushroom2:

Freedoms just another word for nothing left to lose..............

I LUV My Greenhouse
http://www.shroomery.org/forums/showflat.php/Number/5545848#5545848

My First Pans
http://www.shroomery.org/forums/showflat.php/Number/6212058#6212058


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OfflineWojo
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Re: How Many People Love Malt and Dextrose LC's but [Re: HippieChick]
    #4311857 - 06/18/05 09:47 PM (15 years, 4 months ago)

Have you tried using vacuum filtration? I bet it would make the filtering process alot faster..


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OfflineHippieChick
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Re: How Many People Love Malt and Dextrose LC's but [Re: Wojo]
    #4311879 - 06/18/05 09:53 PM (15 years, 4 months ago)

I don't even use vacuum cleaners,lol.

No haven't tried that, but this pretty much takes care of itself. How long does it take to make a pot of coffee?lol.

Peace,Love,Happiness and Harmony.
:heart: Hippie Chick  :mushroom2:


--------------------
Peace,Love and Happiness
:heart: HC :mushroom2:

Freedoms just another word for nothing left to lose..............

I LUV My Greenhouse
http://www.shroomery.org/forums/showflat.php/Number/5545848#5545848

My First Pans
http://www.shroomery.org/forums/showflat.php/Number/6212058#6212058


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Invisibleagar
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Re: How Many People Love Malt and Dextrose LC's but [Re: HippieChick]
    #4311895 - 06/18/05 09:56 PM (15 years, 4 months ago)

LOL....... :thumbup: :grin:


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Invisiblethemills
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Re: How Many People Love Malt and Dextrose LC's but [Re: agar]
    #4312160 - 06/18/05 11:51 PM (15 years, 4 months ago)

I just dont filter them, and the mycelium usually completely covers the sediment, and thrive from it... :smile:


--------------------
There are 2 possible outcomes: If the result confirms the hypothesis, then you've made a measurement. If the result is contrary to the hypothesis, then you've made a discovery.
-Enrico Fermi
_________________________________________________________________________________________________
Sibling Gatling Gun of Looking at All Sides of the Question


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OfflineLengthyFellow
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Re: How Many People Love Malt and Dextrose LC's but [Re: HippieChick]
    #4312702 - 06/19/05 02:22 AM (15 years, 4 months ago)

I got fed up with malt for this very reason...no matter how much straining hot then cold then pcing twice the stuff always came back once it settled for awhile. Im having a lot of success with karo though  :shrug:


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OfflineKalix
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Re: How Many People Love Malt and Dextrose LC's but [Re: themills]
    #4312709 - 06/19/05 02:24 AM (15 years, 4 months ago)

Quote:

themills said:
I just dont filter them, and the mycelium usually completely covers the sediment, and thrive from it... :smile:




  How do you get sediment chunks into your syringe?


--------------------


My Unitarian Jihad Name is: The Shotgun of Sweet Reason


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OfflineHippieChick
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Re: How Many People Love Malt and Dextrose LC's but [Re: LengthyFellow]
    #4312726 - 06/19/05 02:29 AM (15 years, 4 months ago)

I just took these out a few hours ago. They are the cleanest one's I've had yet, don't know if you can tell from the pic's,



and it was so easy. Thought it was 5, but had 6 jars done and out of the PC in like 45 minutes.

Peace,Love,Happiness and Harmony.
:heart: Hippie Chick  :mushroom2:


--------------------
Peace,Love and Happiness
:heart: HC :mushroom2:

Freedoms just another word for nothing left to lose..............

I LUV My Greenhouse
http://www.shroomery.org/forums/showflat.php/Number/5545848#5545848

My First Pans
http://www.shroomery.org/forums/showflat.php/Number/6212058#6212058


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Invisiblethemills
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Re: How Many People Love Malt and Dextrose LC's but [Re: Kalix]
    #4312823 - 06/19/05 03:17 AM (15 years, 4 months ago)

Quote:

Kalix said:
Quote:

themills said:
I just don't filter them, and the mycelium usually completely covers the sediment, and thrive from it... :smile:




  How do you get sediment chunks into your syringe?




