Home | Community | Message Board


Original Seeds Store - Cannabis Seeds
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Contamination Forum

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: ½ Pint Jars, Agar, Petri Dish

Jump to first unread post. Pages: 1
Offlinelepiota
expert infailures
 User Gallery

Registered: 10/27/04
Posts: 266
Loc: Europe
Last seen: 9 years, 8 months
is this petri contamined? (pic)
    #4310099 - 06/18/05 04:39 AM (11 years, 6 months ago)

something alien and strange is growing in my petri dishes.
like a contam, it starts from the sides and moves towards the centre, while the myc starts from the centre and goes to the sides.
unlike contams it's not green or red or purple or any fancy colors: it looks transparent, just a little darker than the agar medium, which is brownish. maybe a bacterial contamuination? do they look like this? it is my first petri contam.
soon this thing and the myc will meet.
what shall i do?



--------------------
SUPPORT YOUR LOCAL FSR!


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinedrugsaregood
PhD B+
 User Gallery

Registered: 04/03/05
Posts: 304
Last seen: 11 years, 6 months
Re: is this petri contamined? (pic) [Re: lepiota]
    #4310261 - 06/18/05 08:33 AM (11 years, 6 months ago)

my first petri too



it looks like contam, but maybe not if it looks transparent. I had some dishes that got patches of that too. maybe fermentation of the agar? looks like you successfully transferred once to this plate already. You may as well cut this one up, innoc and transfer to another petri. The myc growth will likely be effected when it comes to the brown region.


--------------------
For "incoherent dribble" (drivel) try www.dictionary.com

Everybody seems to think I'm lazy
I don't mind, I think they're crazy
Running everywhere at such a speed
Till they find there's no need


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinelepiota
expert infailures
 User Gallery

Registered: 10/27/04
Posts: 266
Loc: Europe
Last seen: 9 years, 8 months
Re: is this petri contamined? (pic) [Re: drugsaregood]
    #4310282 - 06/18/05 08:55 AM (11 years, 6 months ago)

i tossed it, doing 3rd generation today even if petris of the 2nd generation aren't completel? colonized. most of them have the same kind of suspect contam. will make 8 new petris out of the 2 best old ones.
just asking myself how many generations should i do before transferring agar to grain?


--------------------
SUPPORT YOUR LOCAL FSR!


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinedrugsaregood
PhD B+
 User Gallery

Registered: 04/03/05
Posts: 304
Last seen: 11 years, 6 months
Re: is this petri contamined? (pic) [Re: lepiota]
    #4310299 - 06/18/05 09:15 AM (11 years, 6 months ago)

as long as there is clean myc in the middle, you should be able to salvage an infected dish. I cut up one dish with green into 12 jars and only got one jar with green.


--------------------
For "incoherent dribble" (drivel) try www.dictionary.com

Everybody seems to think I'm lazy
I don't mind, I think they're crazy
Running everywhere at such a speed
Till they find there's no need


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinedrugsaregood
PhD B+
 User Gallery

Registered: 04/03/05
Posts: 304
Last seen: 11 years, 6 months
Re: is this petri contamined? (pic) [Re: drugsaregood]
    #4310302 - 06/18/05 09:16 AM (11 years, 6 months ago)

do you use peroxide in your agar? that's the next step for me, but I'm lazy so I've put it off until this weekend, thus the overgrown petri that I showed


--------------------
For "incoherent dribble" (drivel) try www.dictionary.com

Everybody seems to think I'm lazy
I don't mind, I think they're crazy
Running everywhere at such a speed
Till they find there's no need


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinelepiota
expert infailures
 User Gallery

Registered: 10/27/04
Posts: 266
Loc: Europe
Last seen: 9 years, 8 months
Re: is this petri contamined? (pic) [Re: drugsaregood]
    #4310367 - 06/18/05 10:12 AM (11 years, 6 months ago)

i added a little h2o2 to the agar mix this time for the first time (3rd generation). 1.5ml h2o2 to 200ml agar mixture (0.75%), added just before solidification. i will transfer a "healty" part of myc from the previous generation in each dish. my choice for a healthy mycelium follows these rules:

1) myc from a non contamined looking dish over myc from a contamined looking dish
2) rhizomorphic myc over cottony myc
3) faster running myc over slower running myc (running from the centre to the edges of the petri dish of course).

in this way i'm hoping to isolate a fast, rhizomorphic and contam resistant substrain. only after that i will test its fruiting capabilities.

i guess we're going off topic though  :shrug:


--------------------
SUPPORT YOUR LOCAL FSR!


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinedrugsaregood
PhD B+
 User Gallery

Registered: 04/03/05
Posts: 304
Last seen: 11 years, 6 months
Re: is this petri contamined? (pic) [Re: lepiota]
    #4310383 - 06/18/05 10:22 AM (11 years, 6 months ago)

I only have 1 second generation. It was cottony from my second petri from the gills of shroom to a third petri. The third is all cottony but some of the a separate batch look to have some rhizo growing. I started last weekend so I'm working on figuring out what to do this weekend.


--------------------
For "incoherent dribble" (drivel) try www.dictionary.com

Everybody seems to think I'm lazy
I don't mind, I think they're crazy
Running everywhere at such a speed
Till they find there's no need


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinedrugsaregood
PhD B+
 User Gallery

Registered: 04/03/05
Posts: 304
Last seen: 11 years, 6 months
Re: is this petri contamined? (pic) [Re: drugsaregood]
    #4310395 - 06/18/05 10:37 AM (11 years, 6 months ago)

most of that same petri...



--------------------
For "incoherent dribble" (drivel) try www.dictionary.com

Everybody seems to think I'm lazy
I don't mind, I think they're crazy
Running everywhere at such a speed
Till they find there's no need


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop for: ½ Pint Jars, Agar, Petri Dish

Mushrooms, Mycology and Psychedelics >> Contamination Forum

Similar ThreadsPosterViewsRepliesLast post
* Possible contamination...Pics.
( 1 2 all )
mykalt45 2,163 22 05/07/09 11:23 PM
by kaliman
* How to tell if LC is contaminated? *PIC*
( 1 2 all )
droptopsunfire03 4,956 21 12/20/05 09:21 AM
by blackout
* Is This Contaminated (PICS) Dreadlock Rasta 1,419 8 02/21/09 03:00 PM
by archivist
* n00b-Are my BRF cakes contaminated? (pics in post) Abraxis0 5,270 8 07/20/07 12:16 AM
by Abraxis0
* Im starting to get worried about contamination(Pics) PipeMania 2,220 2 01/30/07 06:50 PM
by CureCat
* Agar contamination (pics) Junkaboy 1,436 1 03/04/05 09:43 PM
by Suntzu
* Any Info on this powdery yellow contamination? *PICS* Mana 1,101 1 03/13/08 06:32 PM
by RogerRabbit
* ARE THESE CONTAMINATED (PICS)!? ShroominSpradl 1,782 3 11/17/01 03:22 AM
by flaw

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, cronicr, Tmethyl, Pastywhyte
1,408 topic views. 0 members, 4 guests and 0 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Out-Grow.com - Mushroom Growing Kits & Supplies
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.034 seconds spending 0.003 seconds on 14 queries.