Well, I do LC in Quart jars or bigger... so even if I got a quarter inch of sediment (I have never gotten more than one cm or so) that leaves enough liquid to make a huge amount of syringes. Other than that.. your going use a magnetic stirrer or a piece of broken glass to  separate the mycelium anyways (I hope) which will break up the sediment simultaneously. I mean, how do you suck chunks of mycelium in your syringe?  :stoned:


--------------------
There are 2 possible outcomes: If the result confirms the hypothesis, then you've made a measurement. If the result is contrary to the hypothesis, then you've made a discovery.
-Enrico Fermi
_________________________________________________________________________________________________
Sibling Gatling Gun of Looking at All Sides of the Question


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OfflineKalix
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Re: How Many People Love Malt and Dextrose LC's but [Re: themills]
    #4312825 - 06/19/05 03:18 AM (15 years, 4 months ago)

  I don't.. Was just wondering.. :smile:


--------------------


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Invisibleagar
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Re: How Many People Love Malt and Dextrose LC's but [Re: HippieChick]
    #4313037 - 06/19/05 04:51 AM (15 years, 4 months ago)

If you have the patiance, you don't even need to filter it. Just mix about 20% more than you intend to use, PC & allow it to settle for about 2 days, then carefully pour off the top 80% of the solution into your LC containers & PC again. Ya, I know that's PC'ing it twice. But - for sure - it's sterile.


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OfflineHippieChick
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Re: How Many People Love Malt and Dextrose LC's but [Re: agar]
    #4313070 - 06/19/05 05:11 AM (15 years, 4 months ago)

Up late,lol.

Do you think that 25 minutes at 15psi won't get it sterile? If I'm only going to PC once should I go longer?

Peace,Love,Happiness and Harmony.
:heart: Hippie Chick  :mushroom2:


--------------------
Peace,Love and Happiness
:heart: HC :mushroom2:

Freedoms just another word for nothing left to lose..............

I LUV My Greenhouse
http://www.shroomery.org/forums/showflat.php/Number/5545848#5545848

My First Pans
http://www.shroomery.org/forums/showflat.php/Number/6212058#6212058


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Offlinelepiota
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Re: How Many People Love Malt and Dextrose LC's but [Re: HippieChick]
    #4313083 - 06/19/05 05:33 AM (15 years, 4 months ago)

might not even need to be PCed at all, being a liquid culture the simple boiling in the coffee maker could be sufficient. try not PCing a couple of jars and see if they get contamined!


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Re: How Many People Love Malt and Dextrose LC's but [Re: HippieChick]
    #4313124 - 06/19/05 06:20 AM (15 years, 4 months ago)

20 / 25 minutes in a PC @ 15 psi does the job.


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OfflineIamthewalrus
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Re: How Many People Love Malt and Dextrose LC's but [Re: agar]
    #4313146 - 06/19/05 06:47 AM (15 years, 4 months ago)

I have never used this method but I can vouch for karo being easy as fuck(I tried straw water,millet water, and honey water before karo...none of them worked for me)

heres the karo I use(2tbsp of karo/100ml water) plus a pic of liquid azure AO to show its ability...I have no reason to try anything else at this point







I only STEAM for 5-10 min(and lean towards 5) the less yellow it is the better(less carmalization) at least when it comes to karo


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InvisibleRoadkillM
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Re: How Many People Love Malt and Dextrose LC's but [Re: HippieChick]
    #4313178 - 06/19/05 07:47 AM (15 years, 4 months ago)

Quote:

Wojo said:

Have you tried using vacuum filtration? I bet it would make the filtering process alot faster..

Quote:

HippieChick said:
I don't even use vacuum cleaners,lol.










lmfaoooo sisi


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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OfflineWojo
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Re: How Many People Love Malt and Dextrose LC's but [Re: Roadkill]
    #4313354 - 06/19/05 11:18 AM (15 years, 4 months ago)

^ I got a pretty good laugh out of that too. But as far as vacuum filtration goes, you dont wanna spend a few hundred on a vacuum pump and setup, then buy the glassware necessary to perform a vacuum filtration (filter flask, filter funnel, papers, hose, etc)? Lol, i guess if you have your own lab setup its just kind of convienent.. im gonna do up a few malt/dextrose jars today :smile:


Edited by Wojo (06/19/05 11:19 AM)


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OfflineShdwstr
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Re: How Many People Love Malt and Dextrose LC's but [Re: HippieChick]
    #4313425 - 06/19/05 12:03 PM (15 years, 4 months ago)

Nice filtering method HippieChick, but...

Consider if you will... (In my best Rod Serling voice)

Rather than pre-mixing your malt, dextrose, etc. and then running it through the coffee maker, just put all of the ingredients into the filter basket, just like you would coffee. That way, your only putting distilled water through the coffee maker.
You could start with a Gallon/4litre Jug of distilled water. Fill your coffee maker from this jug. Use 160g (4%) or 120g (3%)of ingredient mix, malt, dextrose, honey, Karo, etc. etc in the filter basket, do a coffee run, then pour the "coffee" back into your Gallon/4Litre Jug of distilled water.
One gallon of LC ready to be put into jars and PC'd :smile:

Shdwstr


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OfflineIamthewalrus
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Re: How Many People Love Malt and Dextrose LC's but [Re: Shdwstr]
    #4313490 - 06/19/05 12:33 PM (15 years, 4 months ago)

seems so complicated...just buy some damn karo and mix with water lol :P works great(least that brand I use does)


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OfflineShdwstr
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Re: How Many People Love Malt and Dextrose LC's but [Re: Iamthewalrus]
    #4313494 - 06/19/05 12:36 PM (15 years, 4 months ago)

Heheheh

Some of us like to play Wally :laugh:


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OfflineIamthewalrus
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Re: How Many People Love Malt and Dextrose LC's but [Re: Shdwstr]
    #4313500 - 06/19/05 12:38 PM (15 years, 4 months ago)

I'm all about getting the best results with the least amount of work...in other words I'm a lazy bastard :P but it takes a lot of work to find those easy methods so I dunno I guess it balances out


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OfflineHippieChick
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Re: How Many People Love Malt and Dextrose LC's but [Re: Iamthewalrus]
    #4313733 - 06/19/05 03:01 PM (15 years, 4 months ago)

I tried Karo, didn't like it as much. Was going to try it again until I tried this. These came out crystal clear. Fast and easy.

Peace,Love,Happiness and Harmony.
:heart: Hippie Chick  :mushroom2:


--------------------
Peace,Love and Happiness
:heart: HC :mushroom2:

Freedoms just another word for nothing left to lose..............

I LUV My Greenhouse
http://www.shroomery.org/forums/showflat.php/Number/5545848#5545848

My First Pans
http://www.shroomery.org/forums/showflat.php/Number/6212058#6212058


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OfflineHippieChick
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Re: How Many People Love Malt and Dextrose LC's but [Re: Shdwstr]
    #4313745 - 06/19/05 03:08 PM (15 years, 4 months ago)

Thanks for the input. Will definitely give that a try. My coffee pot holds a half gallon so I will try it in 1/2 gallon batches. Your advice seems like it will make an easy task even easier, as long as it mixes well. I'll find out. Thanks "Rod",lol.

Peace,Love,Happiness and Harmony.
:heart: Hippie Chick  :mushroom2:


--------------------
Peace,Love and Happiness
:heart: HC :mushroom2:

Freedoms just another word for nothing left to lose..............

I LUV My Greenhouse
http://www.shroomery.org/forums/showflat.php/Number/5545848#5545848

My First Pans
http://www.shroomery.org/forums/showflat.php/Number/6212058#6212058


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OfflineHippieChick
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Re: How Many People Love Malt and Dextrose LC's but [Re: Shdwstr]
    #4313754 - 06/19/05 03:11 PM (15 years, 4 months ago)

Quote:

Shdwstr said:
Heheheh

Some of us like to play Wally :laugh:





Thank God or we would all still be doing cakes,lol.

Peace,Love,Happiness and Harmony.
:heart: Hippie Chick  :mushroom2:


--------------------
Peace,Love and Happiness
:heart: HC :mushroom2:

Freedoms just another word for nothing left to lose..............

I LUV My Greenhouse
http://www.shroomery.org/forums/showflat.php/Number/5545848#5545848

My First Pans
http://www.shroomery.org/forums/showflat.php/Number/6212058#6212058


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InvisibleRoadkillM
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Re: How Many People Love Malt and Dextrose LC's but [Re: Iamthewalrus]
    #4314760 - 06/19/05 08:40 PM (15 years, 4 months ago)

Quote:

Iamthewalrus said:

I'm all about getting the best results with the least amount of work...in other words I'm a lazy bastard :P but it takes a lot of work to find those easy methods so I dunno I guess it balances out




I like using Karo too.

I used to use Honey...but Karo is cheaper.

I'm a cheap and lazy bastard.

:smirk:


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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Offlinedujo
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Re: How Many People Love Malt and Dextrose LC's but [Re: Roadkill]
    #4314830 - 06/19/05 09:01 PM (15 years, 4 months ago)

I don't understand. What exactly are you filtering out?


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Invisibleagar
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Re: How Many People Love Malt and Dextrose LC's but [Re: dujo]
    #4314851 - 06/19/05 09:07 PM (15 years, 4 months ago)

Quote:

dujo said:
I don't understand. What exactly are you filtering out?




heat - sugar = carmalization. Which can turn into itsybitsy chunks of solidified crap = sediment


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OfflineCLUTCH
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Re: How Many People Love Malt and Dextrose LC's but [Re: agar]
    #4314919 - 06/19/05 09:37 PM (15 years, 4 months ago)

I get a lot of sediment even after PCing, cooling, double coffee filtering, and PCing again as you suggest. There was less after running through the coffee filters, but it came right back after the second PC ride.

I still knocked it up. Will this be a problem?


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Re: How Many People Love Malt and Dextrose LC's but [Re: CLUTCH]
    #4314952 - 06/19/05 09:51 PM (15 years, 4 months ago)

No............ :wink:


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OfflineHotnuts
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Re: How Many People Love Malt and Dextrose LC's but [Re: Roadkill]
    #4315208 - 06/19/05 11:13 PM (15 years, 4 months ago)

Quote:

Roadkill said:
Quote:

Iamthewalrus said:

I'm all about getting the best results with the least amount of work...in other words I'm a lazy bastard :P but it takes a lot of work to find those easy methods so I dunno I guess it balances out




I like using Karo too.

I used to use Honey...but Karo is cheaper.

I'm a cheap and lazy bastard.

:smirk:




I'm lazy too. I use vanilla extract now and in MHO it's better than all of'em. :grin: :thumbup:


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Re: How Many People Love Malt and Dextrose LC's but [Re: Hotnuts]
    #4315418 - 06/20/05 12:18 AM (15 years, 4 months ago)

I haven't tried vanilla extract...YET

what are the ratios bro?

I have several bottles of vanilla extract laying around collecting dust.

:smile:


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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OfflineBlue Helix
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Re: How Many People Love Malt and Dextrose LC's but [Re: Roadkill]
    #4315519 - 06/20/05 12:51 AM (15 years, 4 months ago)

Man, it looks like I missed the boat on this LC stuff. I am really late trying it. I just got a mag stirrer from a lab buddy and will be giving it a go with dextrose/malt powder in the next couple days.

Are most of you doing agar isolation, spores, or what? I was going to go with a piece of mycelium off agar without isolation or just flat out spores. Anyone not isolating?


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OfflineIamthewalrus
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Re: How Many People Love Malt and Dextrose LC's but [Re: Roadkill]
    #4315552 - 06/20/05 01:05 AM (15 years, 4 months ago)

hmm I'd also like to try vanilla extract...some honey works some dosen't from what I've heard...the stuff I tried didn't...far as I know all karo works...I think ppl over sterilize a lot of things tho(syringes, lc) karo water only needs like 6mins of steam time and its sterile...using a pc is just beggin for carmalization

btw I have used pieces of shrooms and colonized millet(soaked in h202 for a few seconds before plopplin in) this works 100% of the time for me so far...but I usually use spores


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OfflineIamthewalrus
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Re: How Many People Love Malt and Dextrose LC's but [Re: Iamthewalrus]
    #4315578 - 06/20/05 01:13 AM (15 years, 4 months ago)

btw I like to play too...I've tried a lot of diff shit(not just with lc) I even tried straw and millet water which were the worst of the bunch...but once I find that method that works 99.9% of the time I stick with it and try to perfect it even further if I can(for example double tubs are the best way to fruit cubes that I have seen but I am workin on trying a mono tub right now cause its even easier to build them and find the right tubs)...but if vanilla extract works even better then I would def use it(would be as easy as karo to prepare I imagine)


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Re: How Many People Love Malt and Dextrose LC's but [Re: Roadkill]
    #4315586 - 06/20/05 01:16 AM (15 years, 4 months ago)

1 tablespoon of REAL vanilla extact (not immitation) per 250mills of H20. I used the McCormick brand for these. The LC was ready to use efficiantly in 8 days from a multispore inoculation.


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Re: How Many People Love Malt and Dextrose LC's but [Re: Blue Helix]
    #4315588 - 06/20/05 01:16 AM (15 years, 4 months ago)

I have started LC's from spore print scrapings, multi/spore stringes, inner tissue direct from a stem, isolate off agar, colonized seed/grain from WBS/Rye & rhizo tissue plucked off a casing surface. Everyone worked.


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Re: How Many People Love Malt and Dextrose LC's but [Re: Blue Helix]
    #4315595 - 06/20/05 01:20 AM (15 years, 4 months ago)

I do quite a bit of peti work. Isolating is key to get the most out of a strain IMO. Here's an OI clone on cardboard. I had to use this while I waited for my MEA to come in. LOL! Geeze!


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Re: How Many People Love Malt and Dextrose LC's but [Re: Hotnuts]
    #4315600 - 06/20/05 01:25 AM (15 years, 4 months ago)

That looks totally wicked, hotnutz. I have never seen one quite like it. It's the kind of growth I only dream of.


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Re: How Many People Love Malt and Dextrose LC's but [Re: Hotnuts]
    #4316225 - 06/20/05 08:05 AM (15 years, 4 months ago)

nice!~

I think I'll stick with Karo.

I only have bottles of immitation vanilla extact.

I'm a cheap bastard!~

thank for the info bro.

:smile:

I get great growth from Karo.


good enough for me!~

tc


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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Re: How Many People Love Malt and Dextrose LC's but [Re: Roadkill]
    #4316528 - 06/20/05 11:50 AM (15 years, 4 months ago)

Shit, ya can't bitch about that culture! Looks good! Do you use 1 tablespoon of Karo per 250 also?


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Re: How Many People Love Malt and Dextrose LC's but [Re: Hotnuts]
    #4316721 - 06/20/05 01:05 PM (15 years, 4 months ago)

hrm... how about making a super concentrated sugar syringe.. then just "innoculating" some sterile water LC jars with the apropriate ammount of sterile super concentrated sugar + whatever spore/myc you would normally use???


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Re: How Many People Love Malt and Dextrose LC's but [Re: Hotnuts]
    #4317027 - 06/20/05 02:45 PM (15 years, 4 months ago)

not bitchin bro!~

I'm happy with Karo.

I use :

4cc's karo light
96cc's of warm water.

stirr it up really good.
put a marble into the jar...to break up the mycelium later.
put your lid on.
pressure cook it for 30 mins at 15 psi, or 45 at 10 psi.
after you are done pressure cooking the jar...let it cool down.
after it has cooled down inoculate with 1cc or 2cc's...from your spore syringe.
swirl the jar around a little to mix the spores into the karo water.
then put into your incubator...81 to 84 degrees F.

If I use larger jars I just double the recipe.

and I use a lid with self sealing injection port and a filter patch(round bandaid) for air exchange.

tc


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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